质粒名称: | pAdDeltaF6 |
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出品公司: | 爱迪基因 |
目录编号: | 112867 |
存储实验室: | James M. Wilson |
载体骨架基本信息 |
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载体名称: | -- |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | AAV |
筛选标记: | -- |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | E4, E2a and VA |
物种来源: | 腺病毒 |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pAdDeltaF6 was a gift from James M. Wilson (Addgene plasmid # 112867 ; http://n2t.net/addgene:112867 ; RRID:Addgene_112867) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAdDeltaF6 15420 bp ds-DNA circular SYN 17-MAY-2019 DEFINITION AAV helper plasmid. ACCESSION . VERSION . KEYWORDS pAdDeltaF6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15420) TITLE AAV packaging plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 15420) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15420 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..62) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(46..62) /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(99..119) /label=T3 /note="T3 promoter, forward primer" promoter complement(99..117) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(138..154) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(138..154) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(151..173) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 162..178 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(186..216) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 231..252 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(369..386) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(540..1128) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(620..639) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1299..2159) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1922..1941 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2160..2264) /gene="bla" /label=AmpR promoter primer_bind 2332..2350 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2377..2405 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" ORIGIN 1 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta 61 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg 121 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 181 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 241 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 301 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 961 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 1021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 1141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 1201 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 1261 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 1321 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 1381 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 1441 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 1501 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 1561 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 1621 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 1681 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 1741 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 1801 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 1861 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 1921 