| 质粒名称: | pMSP3535 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46886 |
| 存储实验室: | Gary Dunny |
|
载体骨架基本信息 |
|
| 载体名称: | pMSP3535 |
| 载体抗性: | 红霉素 |
| 空载体大小: | 8353 bp |
| 载体修饰: | See associated article for detailed construction information |
| 载体类型: | 细菌表达；Nisin诱导表达；革兰氏阳性菌穿梭载体 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 启动子 |
PnisA
|
| 克隆方法: | 限制性内切酶 |
| 3' 测序引物 | T7; pBR322ori-F (5'-GGGAAACGCCTGGTATCTTT-3') |
|
文献引用方法 |
|
| 材料和方法部分: | pMSP3535 was a gift from Gary Dunny (Addgene plasmid # 46886 ; http://n2t.net/addgene:46886 ; RRID:Addgene_46886) |
| 参考文献部分: | Improved vectors for nisin-controlled expression in gram-positive bacteria. Bryan EM, Bae T, Kleerebezem M, Dunny GM. Plasmid. 2000 Sep;44(2):183-90. 10.1006/plas.2000.1484 PubMed 10964628 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMSP3535 8353 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled
Inducible Expression.
ACCESSION .
VERSION .
KEYWORDS pMSP3535
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8353)
AUTHORS Bryan EM, Bae T, Kleerebezem M, Dunny GM
TITLE Improved vectors for nisin-controlled expression in gram-positive
bacteria.
JOURNAL Plasmid. 2000 Sep;44(2):183-90.
PUBMED 10964628
REFERENCE 2 (bases 1 to 8353)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8353
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 7090..7678
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7579..7598
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7832..7849
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 7936..7955
/label=T7
/note="T7 promoter, forward primer"
promoter 7936..7954
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 agatctgatc cgtagcggtt ttcaaaattt gcaaccagga atgaattact atccctttta
61 tcaagaagcg caaaagaaaa acgaaatgat acaccaatca gtgcaaaaaa agatataatg
121 ggagataaga cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat cgaaacagca
181 aagaatggcg gaaacgtaaa agaagttatg gaaataagac ttagaagcaa acttaagagt
241 gtgttgatag tgcagtatct taaaattttg tataatagga attgaagtta aattagatgc
301 taaaaatttg taattaagaa ggagtgatta catgaacaaa aatataaaat attctcaaaa
361 ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac
421 cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat
481 aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa
541 attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc
601 taacaaacag aggtataaaa ttgttgggag tattccttac catttaagca cacaaattat
661 taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat ctgattgttg aagaaggatt
721 ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc
781 gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag
841 tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata
901 tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca
961 gtttcatcaa gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca
1021 agtattgtct atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc
1081 gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta
1141 tactactgac agcttccaag gagctaaaga ggtccctagc gcttagaatc gctttaggaa
1201 acacgatcca gtccaataat cgtcgataaa aacttttgaa aaaggttggt gaaattacct
1261 acttttggaa taatcacaaa tcacaagtga ttaatcacaa atcacaagtg attaatcact
1321 tgtttattaa gatattaaaa gctataattt aaataaagcg tgaattttat tacacaaaaa
1381 gaggggggag aaacttggaa ctagcattta gagaaagctt aaaaaagatg agaggtacca
1441 aatcaaaaga aaaattctcc caagaattag aaatgagtag atcaaattat tcacgaatag
1501 aatcaggaaa atcagatcca accataaaaa cactagaaca aattgcaaag ttaactaact
1561 caacgctagt agtggattta atcccaaatg agccaacaga accagaacca gaaacagaat
1621 cagaacaagt aacattggat ttagaaatgg aagaagaaaa aagcaatgac ttcgtgtgaa
