| 质粒名称: | pHAGE-EFS-PCP-3XGFPnls |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75385 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE-DEST |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6000 bp |
| 完整质粒大小: | 8758 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PCP |
| 物种来源: | Synthetic |
| 插入大小: | 369 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EFS |
| 标签/融合蛋白: | 3XsfGFP（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Nco I(未破坏) |
| 3' 克隆位点: | BamH I (未破坏) |
| 5' 测序引物 | cgaaggaatagaagaagaaggtgga |
| 3' 测序引物 | CCAAAGGGAGATCCGACTCG |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE-EFS-PCP-3XGFPnls was a gift from Thoru Pederson (Addgene plasmid # 75385 ; http://n2t.net/addgene:75385 ; RRID:Addgene_75385) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHAGE-EFS-PCP-3XGFPnls 8758 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION PCP-3XGFPnls.
ACCESSION .
VERSION .
KEYWORDS pHAGE-EFS-PCP-3XGFPnls
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8758)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8758)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8758
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2265..2476
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 2522..2531
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2933..3646
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
CDS 3665..4378
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
CDS 4397..5110
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
CDS 5117..5137
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 5148..5736
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5201..5221)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5619..5630)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5811..6044
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6163..6181)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6249..6353
/gene="bla"
/label=AmpR promoter
CDS 6354..7214
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6572..6591)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7385..7973
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7874..7893
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8127..8144
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8236..8255
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8384..8405
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8420..8450
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8458..8474
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8463..8485
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8482..8498
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8482..8498
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8548..8567
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttgacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagcgat
2221 gctagctgat gcgggccctt cgagtggctc cggtgcccgt cagtgggcag agcgcacatc
2281 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
2341 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
2401 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
2461 tgccgccaga acacaggtgt cgtgacgcgg ttccggttaa ggtgaacgcg tgcaggctgg
2521 cgccaccatg ggttccaaaa ccatcgttct ttcggtcggc gaggctactc gcactctgac
2581 tgagatccag tccaccgcag accgtcagat cttcgaagag aaggtcgggc ctctggtggg
2641 tcggctgcgc ctcacggctt cgctccgtca aaacggagcc aagaccgcgt atcgcgtcaa
2701 cctaaaactg gatcaggcgg acgtcgttga ttccggactt ccgaaagtgc gctacactca
2761 ggtatggtcg cacgacgtga caatcgttgc gaatagcacc gaggcctcgc gcaaatcgtt
2821 gtacgatttg accaagtccc tcgtcgcgac ctcgcaggtc gaagatcttg tcgtcaacct
2881 tgtgccgctg ggccgtggtg gcggagggac tagtggaggt ggatccggaa gtatgcgtaa
2941 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
3001 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
3061 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
3121 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
3181 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
3241 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
3301 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
3361 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
3421 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
3481 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
3541 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
3601 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatctg gcggccgctc
3661 tggcatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga
3721 tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa
3781 tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac
3841 tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa
3901 gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc
3961 ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct
4021 ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca
4081 taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa
4141 tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc
4201 tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca
4261 ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt
4321 tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaagg
4381 tagcatcgat ggtagcatgc gtaaaggcga agagctgttc actggtgtcg tccctattct
4441 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
4501 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
4561 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
4621 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
4681 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
4741 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa
4801 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
4861 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
4921 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
4981 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
5041 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
5101 actgtacaaa ctcgagccaa agaaaaagcg gaaagtgtaa tctagataat caacctctgg
5161 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
5221 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
5281 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
5341 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
5401 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
5461 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
5521 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
5581 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
5641 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
5701 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
5761 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
5821 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
5881 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
5941 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
6001 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
6061 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
6121 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
6181 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
6241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
6301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
6361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
6421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
6481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
6541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
6601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
6661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
6721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
6781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
6841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
6901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
6961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
7021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
7081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
7141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
7201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
7261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
7321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
7381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
7441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
7501 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
7561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
7621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
7681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
7741 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
7801 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
7861 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
7921 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
7981 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
8041 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
8101 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
8161 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
8221 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
8281 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
8341 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
8401 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
8461 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
8521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
8581 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
8641 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
8701 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