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 1981 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 2041 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 2101 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 2161 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 2221 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 2281 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 2341 atcacgaggc cctttcgtct tcaagaattc tcatgtttga cagcttatca tcgataagct 2401 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc 2461 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt 2521 ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag 2581 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct 2641 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc 2701 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatccggc gcacaccaaa 2761 aacgtcactt ttgccacatc cgtcgcttac atgtgttccg ccacacttgc aacatcacac 2821 ttccgccaca ctactacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc 2881 caccccctca ttatcatatt ggcttcaatc caaaataatc atcaataata taccttattt 2941 tggattgaag ccaatatgat aatgaggggg tggagtttgt gacgtggcgc ggggcgtggg 3001 aacggggcgg gtgacgtagg ttttagggcg gagtaacttg tatgtgttgg gaattgtagt 3061 tttcttaaaa tgggaagtga cgtaacgtgg gaaaacggaa gtgacgattt gaggaagttg 3121 tgggtttttt ggctttcgtt tctgggcgta ggttcgcgtg cggttttctg ggtgtttttt 3181 gtggacttta accgttacgt cattttttag tcctatatat actcgctctg cacttggccc 3241 ttttttacac tgtgactgat tgagctggtg ccgtgtcgag tggtgttttt ttaataggtt 3301 ttctttttta ctggtaaggc tgactgttat ggctgccgct gtggaagcgc tgtatgttgt 3361 tctggagcgg gagggtgcta ttttgcctag gcaggagggt ttttcaggtg tttatgtgtt 3421 tttctctcct attaattttg ttatacctcc tatgggggct gtaatgttgt ctctacgcct 3481 gcgggtatgt attcccccgg gctatttcgg tcgcttttta gcactgaccg atgtgaatca 3541 acctgatgtg tttaccgagt cttacattat gactccggac atgaccgagg agctgtcggt 3601 ggtgcttttt aatcacggtg accagttttt ttacggtcac gccggcatgg ccgtagtccg 3661 tcttatgctt ataagggttg tttttcctgt tgtaagacag gcttctaatg tttaaatgtt 3721 tttttgttat tttattttgt gtttatgcag aaacccgcag acatgtttga gagaaaaatg 3781 gtgtcttttt ctgtggtggt tccggagctt acctgccttt atctgcatga gcatgactac 3841 gatgtgcttt cttttttgcg cgaggctttg cctgattttt tgagcagcac cttgcatttt 3901 atatcgccgc ccatgcaaca agcttacatc ggggctacgc tggttagcat agctccgagt 3961 atgcgtgtca taatcagtgt gggttctttt gtcatggttc ctggcgggga agtggccgcg 4021 ctggtccgtg cagacctgca cgattatgtt cagctggccc tgcgaaggga cctacgggat 4081 cgcggtattt ttgttaatgt tccgcttttg aatcttatac aggtctgtga ggaacctgaa 4141 tttttgcaat catgattcgc tgcttgaggc tgaaggtgga gggcgctctg gagcagattt 4201 ttacaatggc cggacttaat attcgggatt tgcttagaga tatattgaga aggtggcgag 4261 atgagaatta tttgggcatg gttgaaggtg ctggaatgtt tatagaggag attcaccctg 4321 aagggtttag cctttacgtc cacttggacg tgagggccgt ttgccttttg gaagccattg 4381 tgcaacatct tacaaatgcc attatctgtt ctttggctgt agagtttgac cacgccaccg 4441 gaggggagcg cgttcactta atagatcttc attttgaggt tttggataat cttttggaat 4501 aaaaaaaaaa acatggttct tccagctctt cccgctcctc ccgtgtgtga ctcgcagaac 4561 gaatgtgtag gttggctggg tgtggcttat tctgcggtgg tggatgttat cagggcagcg 4621 gcgcatgaag gagtttacat agaacccgaa gccagggggc gcctggatgc tttgagagag 4681 tggatatact acaactacta cacagagcga tctaagcggc gagaccggag acgcagatct 4741 gtttgtcacg cccgcacctg gttttgcttc aggaaatatg actacgtccg gcgttccatt 4801 tggcatgaca ctacgaccaa cacgatctcg gttgtctcgg cgcactccgt acagtaggga 4861 tcgtctacct ccttttgaga cagaaacccg