1681 taatgcacga aatcgttgct tatttttttt taaaagcggt atactagata taacgaaaca
1741 acgaactgaa tagaaacgaa aaaagagcca tgacacattt ataaaatgtt tgacgacatt
1801 ttataaatgc atagcccgat aagattgcca aaccaacgct tatcagttag tcagatgaac
1861 tcttccctcg taagaagtta tttaattaac tttgtttgaa gacggtatat aaccgtacta
1921 tcattatata gggaaatcag agagttttca agtatctaag ctactgaatt taagaattgt
1981 taagcaatca atcggaaatc gtttgattgc tttttttgta ttcatttata gaaggtggag
2041 tttgtatgaa tcatgatgaa tgtaaaactt atataaaaaa tagtttattg gagataagaa
2101 aattagcaaa tatctataca ctagaaacgt ttaagaaaga gttagaaaag agaaatatct
2161 acttagaaac aaaatcagat aagtattttt cttcggaggg ggaagattat atatataagt
2221 taatagaaaa taacaaaata atttattcga ttagtggaaa aaaattgact tataaaggaa
2281 aaaaatcttt ttcaaaacat gcaatattga aacagttgaa tgaaaaagca aaccaagtta
2341 attaaacaac ctattttata ggatttatag gaaaggagaa cagctgaatg aatatccctt
2401 ttgttgtaga aactgtgctt catgacggct tgttaaagta caaatttaaa aatagtaaaa
2461 ttcgctcaat cactaccaag ccaggtaaaa gcaaaggggc tatttttgcg tatcgctcaa
2521 aatcaagcat gattggcggt cgtggtgttg ttctgacttc cgaggaagcg attcaagaaa
2581 atcaagatac atttacacat tggacaccca acgtttatcg ttatggaacg tatgcagacg
2641 aaaaccgttc atacacgaaa ggacattctg aaaacaattt aagacaaatc aataccttct
2701 ttattgattt tgatattcac acggcaaaag aaactatttc agcaagcgat attttaacaa
2761 ccgctattga tttaggtttt atgcctacta tgattatcaa atctgataaa ggttatcaag
2821 catattttgt tttagaaacg ccagtctatg tgacttcaaa atcagaattt aaatctgtca
2881 aagcagccaa aataatttcg caaaatatcc gagaatattt tggaaagtct ttgccagttg
2941 atctaacgtg taatcatttt ggtattgctc gcataccaag aacggacaat gtagaatttt
3001 ttgatcctaa ttaccgttat tctttcaaag aatggcaaga ttggtctttc aaacaaacag
3061 ataataaggg ctttactcgt tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac
3121 aagtagatga accctggttt aatctcttat tgcacgaaac gaaattttca ggagaaaagg
3181 gtttaatagg gcgtaataac gtcatgttta ccctctcttt agcctacttt agttcaggct
3241 attcaatcga aacgtgcgaa tataatatgt ttgagtttaa taatcgatta gatcaaccct
3301 tagaagaaaa agaagtaatc aaaattgtta gaagtgccta ttcagaaaac tatcaagggg
3361 ctaataggga atacattacc attctttgca aagcttgggt atcaagtgat ttaaccagta
3421 aagatttatt tgtccgtcaa gggtggttta aattcaagaa aaaaagaagc gaacgtcaac
3481 gtgttcattt gtcagaatgg aaagaagatt taatggctta tattagcgaa aaaagcgatg
3541 tatacaagcc ttatttagtg acgaccaaaa aagagattag agaagtgcta ggcattcctg
3601 aacggacatt agataaattg ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga
3661 ttaaaccagg aagaaatggt ggcattcaac ttgctagtgt taaatcattg ttgctatcga
3721 tcattaaagt aaaaaaagaa gaaaaagaaa gctatataaa ggcgctgaca aattcttttg
3781 acttagagca tacattcatt caagagactt taaacaagct agcagaacgc cctaaaacgg
3841 acacacaact cgatttgttt agctatgata caggctgaaa ataaaacccg cactatgcca
3901 ttacatttat atctatgata cgtgtttgtt ttttctttgc tgtttagcga atgattagca
3961 gaaatataca gagtaagatt ttaattaatt attaggggga gaaggagaga gtagcccgaa
4021 aacttttagt tggcttggac tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc
4081 agtgagagcg aagcgaacac ttgatttttt aattttctat cttttatagg tcattagagt
4141 atacttattt gtcctataaa ctatttagca gcataataga tttattgaat aggtcattta
4201 agttgagcat attagaggag gaaaatcttg gagaaatatt tgaagaaccc gattacatgg
4261 attggattag ttcttgtggt tacgtggttt ttaactaaaa gtagtgaatt tttgattttt
4321 ggtgtgtgtg tcttgttgtt agtatttgct agtcaaagtg attaaataga attctcatgt
4381 ttgacagctt atcatcggag ctccgatgat aagctgtcca aacatgagaa ttccccggct
4441 ttaggtatag tgtgtatctc aatccttggt atattgaaaa gaaagactaa aaattgatag
4501 attatatttc ttcagaatga atggtataat gaagtaatga gtactaaaca atcggaggta
4561 aagtggtgta taaaatttta atagttgatg atgatcagga aattttaaaa ttaatgaaga
4621 cagcattaga aatgagaaac tatgaagttg cgacgcatca aaacatttca cttcccttgg
