| 质粒名称: | pXY1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90487 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pIMP1-Pthl |
| 完整质粒大小: | 5435bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pXY1 was a gift from Sheng Yang (Addgene plasmid # 90487 ; http://n2t.net/addgene:90487 ; RRID:Addgene_90487) |
| 参考文献部分: | I-SceI-mediated scarless gene modification via allelic exchange in Clostridium. Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S. J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. 10.1016/j.mimet.2014.11.004 PubMed 25451462 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pXY1 5435 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid with thl(CA_C2873) promoter for gene expression in
Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii
NCIMB8052.
ACCESSION .
VERSION .
KEYWORDS pXY1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5435)
AUTHORS Zhang N, Shao L, Jiang Y, Gu Y, Li Q, Liu J, Jiang W, Yang S
TITLE I-SceI-mediated scarless gene modification via allelic exchange in
Clostridium.
JOURNAL J Microbiol Methods. 2015 Jan;108:49-60. doi:
10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15.
PUBMED 25451462
REFERENCE 2 (bases 1 to 5435)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5435
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1173..1189)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1173..1189)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1186..1208)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1197..1213
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1221..1251)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1266..1287
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1404..1421)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1575..2163)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1655..1674)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2334..3194)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2957..2976
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3195..3299)
/gene="bla"
/label=AmpR promoter
primer_bind 3367..3385
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(3423..3445)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 3545..3564
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3758..3780
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3772..3789
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3773..3789
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 aaattatcga ttcacaaaaa ataggtacac gaaaaacaag ttaagggatg cagtttatgc
61 atcccttaac ttacttatta aataatttat agctattgaa aagagataag aattgttcaa
121 agctaatatt gtttaaatcg tcaattcctg catgttttaa ggaattgtta aattgatttt
181 ttgtaaatat tttcttgtat tctttgttaa cccatttcat aacgaaataa ttatacttct
241 gtttatcttt gtgtgatatt cttgattttt ttctatttaa tctgataagt gagctattca
301 ctttaggttt aggatgaaaa tattctcttg gaaccatact taatatagaa atatcaactt
361 ctgccattaa aaataatgcc aatgagcgtt ttgtatttaa taatctttta gcaaacccgt
421 attccacgat taaataaatc tcatcagcta tactatcaaa aacaattttg cgtattatat
481 ccgtacttat gttataaggt atattaccaa atattttata ggattggttt ttaggaaatt
541 taaactgcaa tatatccttg tttaaaactt ggaaattatc gtgatcaaca agtttatttt
601 ctgtagtttt gcataattta tggtctattt caatggcagt tacgaaatta cacctctgta
661 ctaattcaag ggtaaaatgc ccttttcctg agccgatttc aaagatatta tcatgttcat
721 ttaatcttat atttgtcatt attttatcta tattatgttt tgaagtaata aagttttgac
781 tgtgttttat atttttctcg ttcattataa ccctctttat tttttcctcc ttataaaatt
841 agtataatta tagcacgagc tctgataaat atgaacatga tgagtgatcg ttaaatttat
901 actgcaatct gatgcgatta ttgaataaaa gatatgagag atttatctag tttctttttt
961 tacaagaaaa aagaaagttc ttaaaggttt tatacttttg gtcgtagagc acacggttta
1021 acgacttaat tacgaagtaa ataagtctag tgtgttagac tttaatgttt ttttaaggca
1081 ttagtgcatt taagcgtcag agcatggctt tatgccgaga aaactattgg ttggaatggc
1141 gtgtgtgtta gccaaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
1261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
1321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
1381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
1441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
1501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
1561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
1621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
1681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
1741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
1801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
1861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
1921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
1981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
2041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
2101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
2161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
2221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
2281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
2341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
2401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
2461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
2521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
2581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
2641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
2701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
2761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
2821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
2881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
2941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
3001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
3061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
3121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
3181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
3241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
3301 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
3361 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
3421 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
3481 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
3541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
3601 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
3661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
3721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
3781 acggccagtg aattcccggg gatcctctaa ctaacctcct aaattttgat acggggtaac
3841 agataaacca tttcaatcta tttcataagt tccatagttt atccctaatt tatacgtttt
3901 ctctaacaac ttaattatac ccactattat tatttttatc aatatatttt gttaaaaact
3961 gcagcacatt aagtatatac tattattaaa ctattctata tacttaattt attttaatag
4021 aaaaacataa tatcataata acttcaaaat taaactttat ttatgatttc atacttgact
4081 ttgattttag aaaggatata ctttttagca gatttggaaa cggctttgga cgtagtttgc
4141 ccatagatga acaaacaaac tacatccaaa aattatactt ttcccttcat tggtatccgt
4201 atttttacat cttaatagcg tatgtattac aacacaccta aacaacgacc ttacggtctg
4261 ctactgcata tcctagcttg attgtttagt tgcctcaact atgcttaacc ctaccccgaa
4321 ctcttttttt attgtgggtt ttcgtcgtga agtcccaccg acacataatc ataacataag
4381 atgtattatg aaaatgcgag tgactatcct tttgtatcgg ctcactacac cacagatata
4441 ttttttagtg catactgtgt cggcactctc aatattaaat taataaataa attatttttt
4501 ctttttactc ttctttacat gagctttttt aaagctcctt gcataatatt taatgcatgt
4561 acgttctttt ttctgttctt cctctgtaaa acatctcatt tttatggcac accatccata
4621 tcggttcatc tttgaacaat taatacattg gactttccct ttatgtaaac atcttgactc
4681 attgtattta ctgcaatagg ttgctactgt tttatcatga atcatcaacg aatgtatttt
4741 gcatatttca gtatctttaa taaactcttt gcaattttta caagttttca tatacgccct
4801 tcttttcata aattaattta tgaattctat gtattccaag gagcttttta aagctccccc
4861 tttcgtacta cttagctaca agcactataa aagtcataat gtttactgct aaagtcaata
4921 atgatattgc taataccttg ttatttgata agatactgct ttcctctgtc actttgctca
4981 ccccctttca ttttcataaa ttaattatga aaaaataaat atacttctaa tttttatcaa
5041 ataaaaaagc ctttgcgtac tgcttccaat acacaaaggc tataaacttc taaatcttac
5101 ttattgcaat ttacatttat atctgttaag ataatctcat aaattgaata tatatagtta
5161 atgtttctct tgtatgtcgg tacatttgaa atattgctat agatagagtt ctctaacggc
5221 ttgatgtgtt ggtagcacat ttaagttttg gcttatatac taagttggta gcttaatata
5281 taagagctga ggacttattt ttttattaaa tttttcaact tgtctatatt ttaacccgta
5341 attgaataca taacaagtat ttttttttgt attcaattaa acattcataa atgagtataa
5401 ttaatcatac taaattctat aattttcttt tctgt
//
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