| 质粒名称: | crestin |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 89394 |
| 存储实验室: | Marnie Halpern |
|
载体骨架基本信息 |
|
| 载体名称: | bluescript |
| 完整质粒大小: | 6904 bp |
| 载体类型: | in situ probe细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | crestin |
| 物种来源: | D. rerio (zebrafish) |
| 插入大小: | 3900 bp |
|
文献引用方法 |
|
| 材料和方法部分: | crestin was a gift from Marnie Halpern (Addgene plasmid # 89394 ; http://n2t.net/addgene:89394 ; RRID:Addgene_89394) |
| 参考文献部分: | Genes dependent on zebrafish cyclops function identified by AFLP differential gene expression screen. Rubinstein AL, Lee D, Luo R, Henion PD, Halpern ME. Genesis. 2000 Jan;26(1):86-97. 10.1002/(SICI)1526-968X(200001)26:1<86::AID-GENE11>3.0.CO;2-Q PubMed 10660676 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS crestin 6904 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION For in situ hybridization, linearize with Sac I use T7 polymerase.
ACCESSION .
VERSION .
KEYWORDS crestin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6904)
AUTHORS Rubinstein AL, Lee D, Luo R, Henion PD, Halpern ME
TITLE Genes dependent on zebrafish cyclops function identified by AFLP
differential gene expression screen.
JOURNAL Genesis. 2000 Jan;26(1):86-97.
PUBMED 10660676
REFERENCE 2 (bases 1 to 6904)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6904
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(3953..3969)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3953..3969)
/label=pBluescriptSK
/note="For pBluescript vector"
promoter complement(4006..4024)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4045..4061)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4045..4061)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4058..4080)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4069..4085
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4093..4123)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4138..4159
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4276..4293)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4447..5035)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4527..4546)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5206..6066)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5829..5848
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6067..6171)
/gene="bla"
/label=AmpR promoter
rep_origin 6198..6653
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6285..6304)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6495..6516
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6780..6802
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6794..6811
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6795..6811
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6818..6837
/label=T7
/note="T7 promoter, forward primer"
promoter 6818..6836
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gatttattgg gctacactac gataaatgat actaactacg taataagcaa acaacaaaca
61 aacacacaca ttcacacata cacacaaagg cagttcagag gatgcaaaat gagttgacaa
121 cgtcccaaat taattctaat acttcttgca agatcttaga ataacacctg agaaaccaca
181 aaagcagaga tatggcttat ctttaactgc ttaagatcaa tctgcacaag atcagcaaga
241 gacaacatta gtttgtgtta cttgcgccct ttttgctgaa gtcgaatctc cctcgccgac
301 tggccttggg gaaacacggt gctcctgatt gggtggtttc tcgcttcaat gacgtctttg
361 ggattcacgc gctcgtgaat ggctgttgtc ctgggttacc gaggagacgg acagctgaga
421 gtcggtttgc ggaagttcat gcagcttcgg gagtcccgaa acttaagagg tttgcgtgga
481 agttcgtagt gacccaatcg cgtggcggcg cggtgtccgg gcaggcgaaa gggggcagag
541 agcaaaagca tgcgcaagct agctgatgca aaaagcaaag tttctttgtc gcagggtgtt
601 tttaagtgac ctggtctggt tcgtccccat ggcactgtct gccaatgagc agttgccatg
661 aagggcgggc cacaccccgt actgacgtag ggaaggtcaa taattaacag actgcatgta
721 gatttcaagt ggtaaaactc ttgcaagatt gaaactatct tacattaaac gttataagat
781 taatactgtt tgtactacta gtttttcatt tacaccaacg tattgcaaat gtcactagct
841 ttcgttcaat accagacatc atacatttca gacacataca gaacatttta ctctgttata
901 agggttaaaa caacacattg attaacctgg ttgatttgca ataaacagca tatgaggaac
961 attatggcat attattcaaa gatacatctg ccttagttct aacattttaa gagattgtct
1021 ttcattcata tgcattaatg tttctagaga tctctttgtc tctacaaaat tgtgcttcta
1081 gcaggatgtg cccaaggcga gcacatgacc aggagtcaat caaaagtctc gtgaaatggt
1141 ttccgctgaa gatcctgtaa gggccattgt gttttaattt aactgattta attaatcatt
1201 ggctagttag ccgaagggta ccagaacatc tgcggagaag tactggtcag aggagcccaa
1261 aatcaattgg cgctctgtta tcctgatggt ataagccctc aagctagttc aatgaatgag
1321 accattggag atgcccaaga tttcagattc aacttgtgtt gaccactgaa aaaggccgat
1381 gagttcctac agtccccctt tgactggtga tgttcccggt gtgaacattg gcaggcactg
1441 gaacaaacag gcagaagcag agagaacagg caaacacaag acacaaacaa catcatcact
1501 aactgaatct tggtgcaggg tttaggaact ttggagtgct cgggttagca atgctcaagg
1561 tgtagaagag catagtttag tatgatcaga atttaggctt ttaagactag ccgaggtatt
1621 gaaggttcaa cgaagttgta taggccttat ctcaatcagt aaagaattta atttttggtc
1681 agcgttatcg catctaagca tgcgcgagat gtggggcgtg cacatttgaa caacttttgg
1741 atggccttta ctcgctcgtg gaggaagagg gcaagtgtca aagtcaacgt ataccctaca
1801 atgctatttt gacgagagtc aaaccaataa atttcattct ttaccctcaa tccctttttc
1861 gttctggatt gaagatgaag aaatgtggta aacaaatctt ttgagttttc gcctaaaacg