4681 atattactga ttttcaggga tttgatttga ttttgttaga tatcatgatg tcaaatattg
4741 aagggacaga aatttgtaaa aggattcgca gagaaatatc aactccaatt atctttgtta
4801 gtgcgaaaga tacagaagag gatattataa acggcttagg tattggtggg gatgactata
4861 ttactaagcc ttttagcctt aaacagttgg ttgcaaaagt ggaagcaaat ataaagcgag
4921 aggaacgcaa taaacatgca gttcatgttt tttcagagat tcgtagagat ttaggaccaa
4981 ttacatttta tttagaagaa aggcgagtct gtgtcaatgg tcaaacaatt ccactgactt
5041 gtcgtgaata cgatattctt gaattactat cacaacgaac ttctaaagtt tatacgagag
5101 aggatattta tgatgacgta tatgatgaat attctaatgc actttttcgg tcaatctcgg
5161 agtatattta tcagattagg agtaagtttg caccatacga tattaatccg ataaaaacgg
5221 ttcggggact tgggtatcag tggcatgggt aaaaaatatt caatgcgtcg acggatatgg
5281 caagctgtca ttgaaattat cataggtact tgtctactta tcctgttgtt actgggcttg
5341 actttctttc tacgacaaat tggacaaatc agtggttcag aaactattcg tttatcttta
5401 gattcagata atttaactat ttctgatatc gaacgtgata tgaaacacta cccatatgat
5461 tatattattt ttgacaatga tacaagtaaa attttgggag gacattatgt caagtcggat
5521 gtacctagtt ttgtagcttc aaaacagtct tcacataata ttacagaagg agaaattact
5581 tatacttatt caagcaataa gcatttttca gttgttttaa gacaaaacag tatgcctgaa
5641 tttacaaatc atacgcttcg ttcaatttct tataatcaat ttacttacct tttctttttt
5701 cttggtgaaa taatactcat tattttttct gtctatcatc tcattagaga attttctaag
5761 aattttcaag ccgttcaaaa gattgcattg aagatggggg aaataactac ttttcctgaa
5821 caagaggaat caaaaattat tgaatttgat caggttctga ataacttata ttcgaaaagt
5881 aaggagttag ctttccttat tgaagcggag cgtcatgaaa aacatgattt atccttccag
5941 gttgctgcac tttcacatga tgttaagaca cctttaacag tattaaaagg aaatattgaa
6001 ctgctagaga tgactgaagt aaatgaacaa caagctgatt ttattgagtc aatgaaaaat
6061 agtttgactg tttttgacaa gtattttaac acaatgatta gttatacaaa acttttgaat
6121 gatgaaaatg attacaaagc gacaatctcc ctggaggatt ttttgataga tttatcagtt
6181 gagttggaag agttgtcaac aacttatcaa gtggattatc agctagttaa aaaaacagat
6241 ttaaccactt tttacggaaa tacattagct ttaagtcgag cacttatcaa tatctttgtt
6301 aatgcctgtc agtatgctaa agagggtgaa aaaatagtca gtttgagtat ttatgatgat
6361 gaaaaatatc tctattttga aatctggaat aatggtcatc ctttttctga acaagcaaaa
6421 aaaaatgctg gaaaactatt tttcacagaa gatactggac gtagtgggaa acactatggg
6481 attggactat cttttgctca aggtgtagct ttaaaacatc aaggaaactt aattctcagt
6541 aatcctcaaa aaggtggggc agaagttatc ctaaaaataa aaaagtaatt tagtaatctc
6601 taaggattac tttttttgtt tcggcaaact attaactggc gaactactta ctctagcttc
6661 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
7021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
7081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
7141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
7201 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
7261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
7321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
7381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
7441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
7501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
7741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
7801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
7861 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggttaac
7921 ctggcttatc gaaattaata cgactcacta tagggagacc ggcctcgagt ctagaagcgc
7981 tgtcgacgag ctcgcatgcg aattcctgca gcccgggact agtggtaccg gatccatgca
8041 gagtctcctg ttttacaacc gggtgtacat agcgaaatac ttgtaatgcg tggtgatgca
8101 cctgaatctt tcttcgaaac agataccaaa tccaagctaa aatcttttgt actcattttg
8161 agtgcctcct tataatttat tttgtagttc cttcgaacga aatcattgta tctaacaaac
8221 ttcagaattt aatcagagcc gtttattatg ctcgcgttat cgacaataat attattacca
8281 atactttctc aagatagaat taagactgtt ttagatttgt taatgtttct attgtcagta
8341 tagttataag act
//
