| 质粒名称: | pXPR_502 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 96923 |
| 存储实验室: | John Doench, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pXPR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXPR_502 was a gift from John Doench & David Root (Addgene plasmid # 96923 ; http://n2t.net/addgene:96923 ; RRID:Addgene_96923) |
| 参考文献部分: | Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. 10.1038/s41467-018-07901-8 PubMed 30575746 |
买家导航
pXPR502载体基本信息
pXPR502载体图谱质粒图谱和多克隆位点信息
pXPR502载体简介
pXPR502载体序列
LOCUS pXPR_502 8694 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION for CRISPRa, lentiviral expression of gRNA scaffold using activator
P65 HSF.
ACCESSION .
VERSION .
KEYWORDS pXPR_502
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8694)
AUTHORS Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME,
Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG
TITLE Up, down, and out: next generation libraries for genome-wide
CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens
JOURNAL bioRxiv 356626; doi: https://doi.org/10.1101/356626
REFERENCE 2 (bases 1 to 8694)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8694
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 104..337
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
CDS complement(427..1023)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(507..527)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1004..1023
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
CDS complement(1051..1104)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS complement(1120..1491)
/codon_start=1
/product="C-terminal activation domain from the human heat
shock transcription factor HSF1"
/label=HSF1
/translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
PTISLLTGSEPPKAKDPTVS"
CDS complement(1516..2058)
/codon_start=1
/product="C-terminal portion of the p65 subunit of mouse
NF-kappa-B"
/label=p65
/translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
FSSIADMDFSALLSQISS"
CDS complement(2074..2094)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
protein_bind 2144..2168
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(2633..3133)
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(2696..2714)
/label=hPGK-F
/note="Human PGK promoter, forward primer"
misc_feature 3192..3309
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter complement(3591..3831)
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind complement(3641..3660)
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind complement(3811..3831)
/label=hU6-F
/note="Human U6 promoter, forward primer"
misc_feature 4238..4471
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4964..5089
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 5136..5316
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(5317..5543)
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
primer_bind complement(5571..5591)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5571..5589)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5610..5626)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5610..5626)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5623..5645)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5634..5650
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5658..5688)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5703..5724
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5841..5858)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6012..6600)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6092..6111)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6771..7631)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7394..7413
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7632..7736)
/gene="bla"
/label=AmpR promoter
rep_origin complement(7762..8217)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7899..7920)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 8111..8130
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8344..8366
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 8358..8375
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 8359..8375
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8385..8404
/label=T7
/note="T7 promoter, forward primer"
promoter 8385..8403
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 8424..8559
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(8478..8497)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 8599..26
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8610..8629)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 8664..8683
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
ORIGIN
1 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
61 tataaatact gaataataag atgacatgaa ctactatacg tactgctaga gattttccac
121 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
181 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
241 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc
301 agatcttgtc ttcgttggga gtgaattagc ccttccagtc cccccttttc ttttaaaaag
361 tggctaagat ctacagctgc cttgtaagtc attggtctta aagtcgactt aacgcgttca
421 gctagcggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
481 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
541 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
601 gctgcccaga cccttgccct ggtggtcggg cgaaacgccg acggtggcca ggaaccacgc
661 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
721 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
781 gctctccggc gtggtccaga ccgccacggc ggcgccgtcg tccgcgaccc acaccttgcc
841 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
901 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
961 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
1021 catggtaagc tccggtggat ctgtggtacc tgggccagga ttctcctcga cgtcaccgca
1081 tgttagcaga cttcctctgc cctctccact gccgatatcg gagacagtgg ggtccttggc
1141 tttgggaggc tccgagcctg tcagcaggga gatggtgggg tcctcggcga agccgtcccc
1201 ttcggagaag taggagccct ctcccagctc aaacagcacc ggcaggtcgt tgctcccggt
1261 gtccacggag ccggggtcca gcaggaacag cggctgcgct gtgtagtgca ccagctgctt
1321 ccctgaatcc gggctgctgt tctctgcctc gggaggcctg gggggctcct ggggagacag
1381 gagctcttgg atactggcca ggctgctgtc aaggtcaggc aggctcatgt cgggcacggt
1441 caccgagggg ctgaacaggt ccagcagggc actggtgtcc acgctgaagc cgcttccacc
1501 tcctccctgc ccactagagg aaatctgtga cagcagggca ctaaagtcca tatcagcgat
1561 gcttgagaag tcttcatctc cggacagccc attaggcagg ccgctggttc ccaggggagt
1621 tggagcgggg tcggggggcc gctggctgcc ggtcaccagc cgggtaatgg cttcggggta
1681 ctccatcagc attggttcgg ctgtactatg agacatggac acgccctgat tcagcagctg
1741 ctgaaactca gagttgtcca cggaggccag atctgtgaac actccgggat cggtgctgtt
1801 ccccagcaga gctcccaggt cctcatcagc gtcgaactgc aggtgcagca gagcttcact
1861 cagagtcccc tcgccggcct gtgtagactt gggcactgga gcgctcagtg actggggtgg
1921 tcctggggtc agcacagggg ctggagcagg tggctgggcc agaggcacca tagcactaga
1981 gggcaccata gtctgggcca gcactggagc ggagctaggg gccagagcca gggcctggtt
