| 质粒名称: | pJKW1148 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 119029 |
| 存储实验室: | Jing-Ke Weng |
|
载体骨架基本信息 |
|
| 载体名称: | pYeDP60 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9282 bp |
| 完整质粒大小: | 10762 bp |
| 载体修饰: | -- |
| 载体类型: | 酵母表达 |
| 筛选标记: | URA3 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PmCYP719A26,PmMTS1 |
| 物种来源: | 卡瓦胡椒 |
| 插入大小: | 1536 bp |
| 基因突变: | -- |
| GenBank编号 | MK058499 |
| 启动子 | GAL10 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson Cloning |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | cttcttttctctaaatattctttc |
| 3' 测序引物 | cttttcggataagaaagcaac |
|
文献引用方法 |
|
| 材料和方法部分: | pJKW1148 was a gift from Jing-Ke Weng (Addgene plasmid # 119029 ; http://n2t.net/addgene:119029 ; RRID:Addgene_119029) |
| 参考文献部分: | The biosynthetic origin of psychoactive kavalactones in kava. Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK. Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 10.1038/s41477-019-0474-0. 10.1038/s41477-019-0474-0 PubMed 31332312 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJKW1148 10763 bp ds-DNA circular SYN 07-DEC-2018
DEFINITION Yeast expression plasmid for PmCYP719A26.
ACCESSION .
VERSION .
KEYWORDS pJKW1148
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10763)
AUTHORS Weng, Jing-Ke
TITLE The biosynthetic origin of psychoactive kavalactones in kava
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10763)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10763
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 102..371
/gene="S. cerevisae PGK1"
/label=PGK1 terminator
/note="transcription terminator for 3-phosphoglycerate
kinase gene"
primer_bind complement(395..411)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(395..411)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(408..430)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 419..435
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(443..473)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 488..509
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter complement(2259..2634)
/gene="S. cerevisiae ADE2"
/label=ADE2 promoter
primer_bind complement(2827..2844)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2998..3586)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3078..3097)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3757..4617)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4380..4399
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4618..4722)
/gene="bla"
/label=AmpR promoter
primer_bind 4790..4808
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4846..4868)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4968..4987
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5181..5203
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5195..5212
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 5196..5212
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 5777..7119
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind complement(6740..6787)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 7500..7715
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 7716..8519
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
protein_bind 8776..8893
/label=UAS
/bound_moiety="Gal4"
/note="upstream activating sequence mediating
Gal4-dependent induction"
ORIGIN
1 cgcgggggat ctcccatgtc tctactggtg gtggtgcttc tttggaatta ttggaaggta
61 aggaattgcc aggtgttgct ttcttatccg aaaagaaata aattgaattg aattgaaatc
121 gtagatcaat ttttttcttt tctctttccc catcctttac gctaaaataa tagtttattt
181 tattttttga atatttttta tttatatacg tatatataga ctattattta tcttttaatg
241 attattaaga tttttattaa aaaaaaattc gctcctcttt taatgccttt atgcagtttt
301 tttttcccat tcgatatttc tatgttcggg ttcagcgtat tttaagttta ataactcgaa
361 aattctgcgt tcgttaaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
541 cgggaaacct gtcgtgccag agcttcgtaa ccgacagttt ctaacttttg tgctttgaca
601 agaacttctt cttcttgctt taataaaaac tgttccattt tcgttgtata acttgaatca
661 taagcgccaa gcagtctgac agccaacagc gcagcgttcg tactattatt aatagcgacg
721 gtagctactg gaacacctct aggcatttgc acaattgaat gtaaagaatc tactccatct
781 agacaagaac cttttacggg cacaccgatg acaggaagtg gtgtcattgc agccaccata
841 cctggcaagt gagcagcccc accagctcca gcgataattg ttttaattcc acgcttgctt
901 gcggaaatag catatgctga catcctatgt ggagttctat gagcagagac tattgtcact
961 tcaaatggaa cgccaaaatc ttttaaaacc gcacatgcgg cagacattac cggcaagtca
1021 gagtctgatc ccatgatgat tccaaccaat ggtttgacca ttgcttccaa gtccaacttt
1081 tgagcgacag agattttgat tggaatatca gttctacctg taatgtagtt cagcctttgt
1141 tcacattccg ccatactgga ggcaataata tttatgtgac ctacttttct gttaggtcta
1201 gactcttttc catataagta cactgaggaa cctggagtcg ccaatgctct ttcgcaagtt
1261 tctagctctt tatcttttgt atgtttgtct ccaagaacat ttagcataat ggcgttcgtt
1321 gtaatggtgg agaaagatgt gaaattcttt ggcattggca aatccaatat tgatctcaaa
1381 tgagcttcaa attgagaagt gacgcaagca tcaatggtat aatgtccaga gttgtgaggc
1441 cttggggcaa tttcgttaat aagcaattcc cctgtttcta aatagaacat ttccacacca
1501 aatataccac aaccgggaaa agatttgatt gcattttctg ccaacaactt cgccttaagt
