| 出品公司: | -- |
|---|---|
| 载体名称: | pXMJ19 |
| 质粒类型: | 谷氨酸棒杆菌/大肠杆菌穿梭质粒 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6601bp |
| 5' 测序引物及序列: |
LacI-R: GGCATACTCTGCGACATCGT; Tac promoter: GAGCGGATAACAATTTCACACAGG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 筛选标记: | -- |
| 备注: | -- |
| Genbank: | AJ133195.1 |
| 稳定性: | 稳定 |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pXMJ19载体质粒基本信息
pXMJ19质粒图谱载体图谱和pXMJ19载体序列质粒序列多克隆位点信息
pXMJ19质粒载体简介
pXMJ19质粒序列载体序列
LOCUS pXMJ19 6601 bp DNA circular SYN 10-DEC-1999
DEFINITION Shuttle vector pXMJ19 (Corynebacterium glutamicum / E. coli).
ACCESSION AJ133195
VERSION AJ133195.1 GI:6562908
KEYWORDS cat gene; chloramphenicol acetyltransferase; lacIQ gene.
SOURCE Shuttle vector pXMJ19
ORGANISM Shuttle vector pXMJ19
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1
AUTHORS Jakoby,M.J., Ngouto-Nkili,C.E. and Burkovski,A.
TITLE Construction and application of new Corynebacterium glutamicum
vectors
JOURNAL BioTechniques 13, 437-441 (1999)
REFERENCE 2 (bases 1 to 6601)
AUTHORS Jakoby,M.J.
TITLE Direct Submission
JOURNAL Submitted (22-FEB-1999) Jakoby M.J., Institut fuer Biochemie,
Universitaet zu Koeln, Zuelpicher Str. 47, 50674 Koeln, GERMANY
FEATURES Location/Qualifiers
source 1..6601
/organism="Shuttle vector pXMJ19"
/mol_type="other DNA"
/db_xref="taxon:89017"
/lab_host="Corynebacterium glutamicum / E. coli"
gene 67..492
/gene="rrnB"
terminator 67..492
/gene="rrnB"
/experiment="experimental evidence, no additional details
recorded"
gene 967..1539
/gene="ori pUC"
rep_origin 967..1539
/gene="ori pUC"
gene 1724..4278
/gene="ori pBL1"
rep_origin 1724..4278
/gene="ori pBL1"
gene complement(4410..5069)
/gene="cat"
CDS complement(4410..5069)
/gene="cat"
/experiment="experimental evidence, no additional details
recorded"
/codon_start=1
/transl_table=11
/product="chloramphenicol acetyl transferase"
/protein_id="CAB62546.1"
/db_xref="GI:6562909"
gene complement(5135..5229)
/gene="psiGA1"
promoter complement(5135..5229)
/gene="psiGA1"
/experiment="experimental evidence, no additional details
recorded"
gene complement(5282..>6253)
/gene="lacIq"
CDS complement(5282..>6253)
/gene="lacIq"
/codon_start=1
/transl_table=11
/product="LacIq protein"
/protein_id="CAB62547.1"
/db_xref="GI:6562910"
ORIGIN
1 aattaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
61 cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
121 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
181 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
241 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
301 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
361 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
421 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
481 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
781 tgatgagcac ttttgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
1021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
1081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
1141 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
1201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
1261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
1321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
1381 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
1441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
1501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
1561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
1621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg gggtgggcga
1681 agaactccag catgagatcc ccgcgctgga ggatcatcca gccattcggg gtcgttcact
1741 ggttcccctt tctgatttct ggcatagaag aacccccgtg aactgtgtgg ttccgggggt
1801 tgctgatttt tgcgagactt ctcgcgcaat tccctagctt aggtgaaaac accatgaaac
1861 actagggaaa cacccatgaa acacccatta gggcagtagg gcggcttctt cgtctagggc
1921 ttgcatttgg gcggtgatct ggtctttagc gtgtgaaagt gtgtcgtagg tggcgtgctc
1981 aatgcactcg aacgtcacgt catttaccgg gtcacggtgg gcaaagagaa ctagtgggtt
2041 agacattgtt ttcctcgttg tcggtggtgg tgagcttttc tagccgctcg gtaaacgcgg
2101 cgatcatgaa ctcttggagg ttttcaccgt tctgcatgcc tgcgcgcttc atgtcctcac
2161 gtagtgccaa aggaacgcgt gcggtgacca cgacgggctt agcctttgcc tgcgcttcta
2221 gtgcttcgat ggtggcttgt gcctgcgctt gctgcgcctg tagtgcctgt tgagcttctt
2281 gtagttgctg ttctagctgt gccttggttg ccatgcttta agactctagt agctttcctg
2341 cgatatgtca tgcgcatgcg tagcaaacat tgtcctgcaa ctcattcatt atgtgcagtg
2401 ctcctgttac tagtcgtaca tactcatatt tacctagtct gcatgcagtg catgcacatg
2461 cagtcatgtc gtgctaatgt gtaaaacatg tacatgcaga ttgctggggg tgcagggggc
