| 质粒名称: | pEGFP-N1-TrkB |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 32500 |
| 存储实验室: | Rosalind Segal |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TRKB |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | 无（销毁时未知） |
| 3' 克隆位点: | 无（销毁时未知） |
| 5' 测序引物 | CMV-Fwd |
| 3' 测序引物 | EGFP-N |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-N1-TrkB was a gift from Rosalind Segal (Addgene plasmid # 32500 ; http://n2t.net/addgene:32500 ; RRID:Addgene_32500) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEGFP-N1-TrkB 7270 bp ds-DNA circular SYN 07-JUN-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEGFP-N1-TrkB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7270) TITLE Rosalind Segal Lab Plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 7270) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 7, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7270 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 3210..3219 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3216..3935 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3261..3282) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3522..3541) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3869..3890 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 4058..4179 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4095..4114) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4149..4168 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4186..4641) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4323..4344) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4535..4554 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4668..4772 /gene="bla" /label=AmpR promoter promoter 4774..5131 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4797..4817) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4982..5117 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5044..5063 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5166..5960 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5220..5239) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5830..5849 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6148..6167) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6192..6239 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6568..7156 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7057..7076 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggccccc 661 tcccccctcc ggcgggggac gcctggctca gcgtagggac acgcactctg actgactggc 721 actggcagct cgggatgtcg ccctggccga ggtggcatgg acccgccatg gcgcggctct 781 ggggcttatg cttgctggtc ttgggcttct ggagggcttc tcttgcctgc cccatgtcct 841 gcaaatgcag caccactagg atttggtgta ccgagccttc tcctggcatc gtggcatttc 901 cgaggttgga acctaacagc attgacccag agaacatcac cgaaattctc attgcaaacc 961 agaaaaggtt agaaatcatc aatgaagatg atgtcgaagc ttacgtgggg ctgaaaaacc 1021 ttacaattgt ggattccggc ttaaagtttg tggcttacaa ggcgtttctg aagaacggca 1081 acctgcggca catcaatttc actcgaaaca agctgacgag tttgtccagg agacatttcc 1141 gccaccttga cttgtctgac ctgatcctga cgggtaatcc gttcacgtgt tcctgtgaca 1201 tcatgtggct caagactctc caggagacga aatccagccc cgacactcag gatttgtatt 1261 gcctcaatga gagcagcaag aatacccctc tggcgaacct gcagattccc aattgtggtc 1321 tgccgtctgc acgtctggcc gctcctaacc tcacggtgga ggaagggaag tctgtgacca 1381 tttcctgcag cgtcgggggt gacccgctcc ccaccttgta ctgggacgtt gggaatttgg 1441 tttccaaaca catgaatgaa acaagccaca cacagggctc cttaaggata acaaacattt 1501 catcggatga cagtgggaaa caaatctctt gtgtggcaga aaacctcgtc ggagaagatc 1561 aagactctgt gaacctcact gtgcattttg caccaaccat cacatttctc gaatctccaa 1621 cctcagacca ccactggtgc atcccattca ctgtgagagg caaccccaag ccagcacttc 1681 agtggttcta caacggagcc atactgaatg aatccaagta catctgtacc aaaatacacg 1741 tcaccaatca cacggagtac cacggctgcc tccagctgga taaccccact catatgaata 1801 atggagacta caccctaatg gccaagaatg aatatgggaa ggacgagaga cagatttctg 1861 ctcacttcat gggccggcct ggagttgact atgagacaaa cccaaattac cctgaagtcc 1921 tctatgaaga ctggaccacg ccaactgaca tcggggatac tacaaacaaa agtaatgaga 1981 tcccctccac ggatgttgct gaccaaacca atcgggagca tctctcggtc tatgctgtgg 2041 tggtgattgc ctctgtggta ggattctgcc tgctggtgat gctgcttctg ctcaagttgg 2101 cgagacattc caagtttggc atgaaaggcc cagcttccgt catcagcaac gacgatgact 2161 ctgccagccc tctccaccac atctccaacg ggagcaacac tccgtcttct tcggagggcg 2221 ggcccgatgc tgtcatcatt gggatgacca agatccctgt cattgaaaac ccccagtact 2281 tcggtatcac caacagccag ctcaagccgg acacatttgt tcagcacatc aagagacaca 2341 acatcgttct gaagagggag cttggagaag gagcctttgg gaaagttttc ctagcggagt 2401 gctataacct ctgccccgag caggataaga tcctggtggc cgtgaagacg ctgaaggacg 2461 ccagcgacaa tgctcgcaag gactttcatc gcgaagccga gctgctgacc aacctccagc 2521 acgagcacat tgtcaagttc tacggtgtct gtgtggaggg cgacccactc atcatggtct 2581 ttgagtacat gaagcacggg gacctcaaca agttccttag ggcacacggg ccagatgcag 2641 tgctgatggc agagggtaac ccgcccaccg agctgacgca gtcgcagatg ctgcacatcg 2701 ctcagcaaat cgcagcaggc atggtctacc tggcatccca acacttcgtg caccgagacc 2761 tggccacccg gaactgcttg gtaggagaga acctgctggt gaaaattggg gacttcggga 2821 tgtcccggga tgtatacagc accgactact accgggttgg tggccacaca atgttgccca 2881 tccgatggat gcctccagag agcatcatgt acaggaaatt caccaccgag agtgacgtct 2941 ggagcctggg agttgtgttg tgggagatct tcacctacgg caagcagccc tggtatcagc 3001 tatcaaacaa cgaggtgata gaatgcatca cccagggcag agtccttcag cggcctcgca 3061 cgtgtcccca ggaggtgtac gagctgatgc tgggatgctg gcagcgggaa ccacacacaa 3121 ggaagaacat caagaacatc cacacactcc ttcagaactt ggcgaaggcg tcgcccgtct 3181 acctggacat cctaggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt 3241 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 3301 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 3361 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 3421 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 3481 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 3541 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 3601 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3661 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 3721 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 3781 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 3841 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 3901 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat 3961 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 4021 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 4081 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4141 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta 4201 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 4261 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 4321 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 4381 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg 4441 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 4501 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 4561 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 4621 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4741 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 4801 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 4861 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 4921 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 4981 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 5041 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 5101 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 5161 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 5221 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 5281 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 5341 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 5401 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 5461 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 5521 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 5581 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 5641 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 5701 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 5761 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 5821 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 5881 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 5941 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 6001 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 6061 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 6121 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 6181 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 6241 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 6301 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 6361 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 6421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 6481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 6541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 6601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 6661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 6721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 6781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 6841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 6901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 7021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 7081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 7141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 7201 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 7261 cgccatgcat //