cgctaccata ctggaggatc atccgctgct 4921 gcccgaatgt aacactttga caatgcacaa cgtgagttac gtgcgaggtc ttccctgcag 4981 tgtgggattt acgctgattc aggaatgggt tgttccctgg gatatggttc taacgcggga 5041 ggagcttgta atcctgagga agtgtatgca cgtgtgcctg tgttgtgcca acattgatat 5101 catgacgagc atgatgatcc atggttacga gtcctgggct ctccactgtc attgttccag 5161 tcccggttcc ctgcagtgta tagccggcgg gcaggttttg gccagctggt ttaggatggt 5221 ggtggatggc gccatgttta atcagaggtt tatatggtac cgggaggtgg tgaattacaa 5281 catgccaaaa gaggtaatgt ttatgtccag cgtgtttatg aggggtcgcc acttaatcta 5341 cctgcgcttg tggtatgatg gccacgtggg ttctgtggtc cccgccatga gctttggata 5401 cagcgccttg cactgtggga ttttgaacaa tattgtggtg ctgtgctgca gttactgtgc 5461 tgatttaagt gagatcaggg tgcgctgctg tgcccggagg acaaggcgcc ttatgctgcg 5521 ggcggtgcga atcatcgctg aggagaccac tgccatgttg tattcctgca ggacggagcg 5581 gcggcggcag cagtttattc gcgcgctgct gcagcaccac cgccctatcc tgatgcacga 5641 ttatgactct acccccatgt aggcgtggac ttctccttcg ccgcccgtta agcaaccgca 5701 agttggacag cagcctgtgg ctcagcagct ggacagcgac atgaacttaa gtgagctgcc 5761 cggggagttt attaatatca ctgatgagcg tttggctcga caggaaaccg tgtggaatat 5821 aacacctaag aatatgtctg ttacccatga tatgatgctt tttaaggcca gccggggaga 5881 aaggactgtg tactctgtgt gttgggaggg aggtggcagg ttgaatacta gggttctgtg 5941 agtttgatta aggtacggtg atctgtataa gctatgtggt ggtggggcta tactactgaa 6001 tgaaaaatga cttgaaattt tctgcaattg aaaaataaac acgttgaaac ataacacaaa 6061 cgattcttta ttcttgggca atgtatgaaa aagtgtaaga ggatgtggca aatatttcat 6121 taatgtagtt gtggccagac cagtcccatg aaaatgacat agagtatgca cttggagttg 6181 tgtctcctgt ttcctgtgta ccgtttagtg taatggttag tgttacaggt ttagttttgt 6241 ctccgtttaa gtaaacttga ctgacaatgt tacttttggc agttttaccg tgagattttg 6301 gataagctga taggttaggc ataaatccaa cagcgtttgt ataggctgtg ccttcagtaa 6361 gatctccatt tctaaagttc caatattctg ggtccaggaa ggaattgttt agtagcactc 6421 cattttcgtc aaatcttata ataagatgag cactttgaac tgttccagat attggagcca 6481 aactgccttt aacagccaaa actgaaactg tagcaagtat ttgactgcca cattttgtta 6541 agaccaaagt gagtttagca tctttctctg catttagtct acagttagga gatggagctg 6601 gtgtggtcca caaagttagc ttatcattat ttttgtttcc tactgtaatg gcacctgtgc 6661 tgtcaaaact aaggccagtt cctagtttag gaaccatagc cttgtttgaa tcaaattcta 6721 ggccatggcc aatttttgtt ttgaggggat ttgtgtttgg tgcattaggt gaaccaaatt 6781 caagcccatc tcctgcatta atggctatgg ctgtagcgtc aaacatcaac cccttggcag 6841 tgcttaggtt aacctcaagc tttttggaat tgtttgaagc tgtaaacaag taaaggcctt 6901 tgttgtagtt aatatccaag ttgtgggctg agtttataaa aagagggccc tgtcctagtc 6961 ttagatttag ttggttttga gcatcaaacg gataactaac atcaagtata aggcgtctgt 7021 tttgagaatc aatccttagt cctcctgcta cattaagttg catattgcct tgtgaatcaa 7081 aacccaaggc tccagtaact ttagtttgca aggaagtatt attaatagtc acacctggac 7141 cagttgctac ggtcaaagtg tttaggtcgt ctgttacatg caaaggagcc ccgtacttta 7201 gtcctagttt tccattttgt gtataaatgg gctctttcaa gtcaatgccc aagctaccag 7261 tggcagtagt tagagggggt gaggcagtga tagtaagggt actgctatcg gtggtggtga 7321 gggggcctga tgtttgcagg gctagctttc cttctgacac tgtgaggggt ccttgggtgg 7381 caatgctaag tttggagtcg tgcacggtta gcggggcctg tgattgcatg gtgagtgtgt 7441 tgcccgcgac cattagaggt gcggcggcag ccacagttag ggcttctgag gtaactgtga 7501 ggggtgcaga tatttccagg tttatgtttg acttggtttt tttgagaggt gggctcacag 7561 tggttacatt ttgggaggta aggttgccgg cctcgtccag agagaggccg ttgcccattt 7621 tgagcgcaag catgccattg gaggtaacta gaggttcgga taggcgcaaa gagagtaccc 7681 cagggggact ctcttgaaac ccattggggg atacaaaggg aggagtaaga aaaggcacag 7741 