1561 tgaacggagt ccggaactct agcaggcgca taacataagt cacaaatatt gtccttgtgg
1621 atagtctcta caattgggta agaaaacact aaaccgttaa cagatctcac aatcatgact
1681 gctaattctt tagtaaatgg tgcccatttt tcggcgtaca aaggacgatc cttcagtact
1741 tccaaagctt ccggaatcat ttccttattc tttacaacga agttacctct tccatcgtat
1801 gccaaagtcc tcgacttcaa gacgaatgga aaacccaaat ctcttccaac attcaatagg
1861 gacgtctcac tggcttgttc cacaggaaca ctttgggtaa ctgctatacc atttttgatt
1921 aaatgctctt tttgaatata tttgtcttgt atcaatctga ttgtttctgg agaagggtaa
1981 atttttaatt tgggatgttt tacttgaaga ttctttagtg taggaacatc aacatgctca
2041 atctcaatcg ttagcacatc acatttttca gctagttttt cgatatcaag aggattggaa
2101 aaggagccat taacgtggtc attggagttg cttatttgtt tggcaggaga attttcagca
2161 tctagtatta ccgtcttaat gttgagcctg tttgctgcct caacaatcat acgtcccaat
2221 tgtccccctc ctaatatacc aactgttcta gaatccatac ttgattgttt tgtccgattt
2281 tcttgttttt cttgattgtt atagtaggat gtacttagaa gagagatcca acgattttac
2341 gcaccaattt atacatgaaa tgctccataa tattgtccat ttagttctta ataaaaggtc
2401 agcaagagtc aatcacttag tattacccgg ttcgtagcca tgcaacaaga gtcatttgtc
2461 agcatagctg taataatcaa tcatgacgta agaaatgtat cataattaaa agttgttaaa
2521 gatgtcagtg ttatgttggt gttacaaaat tctcggcttc tcactaatat ttaatatctc
2581 ttaaatttta tctgtctttg attcttttaa gaaaagttat gtattattca agaaaaagtc
2641 aattccgcat caaaaggtaa aatttatata aactgcttta aaatttcatg aaactaggca
2701 acttttcgaa atgatctttt tcgagcatga agtttctttt ataataacct ggtcaaaagc
2761 tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
2821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
2881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
2941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
3001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
3061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
3121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
3181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
3241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
3301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
3361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
3421 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
3481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
3541 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
3601 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
3661 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
3721 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
3781 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
3841 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
3901 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
3961 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
4021 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
4081 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
4141 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
4201 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
4261 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
4321 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
4381 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
4441 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
4501 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
4561 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
4621 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
4681 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
4741 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
4801 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
4861 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
4921 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
4981 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
5041 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
5101 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
5161 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat
5221 tccacggact atagactata ctagtatact ccgtctactg tacgatacac ttccgctcag
5281 gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca
5341 aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta gaccgagaaa
5401 gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac
5461 gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca
5521 aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa
5581 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
5641 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
5701 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
5761 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
5821 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
5881 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
5941 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
6001 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
6061 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
6121 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
6181 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
6241 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
6301 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