2521 ggagccaccc tgtccatgcg gggtgtgggg cttgccccgc cggtacagac agtgagcacc
2581 ggggcaccta gtcgcggata ccccccctag gtatcggaca cgtaaccctc ccatgtcgat
2641 gcaaatcttt aacattgagt acgggtaagc tggcacgcat agccaagcta ggcggccacc
2701 aaacaccact aaaaattaat agtccctaga caagacaaac ccccgtgcga gctaccaact
2761 catatgcacg ggggccacat aacccgaagg ggtttcaatt gacaaccata gcactagcta
2821 agacaacggg cacaacaccc gcacaaactc gcactgcgca accccgcaca acatcgggtc
2881 taggtaacac tgagtaacac tgaaatagaa gtgaacacct ctaaggaacc gcaggtcaat
2941 gagggttcta aggtcactcg cgctagggcg tggcgtaggc aaaacgtcat gtacaagatc
3001 accaatagta aggctctggc ggggtgccat aggtggcgca gggacgaagc tgttgcggtg
3061 tcctggtcgt ctaacggtgc ttcgcagttt gagggtctgc aaaactctca ctctcgctgg
3121 gggtcacctc tggctgaatt ggaagtcatg ggcgaacgcc gcattgagct ggctattgct
3181 actaagaatc acttggcggc gggtggcgcg ctcatgatgt ttgtgggcac tgttcgacac
3241 aaccgctcac agtcatttgc gcaggttgaa gcgggtatta agactgcgta ctcttcgatg
3301 gtgaaaacat ctcagtggaa gaaagaacgt gcacggtacg gggtggagca cacctatagt
3361 gactatgagg tcacagactc ttgggcgaac ggttggcact tgcaccgcaa catgctgttg
3421 ttcttggatc gtccactgtc tgacgatgaa ctcaaggcgt ttgaggattc catgttttcc
3481 cgctggtctg ctggtgtggt taaggccggt atggacgcgc cactgcgtga gcacggggtc
3541 aaacttgatc aggtgtctac ctggggtgga gacgctgcga aaatggcaac ctacctcgct
3601 aagggcatgt ctcaggaact gactggctcc gctactaaaa ccgcgtctaa ggggtcgtac
3661 acgccgtttc agatgttgga tatgttggcc gatcaaagcg acgccggcga ggatatggac
3721 gctgttttgg tggctcggtg gcgtgagtat gaggttggtt ctaaaaacct gcgttcgtcc
3781 tggtcacgtg gggctaagcg tgctttgggc attgattaca tagacgctga tgtacgtcgt
3841 gaaatggaag aagaactgta caagctcgcc ggtctggaag caccggaacg ggtcgaatca
3901 acccgcgttg ctgttgcttt ggtgaagccc gatgattgga aactgattca gtctgatttc
3961 gcggttaggc agtacgttct cgattgcgtg gataaggcta aggacgtggc cgctgcgcaa
4021 cgtgtcgcta atgaggtgct ggcaagtctg ggtgtggatt ccaccccgtg catgatcgtt
4081 atggatgatg tggacttgga cgcggttctg cctactcatg gggacgctac taagcgtgat
4141 ctgaatgcgg cggtgttcgc gggtaatgag cagactattc ttcgcaccca ctaaaagcgg
4201 cataaacccc gttcgatatt ttgtgcgatg aatttatggt caatgtcgcg ggggcaaact
4261 atgatgggtc ttgttgttgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
4321 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
4381 gaagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
4441 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
4501 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
4561 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
4621 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
4681 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
4741 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
4801 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaactccgga
4861 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
4921 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
4981 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
5041 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgtc
5101 gaagctcggc ggatttgtcc tactcaagct gatccgacaa aatccacaca ttatcccagg
5161 tgtccggatc ggtcaaatac gctgccagct catagaccgt atccaaagca tccggggctg
5221 atccccggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
5281 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgtggtttgc cccagcaggc
5341 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
5401 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
5461 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
5521 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
5581 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
5641 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
5701 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
5761 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
5821 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
5881 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
5941 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
6001 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
6061 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
6121 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
6181 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
6241 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcaccattcg atggtgtcaa
6301 cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg atccgggctt atcgactgca
6361 cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg
6421 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
6481 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
6541 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagaat
6601 t
//
