质粒名称: | pWPXLd |
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出品公司: | 爱迪基因 |
目录编号: | 12258 |
存储实验室: | Didier Trono |
载体骨架基本信息 |
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载体名称: | pWPXLd |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | 10455 bp |
载体修饰: | -- |
载体类型: | 哺乳动物表达,慢病毒 |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | EGFP |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | MCS - see map (未销毁) |
3' 克隆位点: | MCS - see map (未销毁) |
5' 测序引物 | See map |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pWPXLd was a gift from Didier Trono (Addgene plasmid # 12258 ; http://n2t.net/addgene:12258 ; RRID:Addgene_12258) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pWPXLd 10455 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pWPXLd SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10455) TITLE Trono Lab Constitutive Lentiviral Plasmids REFERENCE 2 (bases 1 to 10455) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10455 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 2..635 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 682..807 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1300..1533 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." promoter 2120..3297 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2351..3288 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3245..3265 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 3345..3462 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" regulatory 3538..3547 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3544..4260 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3589..3610) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3850..3869) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4197..4218 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4340..4928 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4393..4413) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4811..4822) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5017..5250 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(5275..5293) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5276..5293) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(5583..5602) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(5816..5835) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5935..5957 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5995..6013) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6081..6185 /gene="bla" /label=AmpR promoter CDS 6186..7046 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6404..6423) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7217..7805 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7706..7725 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7959..7976 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(8046..8066) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8051..8380 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8231..8366 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8293..8312 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 9514..9579 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 9709..9729 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 10154..10288 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10191..10210) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10245..10264 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt 2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt 2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg 2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg 2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt 2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga 3301 atttcgacat ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg 3361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 3421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttccgatcac gagactagcc 3481 tcgaggttta aactacggga tccaggccta agcttacgcg tcctagcgct accggtcgcc 3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4261 tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa cttcgaattc 4321 ccgggactag tcatatgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 4381 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 4441 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 4501 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 4561 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 4621 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 4681 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 4741 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 4801 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 4861 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4921 ctccccgcat cggtacgtat ggccaggtac ctttaagacc aatgacttac aaggcagctg 4981 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 5041 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 5101 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5161 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5221 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata 5281 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc 5341 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc 5401 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg 5461 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca 5521 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg 5581 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag 5641 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat 5701 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg 5761 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa 5821 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 5881 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 5941 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 6001 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 6061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 6121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 6181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 6241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 6301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 6361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 6421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 6481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 6541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 6601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 6661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 6721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 6781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 6841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 7021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 7081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 7141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 7201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 7261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 7321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 7381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 7441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 7501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 7561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg 7621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 7681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 7741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 7801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 7861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 7921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 7981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 8041 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 8101 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 8161 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 8221 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 8281 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 8341 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg 8401 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa 8461 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc 8521 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca 8581 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa 8641 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt 8701 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc 8761 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg 8821 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg 8881 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct 8941 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat 9001 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc 9061 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg 9121 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc 9181 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg 9241 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct 9301 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg 9361 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga 9421 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg 9481 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa 9541 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat 9601 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg 9661 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag 9721 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct 9781 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca 9841 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt 9901 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat 9961 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa 10021 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt 10081 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 10141 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 10201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 10261 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc 10321 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct 10381 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 10441 ctacaaactt agtag //
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