6361 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
6421 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
6481 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
6541 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
6601 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
6661 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
6721 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
6781 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
6841 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
6901 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
6961 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
7021 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
7081 ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt accgagaaac
7141 tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg tcctggccac
7201 ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg ttaggccctt
7261 cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc cattttttat
7321 agcaaagatt gaataaggcg catttttctt caaagcttta ttgtacgatc tgactaagtt
7381 atcttttaat aattggtatt cctgtttatt gcttgaagaa ttgccggtcc tatttactcg
7441 ttttaggact ggttcagaat taattctcat gtttgacagc ttatcatcgc gataagcttt
7501 tcaattcatc attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg
7561 attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat
7621 atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc
7681 acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac
7741 gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc
7801 aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg
7861 aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt
7921 ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct
7981 tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg
8041 tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta
8101 ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga
8161 tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg
8221 ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg
8281 gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca
8341 agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg
8401 acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg
8461 aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa
8521 aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat
8581 tatatcagtt attacccgat caaaaatcat cgcttcgctg attaattacc ccagaaataa
8641 ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt tcatttgaag
8701 gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt
8761 agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac
8821 cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca cccgctcggc
8881 ggcttctaat ccgtacttca atatagcaat gagcagttaa gcgtattact gaaagttcca
8941 aagagaaggt ttttttaggc taatcgacct cgagcagatc cgccaggcgt gtatatagcg
9001 tggatggcca ggcaacttta gtgctgacac atacaggcat atatatatgt gtgcgacgac
9061 acatgatcat atggcatgca tgtgctctgt atgtatataa aactcttgtt ttcttctttt
9121 ctctaaatat tctttcctta tacattaggt cctttgtagc ataaattact atacttctat
9181 agacacgcaa acacaaatac acacactaaa ttaccggatc catggagcaa gctcaatggg
9241 tcgacccaac tctgctccct gcatttgtgg gcatcatctt cttcttcctt ggcatgttct
9301 ttggaaggag ttctttggga gctgggaagg gtgcagcgcc tagaagcacc agttctaccg
9361 agtggccaga cggccctcca aagctgccca tcatcggcaa cctgcaccag ctcaacaaag
9421 gcggggagct ggtccaccac aacctcgcca agctcgccca gtcctacgac cgcgccatga
9481 ccatctgggt cggcagctgg ggccccatga tcgtcgtcag cgacgccgac cttgcatggg
9541 aggtcctcgt caccaagtcg ccggacttcg ccggccgggt gctctccaag ctctcgcact
9601 tgttcaacgc caactacaac accgtcgtcg cctacgacgc cgggccgcaa tggcagtcgc
9661 tccggcgagg tctgcagcac gggccgctcg gccccgccca tgtttctgcg caggctcgtt
9721 tccacgaaga agacatgaag ctcctggtga gcgacatgat gagagcagca cagaaaggtg
9781 gtagcaatgg agtggttgaa cctctggcct atgtccggcg agccactatc cgatttctgt
9841 ctcgtctatg ctttggggag gccttcaacg acgaggcgtt cgtggagggg atggacgaag
9901 cagtggagga gaccatcgga gccactggcc atgcacgcat cctcgacgcc ttctacttca
9961 ctcgccacct ccctatcatc cgccgcagct tcatagatac cgtcaacgcc aagaagaaga
10021 tcgagagcct tgtccggccg ttgctctccc ggccggcgcc accggggtct tacctccact
10081 tcctcctttc caccgacgcg ccggagaata tgatcatctt tcgaatattc gaagtctact
10141 tgctgggcgt ggacagcacc gcctccacca ccacatgggc tctcgccttc ctcgtctcca
10201 accaacaggc gcaggagaag ctccacaatg agctcgccca gtactgcgcc agccagaaca
10261 atcagatcat caaagcagac gacgtcggaa agctgtcgta cctgctcggg gtagtgaagg
10321 agacgatgag gatgaagccg atagcgccgc tggccgtccc ccacaagacg ctcaaggaga
10381 cgatgctcga cggaaagagg gtggcggcgg gaacgacggt ggtagtgaac ctctatgccg
10441 tccactacaa cccgaagcta tggccggagc cggagcagtt ccgcccggag aggttcgtgg
10501 tcggcgccag cggcggcaat ggtggggggt cttccgagta catgctgcag tcgtacctgc
10561 cctttggagg ggggatgagg tcctgcgcag ggatggaggt gggaaagttg caggtggcga
10621 tggtcgtggc caaccttgtg atggcattta aatggttgcc ggaggaggag gggaagatgc
10681 cggacctggc tgaagacatg accttcgtgc tcatgatgaa gaagccattg gctgccaaaa
10741 tcgttccacg tgcatgagaa ttc
//
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