2041 gctgatctgc cctgaagggg taccagcggc cgccaccttc ctctttttct taggtccgct
2101 acctcctcct ccgcttcctc ctcctccgct tccacctcct ccgcccactt tgtacaagaa
2161 agttgaagaa cggcccagcg gcacaaggtt gacgacaaga tcttcgacct gcgaggtcgc
2221 gacgagggac ttggtcaaat cgtacaacga tttgcgcgag gcctcggtgc tattcgcaac
2281 gattgtcacg tcgtgcgacc atacctgagt gtagcgcact ttcggaagtc cggaatcaac
2341 gacgtccgcc tgatccagtt ttaggttgac tcgatacgcg gtcttggctc cgttttgacg
2401 gagcgaagcc gtgaggcgca gccgacccac cagaggcccg accttctctt cgaagatctg
2461 acggtctgcg gtggactgga tctcagtcag agtgcgagta gcctcgccga ccgaaagaac
2521 gatggttttg gaggctagca tagacgtctt cctccttttg tgaggttgtc caccatcgtt
2581 ctcgtagctg tgccgcctcc gcgacatggt ggcaagctta agttggatcc ccctggggag
2641 agaggtcggt gattcggtca acgagggagc cgactgccga cgtgcgctcc ggaggcttgc
2701 agaatgcgga acaccgcgcg ggcaggaaca gggcccacac taccgcccca caccccgcct
2761 cccgcaccgc cccttcccgg ccgctgctct cggcgcgccc tgctgagcag ccgctattgg
2821 ccacagccca tcgcggtcgg cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg
2881 tactagtaag acgtgcggct tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac
2941 cttcccgact taggggcgga gcaggaagcg tcgccggggg gcccacaagg gtagcggcga
3001 agatccgggt gacgctgcga acggacgtga agaatgtgcg agacccaggg tcggcgccgc
3061 tgcgtttccc ggaaccacgc ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt
3121 ggaaaaggcg caacccgggc cccctcgagc cggcgccaaa gtggatctct gctgtccctg
3181 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
3241 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
3301 cttttaaaat tgtggatgaa tactgccatt tgtctcaaga tctagaattc aaaaaaagca
3361 ccgactcggt gccacttttt caagttgata acggactagc cttatttaaa cttgctatgc
3421 tgcgaatacg agcagaccct gatggtgtct gatacgtacc ctgatggtgt acgagaccac
3481 ggagcccata tgctctcgtg gagaggagcg acgccatatc gtctgctccc tcgtattcgc
3541 agcatagctc ttaaacagag acgcaaaaaa ccggtcgtct ccggtgtttc gtcctttcca
3601 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
3661 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
3721 cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta tctctctaac
3781 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct ctctgggtcc
3841 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
3901 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
3961 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
4021 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
4081 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
4141 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
4201 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
4261 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
4321 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
4381 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
4441 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
4501 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
4561 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
4621 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
4681 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
4741 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
4801 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
4861 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
4921 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
4981 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
5041 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
5101 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
5161 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
5221 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
5281 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
5341 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
5401 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
5461 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
5521 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
5581 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
5641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
5701 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
5761 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
5821 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
5881 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
5941 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
6001 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
6061 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
6121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
6181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
6241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
6301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
6361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
6421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
6481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
6541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
6601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
6661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
6721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
6781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
6841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
6901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
6961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
7021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
7081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
7141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
7201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
7261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
7321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
7381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
7441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
7501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
7561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
7621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
7681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
7741 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
7801 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
7861 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
7921 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
7981 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
8041 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
8101 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
8161 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
8221 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
8281 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
8341 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
8401 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
8461 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
8521 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat
8581 agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
8641 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaa
//