ttggaggacc ggtttccgtg tcatatggat acacggggtt gaaggtatct tcagacggtc 7801 ttgcgcgctt catctgcaac aacatgaaga tagtgggtgc ggatggacag gaacaggagg 7861 aaactgacat tccatttaga ttgtggagaa agtttgcagc caggaggaag ctgcaatacc 7921 agagctggga ggagggcaag gaggtgctgc tgaataaact ggacagaaat ttgctaactg 7981 attttaagta agtgatgctt tattattttt ttttattagt taaagggaat aagatccccg 8041 ggtactctag ttaattaact agaggatctt gatgtaatcc agggttagga cagttgcaaa 8101 tcacagtgag aacacagggt cccctgtccc gctcaactag cagggggcgc tgggtaaact 8161 cccgaatcag gctacgggca agctctccct gggcggtaag ccggacgccg tgcgccgggc 8221 cctcgatatg atcctcgggc aattcaaagt agcaaaactc accggagtcg cgggcaaagc 8281 acttgtggcg gcgacagtgg accaggtgtt tcaggcgcag ttgctctgcc tctccactta 8341 acattcagtc gtagccgtcc gccgagtcct ttaccgcgtc aaagttagga ataaattgat 8401 ccggatagtg gccgggaggt cccgagaagg ggttaaagta gaccgatggc acaaactcct 8461 caataaattg cagagttcca atgcctccag agcgcggctc agaggacgag gtctgcagag 8521 ttaggattgc ctgacgaggc gtgaatgaag agcggccggc gccgccgatc tgaaatgtcc 8581 cgtccggacg gagaccaagc gaggagctca ccgactcgtc gttgagctga atacctcgcc 8641 ctctgattgt caggtgagtt ataccctgcc cgggcgaccg caccctgtga cgaaagccgc 8701 ccgcaagctg cgcccctgag ttagtcatct gaacttcggc ctgggcgtct ctgggaagta 8761 ccacagtggt gggagcggga ctttcctggt acaccagggc agcgggccaa ctacggggat 8821 taaggttatt acgaggtgtg gtggtaatag ccgcctgttc caggagaatt cggtttcggt 8881 gggcgcgtat tccgttgacc cgggatatca tgtggggtcc cgcgctcatg tagtttattc 8941 gggttgagta gtcttgggca gctccagccg caagtcccat ttgtggctgg taactccaca 9001 tgtagggcgt gggaatttcc ttgctcataa tggcgctgac aacaggtgct ggcgccgggt 9061 gtggccgctg gagatgacgt agttttcgcg cttaaatttg agaaagggcg cgaaactagt 9121 ccttaagagt cagcgcgcag tatttactga agagagcctc cgcgtcttcc agcgtgcgcc 9181 gaagctgatc ttcgcttttg tgatacaggc agctgcgggt gagggatcgc agagacctgt 9241 tttttatttt cagctcttgt tcttggcccc tgctctgttg aaatatagca tacagagtgg 9301 gaaaaatcct gtttctaagc tcgcgggtcg atacgggttc gttgggcgcc agacgcagcg 9361 ctcctcctcc tgctgctgcc gccgctgtgg atttcttggg ctttgtcaga gtcttgctat 9421 ccggtcgcct ttgcttctgt gtggccgctg ctgttgctgc cgctgccgcc ggtgcagtat 9481 gggctgtaga gatgacggta gtaatgcagg atgttacggg ggaaggccac gccgtgatgg 9541 tagagaagaa agcggcgggc gaaggagatg ttgcccccac agtcttgcaa gcaagcaact 9601 atggcgttct tgtgcccgcg ccatgagcgg tagccttggc gctgttgttg ctcttgggct 9661 aacggcggcg gctgcttgga cttaccggcc ctggttccag tggtgtccca tctacggttg 9721 ggtcggcgaa cgggcagtgc cggcggcgcc tgaggagcgg aggttgtagc catgctggaa 9781 ccggttgccg atttctgggg cgccggcgag gggaatgcga ccgagggtga cggtgtttcg 9841 tctgacacct cttcgacctc ggaagcttcc tcgtctaggc tctcccagtc ttccatcatg 9901 tcctcctcct cctcgtccaa aacctcctct gcctgactgt cccagtattc ctcctcgtcc 9961 gtgggtggcg gcggcagctg cagcttcttt ttgggtgcca tcctgggaag caagggcccg 10021 cggctgctgc tgatagggct gcggcggcgg ggggattggg ttgagctcct cgccggactg 10081 ggggtccaag taaacccccc gtccctttcg tagcagaaac tcttggcggg ctttgttgat 10141 ggcttgcaat tggccaagaa tgtggccctg ggtaatgacg caggcggtaa gctccgcatt 10201 aggcgggcgg gattggtctt cgtagaacct aatctcgtgg gcgtggtagt cctcaggtac 10261 aaatttgcga aggtaagccg acgtccacag ccccggagtg agtttcaacc ccggagccgc 10321 ggacttttcg tcaggcgagg gaccctgcag ctcaaaggta ccgataattt gactttcgtt 10381 aagcagctgc gaattgcaaa ccagggagcg gtgcggggtg cataggttgc agcgacagtg 10441 acactccagt agaccgtcac cgctcacgtc ttccattatg tcagagtggt aggcaaggta 10501 gttggctagc tgcagaaggt agcagtggcc ccaaagcggc ggagggcatt cgcggtactt 10561 aatgggcaca aagtcgctag gaagtgcaca