| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET16b, pet 16b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5711bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-Factor Xa蛋白酶切位点 |
| 载体抗性: | Ampicillin |
| 备注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 产品目录号: | 69662-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pet16b载体基本信息
pet16b载体图谱质粒图谱和多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 316 | 188 | |
| T7_Terminal_primer | 256 | 274 | |
| T7_transl_en_RBS | 419 | 403 | |
| lacO | 464 | 437 | |
| T7_promoter | 482 | 464 | |
| pBRrevBam_primer | 585 | 566 | |
| lacI | 860 | 1951 | |
| tet (576 - 1190) | 2010 | 2624 | |
| ROP | 3263 | 3454 | |
| pGEX_3_primer | 3470 | 3448 | |
| Ampicillin | 5503 | 4643 | |
| AmpR_promoter | 5573 | 5545 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 619 | 1119 | |
| ORF frame 2 | 992 | 1951 | |
| ORF frame 1 | 2429 | 1734 | |
| ORF frame 3 | 2007 | 2624 | |
| ORF frame 2 | 5503 | 4643 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| BamHI | 319 |
| XhoI | 324 |
| NdeI | 331 |
| NcoI | 392 |
| XbaI | 431 |
| BglII | 497 |
| BclI | 1233 |
| ApaI | 1430 |
| HpaI | 1725 |
| EagI | 2287 |
| NruI | 2322 |
| MscI | 2794 |
| PstI | 4961 |
| AatII | 5638 |
| EcoRI | 5709 |
pet16b载体简介
pET-16b载体具有N端His标签和Factor Xa酶切位点。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pet16b载体序列
LOCUS pET-16b 5711 bp DNA circular SYN
DEFINITION pET-16b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5711
/organism="pET-16b"
/mol_type="other DNA"
terminator complement(188..316)
/label="T7_terminator"
misc_feature 256..274
/label="T7_Terminal_primer"
misc_feature complement(403..419)
/label="T7_transl_en_RBS"
misc_feature complement(437..464)
/label="lacO"
promoter complement(464..482)
/label="T7_promoter"
misc_feature complement(566..585)
/label="pBRrevBam_primer"
CDS 619..1119
/label="ORF frame 1"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
misc_feature 860..1951
/label="lacI"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
CDS 992..1951
/label="ORF frame 2"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(1734..2429)
/label="ORF frame 1"
/translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL
TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS
GPRWFAPAGENPV*"
CDS 2007..2624
/label="ORF frame 3"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
gene 2010..2624
/label="tet (576 - 1190)"
/gene="tet (576 - 1190)"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
misc_feature 3263..3454
/label="ROP"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
misc_feature complement(3448..3470)
/label="pGEX_3_primer"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
gene complement(4643..5503)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
CDS complement(4643..5503)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5545..5573)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
301 cgggctttgt tagcagccgg atcctcgagc atatgacgac cttcgatatg gccgctgctg
361 tgatgatgat gatgatgatg atgatgatgg cccatggtat atctccttct taaagttaaa
421 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
481 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
541 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
601 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
661 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
721 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
781 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
841 ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
901 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
961 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
1021 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
1081 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
1141 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
1201 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
1261 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
1321 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
1381 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
1441 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
1501 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
1561 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
1621 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
1681 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
1741 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
1801 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
1861 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
1921 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca
1981 ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
2041 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
2101 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
2161 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
2221 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
2281 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
2341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
2401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
2461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
2521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
2581 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
2641 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
2701 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
2761 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
2821 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
2881 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
2941 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
3001 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
3061 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
3121 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
3181 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
3241 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
3301 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
3361 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
3421 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
3481 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
3541 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
3601 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
3661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
4321 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
5641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
5701 gtcttcaaga a
//
