| 质粒名称: | pJYS2.1-crtYf |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89077 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 and pTRCmob |
| 载体出品公司: | Sheng Yang |
| 完整质粒大小: | 4280 bp |
| 载体类型: | 细菌表达、 CRISPR |
| 载体抗性: | 大观霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | crRNA of crtYF |
|
文献引用方法 |
|
| 材料和方法部分: | pJYS2.1-crtYf was a gift from Sheng Yang (Addgene plasmid # 89077 ; http://n2t.net/addgene:89077 ; RRID:Addgene_89077) |
| 参考文献部分: | CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. 10.1038/ncomms15179 PubMed 28469274 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJYS2.1-crtYf 4280 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Constitutive transcription of crRNA of crtYF. CRISPR of C.
glutamicum..
ACCESSION .
VERSION .
KEYWORDS pJYS2.1-crtYf
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4280)
AUTHORS Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X,
Li Y, Wang R, Yang S
TITLE CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
JOURNAL Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
PUBMED 28469274
REFERENCE 2 (bases 1 to 4280)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4280
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 3147..3232
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3324..3351
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(3445..3462)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3616..4204)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3696..3715)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 actaataacg taacgtgact ggcaagagat atttttaaaa caatgaatag gtttacactt
61 actttagttt tatggaaatg aaagatcata tcatatataa tctagaataa aattaactaa
121 aataattatt atctagataa aaaatttaga agccaatgaa atctataaat aaactaaatt
181 aagtttattt aattaacaac tatggatata aaataggtac taatcaaaat agtgaggagg
241 atatatttga atacatacga acaaattaat aaagtgaaaa aaatacttcg gaaacattta
301 aaaaataacc ttattggtac ttacatgttt ggatcaggag ttgagagtgg actaaaacca
361 aatagtgatc ttgacttttt agtcgtcgta tctgaaccat tgacagatca aagtaaagaa
421 atacttatac aaaaaattag acctatttca aaaaaaatag gagataaaag caacttacga
481 tatattgaat taacaattat tattcagcaa gaaatggtac cgtggaatca tcctcccaaa
541 caagaattta tttatggaga atggttacaa gagctttatg aacaaggata cattcctcag
601 aaggaattaa attcagattt aaccataatg ctttaccaag caaaacgaaa aaataaaaga
661 atatacggaa attatgactt agaggaatta ctacctgata ttccattttc tgatgtgaga
721 agagccatta tggattcgtc agaggaatta atagataatt atcaggatga tgaaaccaac
781 tctatattaa ctttatgccg tatgatttta actatggaca cgggtaaaat cataccaaaa
841 gatattgcgg gaaatgcagt ggctgaatct tctccattag aacataggga gagaattttg
901 ttagcagttc gtagttatct tggagagaat attgaatgga ctaatgaaaa tgtaaattta
961 actataaact atttaaataa cagattaaaa aaattataaa aaaattgaaa aaatggtgga
1021 aacacttttt tcaatttttt tgttttatta tttaatattt gggaaatatt cattctaatt
1081 ggtaatcaga ttttagaaaa caataaaccc ttgcatatga tatcgatgta cagatcccat
1141 caatcctgcc tatttgccac gtttaacaag gtagttaagc gttcatttac gaagaaaaca
1201 cgataagctg cacaaatacc tgaaaaagtt gaacgccccg tgagcgggaa ctcacagggc
1261 gtcggctaac ccccagtcat cagctgggag aaagcactca agacatgact ctagccgatc
1321 cgcaggacac agtcacagct agcgcgtgga aattttccgc cgatctgttc gacacccacc
1381 ccgaactagc gctgcgctca cgcggctgga cggcagaaga tcgccgcgaa ctgctcgctc
1441 acctgggacg cgaaagcttc cagggcagca agacaagaga tttcgcgagc gcctggatta
1501 aaaacccgga taccggcgaa acccaaccaa agctctaccg ggctggctca aaagcgctga
1561 cgcggtgcca gtacgttgcg ctgacgcacg cgcaacatgc cgcggtgatc gtgcttgaca
1621 tcgatgtgcc cagccaccag gccggcggga agattgagca cgtaaacccg caggtctacg
1681 cgattttaga gaaatgggca cgcctagaaa aagcgccggc ttggatcggc gtgaatccgc
1741 tgagcgggaa atgccagctc atctggctca ttgacccggt gtatgccgca gcaggtaaaa
1801 ccagcccaaa tatgcgcctg ctggctgcaa cgacggaaga aatgactcgt gttttcggcg
1861 ctgaccaggc tttttcgcat aggctgagcc ggtggccgct gcacgtctca gacgatccga
1921 cagcctataa atggcactgc cagcatgatc gtgtggatcg gctggccgac ctaatggaga
1981 ttgctcgaac gatgaccgga tcacagaagc cgaaaaagta cattgagcag gacttttcca
2041 gcggacgcgc ccgcattgaa gcggcacaac gcgccaccgc agaagccaag gcgctagcga
2101 ttttggacgc gagcctgccg agcgccctgg acgcgtccgg cgacctgatc gacggcgtgc
2161 gagtgctctg gacaaatcca gagcgcgcag cgcgcgacga gaccgcgttt cgccacgcgt
2221 tgaccgtggg ataccagctc aaagctgctg gtgagcgcct aaaagatgcc aagatcatcg
2281 acgcgtatga agtggcgtac aacgttgccc aggcggtcgg tgcagacggc cgggagccgg
2341 atcttcccgc catgcgtgat cgcctgacga tggcgcgtcg tgtgcgcggc tacgtggcta
2401 aaggccagcc agtcgtccct gctcgtcggg tggaaacgca gagcagccga gggcggaaag
2461 ctctagcgac gatggggcga cggggcgcag ctacatcgaa tgcacgcaga tgggctgacc
2521 cagaaagtaa gtatgcgcag gagacgcgac agcgattagc ggaagcaaac aaacgccgag
2581 aaatgacagg cgagttgctc gaacttcgcg tcaaaactgc gatcctggat gcccgttctc
2641 aatcggttgc tgatccctcg actcgtgagc ttgcaggcga actaggtgtc agtgaaaggc
2701 gcatccaaca agtcagaaag gcacttggaa tggaagctaa acgcggccgt ccacgggctg
2761 aaaactaata aacgaaacac cgtcagcaga aaacggttcc cccctttagg ggtcccgtcc
2821 ttgctctggc tctcacttgc cctcaccctc cgctatccac gggctgaaaa ctaataaacg
2881 aaacaccgtc agcagaaaac ggttcccccc ctttagggtg tctcgctcct agctctgatc
2941 cctccccggt tcctccccgg cctgattttt aagggggctc acgctgtcgg cagagaacgg
3001 ttcccccgcc ttctgctctg gctcttcctc gactccctcc ccctcaaaaa tctcctcgag
3061 tctagattga cagctagctc agtcctaggt ataatggatc cgaatttcta ctgttgtaga
3121 tcaggcaacc atagggcagg aaatttaaat aaaacgaaag gctcagtcga aagactgggc
3181 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
3241 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
3301 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
3361 acaaactctt aagcttcata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
3421 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4021 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
4081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4141 cgctggtagc ggtggttttt ttgtttgtaa gcagcagatt acgcgcagaa aaaaaggatc
4201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4261 ttaagggatt ttggtcatga
//