1921 tgtcgtcaga caggtgccac aacgttaaat taccaataca gcgatcacac ctttaaggca
1981 ttgatccgtg aggcttggtg aaaggagtca atcctaattc tgagctctgg acttaaacat
2041 catctgtggg ggttccctga tgaggcaaga gaaattaaaa gacagagaga aagagaaaga
2101 aaagaacact gagacataag gacattgagt gaggttaaga caaacaaatg tcacatgtaa
2161 tagctcctct ctactagaga ttggtcctag tagtgcagtg gaggggagag tgcatgtatt
2221 gggtttagga aacatagcta gggctaggtg tcttcctaaa atggcacaaa tgacaaaaac
2281 aggttagcgt taaaataaga gtttaggata gtgggtctgt gtcagcaggt tggtccctcc
2341 ccctttctgg tcacttggag ttgagggact ggagttggca tggcacaggt aggatttgga
2401 ttgattctga gttcagctgc gtggcaaacg atcaagacag cactcttagg aattggaagg
2461 gtttcatcca caagtgtttg tgtgatgcag tggtgacctg aggcatcagg tgttgagtta
2521 gcgtctaact ctggtgttca ggtacagtct attgagtctt tgaagttatg gtgcttacag
2581 ttctctgtaa gtgaggtgaa gaggtgctgt ttagtgaggt cgttgcttgt caggcagaat
2641 caggttttcc tttcctttcc cacttccgat ccgcttccct tcgttggaag aactctttca
2701 ggatcatttt catcagttct tgagggtcaa aacatggtga tgttgtccct tgtggggatt
2761 cagttggagc tcgatcagcg tggaatcttt gtgagttctt tcgttgacct cttgggctgg
2821 ttgtatcaag gtgatgttgc tttaccttgg aagtaccact tgattcccac gagcttgtcc
2881 ccctttggtt tacaaatgag gtagacctgt tccacggtct ctcccggtga cgttcaggtg
2941 agtgttgtcg tccagacagt cctttccagc gacggttcca ctgtttgggt ctggtatcag
3001 catgacagtc tcgttgtctg ttgtaagacg acacattcca ctcactgtga ggtagtctag
3061 cattgtctgg gccttgggca ccctcaaggg ccagatcttg actttgagtg ttgaagtcat
3121 aaacagcagg gattttggca ctcttctctg aagccgtctt ttgtttgacg tatgctttat
3181 gtgccaaatc tcgtatttgc tgagtgttca tggtgtgggg gcatgcaaga accccaagat
3241 ggtggcttac cccaggatgg aggtttctca ggaagaggat tttgaagttc aggtcctctt
3301 ccatctcagg ttcattgcgg gtgccgaagt aggctcgtct gagtctgctg tagtaggctt
3361 gagaagactc atggcgactt tgtttcgtct ccagggcagc tacaagtcct tgttcagact
3421 cgatgtcatc aaactctctg atgagggctt ctcggagcag gaggtagtca gtctttgtgt
3481 gagccggctg acggtccagg aagctgcgca cttcagggct ggaggttgct cggagcaaat
3541 acagtctatc tttgtcagtg acattgggtc gcatttccag gtgaaagtca atgtcctgca
3601 gataggcgtg aacatcttgg ctacctggca aattcggcgt gaacttgccc aggttcttga
3661 ctagcttgtc aaggtctttg aggtccaggc cacagggtga tacgtggctg tctggaacag
3721 aaggagatgg tgagggtttt ggcagtgacc cctcagcttt ctcattgtgt gtcagttcat
3781 atgcatgctt gagttcatct ttaatgctgt tggactcttc tttgatgtag tccagctgct
3841 gcgttagtcg tctgacctca tgtctagctt cactcaggtg agtttcgagg gctttgattc
3901 tgctgtcctt gtctttagag tcagcctcgt gccgaattcc tgcagcccgg gggatccact
3961 agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt
4021 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
4081 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
4141 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
4201 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
4261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
4321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
4381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
4441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
4501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
4561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
4621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
4681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4861 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
4921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
5041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
5101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
5161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
5221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
5281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
5341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
5401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
5461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
5521 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
5581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
5641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
5701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
5761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
5821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
5881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
5941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
6001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
6061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
6121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
6181 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
6241 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
6301 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
6361 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
6421 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
6481 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
6541 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
6601 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc
6661 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
6721 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
6781 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
6841 attgggtacc gggccccccc tcgagttttt tttttttttt tttttttttt tttttgagta
6901 caaa
//