gcaggtggcg ggcaagattc ctgagcgctc 10621 taggataaag ttcctaaagt tctgcaacat gctttgactg gtgaagtctg gcagaccctg 10681 ttgcagggtt ttaagcaggc gttcggggaa aatgatgtcc gccaggtgcg cggccacgga 10741 gcgctcgttg aaggccgtcc ataggtcctt caagttttgc tttagcagtt tctgcagctc 10801 cttgaggttg cactcctcca agcactgctg ccaaacgccc atggccgtct gccaggtgta 10861 gcatagaaat aagtaaacgc agtcgcggac gtagtcgcgg cgcgcctcgc ccttgagcgt 10921 ggaatgaagc acgttttgcc caaggcggtt ttcgtgcaaa attccaaggt aggagaccag 10981 gttgcagagc tccacgttgg agatcttgca ggcctggcgt acgtagccct gtcgaaaggt 11041 gtagtgcaat gtttcctcta gcttgcgctg catctccggg tcagcaaaga accgctgcat 11101 gcactcaagc tccacggtaa cgagcactgc ggccatcatt agtttgcgtc gctcctccaa 11161 gtcggcaggc tcgcgcgttt gaagccagcg cgctagctgc tcgtcgccaa ctgcgggtag 11221 gccctcctct gtttgttctt gcaaatttgc atccctctcc aggggctgcg cacggcgcac 11281 gatcagctca ctcatgactg tgctcatgac cttggggggt aggttaagtg ccgggtaggc 11341 aaagtgggtg acctcgatgc tgcgttttag tacggctagg cgcgcgttgt caccctcgag 11401 ttccaccaac actccagagt gactttcatt ttcgctgttt tcctgttgca gagcgtttgc 11461 cgcgcgcttc tcgtcgcgtc caagaccctc aaagattttt ggcacttcgt tgagcgaggc 11521 gatatcaggt atgacagcgc cctgccgcaa ggccagctgc ttgtccgctc ggctgcggtt 11581 ggcacggcag gataggggta tcttgcagtt ttggaaaaag atgtgatagg tggcaagcac 11641 ctctggcacg gcaaatacgg ggtagaagtt gaggcgcggg ttgggctcgc atgtgccgtt 11701 ttcttggcgt ttggggggta cgcgcggtga gaataggtgg cgttcgtagg caaggctgac 11761 atccgctatg gcgaggggca catcgctgcg ctcttgcaac gcgtcgcaga taatggcgca 11821 ctggcgctgc agatgcttca acagcacgtc gtctcccaca tctaggtagt cgccatgcct 11881 ttcgtccccc cgcccgactt gttcctcgtt tgcctctgcg ttgtcctggt cttgcttttt 11941 atcctctgtt ggtactgagc ggtcctcgtc gtcttcgctt acaaaacctg ggtcctgctc 12001 gataatcact tcctcctcct caagcggggg tgcctcgacg gggaaggtgg taggcgcgtt 12061 ggcggcatcg gtggaggcgg tggtggcgaa ctcagagggg gcggttaggc tgtccttctt 12121 ctcgactgac tccatgatct ttttctgcct ataggagaag gaaatggcca gtcgggaaga 12181 ggagcagcgc gaaaccaccc ccgagcgcgg acgcggtgcg gcgcgacgtc ccccaaccat 12241 ggaggacgtg tcgtccccgt ccccgtcgcc gccgcctccc cgggcgcccc caaaaaagcg 12301 gatgaggcgg cgtatcgagt ccgaggacga ggaagactca tcacaagacg cgctggtgcc 12361 gcgcacaccc agcccgcggc catcgacctc ggcggcggat ttggccattg cgcccaagaa 12421 gaaaaagaag cgcccttctc ccaagcccga gcgcccgcca tcaccagagg taatcgtgga 12481 cagcgaggaa gaaagagaag atgtggcgct acaaatggtg ggtttcagca acccaccggt 12541 gctaatcaag catggcaaag gaggtaagcg cacagtgcgg cggctgaatg aagacgaccc 12601 agtggcgcgt ggtatgcgga cgcaagagga agaggaagag cccagcgaag cggaaagtga 12661 aattacggtg atgaacccgc tgagtgtgcc gatcgtgtct gcgtgggaga agggcatgga 12721 ggctgcgcgc gcgctgatgg acaagtacca cgtggataac gatctaaagg cgaacttcaa 12781 actactgcct gaccaagtgg aagctctggc ggccgtatgc aagacctggc tgaacgagga 12841 gcaccgcggg ttgcagctga ccttcaccag caacaagacc tttgtgacga tgatggggcg 12901 attcctgcag gcgtacctgc agtcgtttgc agaggtgacc tacaagcatc acgagcccac 12961 gggctgcgcg ttgtggctgc accgctgcgc tgagatcgaa ggcgagctta agtgtctaca 13021 cggaagcatt atgataaata aggagcacgt gattgaaatg gatgtgacga gcgaaaacgg 13081 gcagcgcgcg ctgaaggagc agtctagcaa ggccaagatc gtgaagaacc ggtggggccg 13141 aaatgtggtg cagatctcca acaccgacgc aaggtgctgc gtgcacgacg cggcctgtcc 13201 ggccaatcag ttttccggca agtcttgcgg catgttcttc tctgaaggcg caaaggctca 13261 ggtggctttt aagcagatca aggcttttat gcaggcgctg tatcctaacg cccagaccgg 13321 gcacggtcac cttttgatgc cactacggtg cgagtgcaac tcaaagcctg ggcacgcgcc 13381 ctttttggga aggcagctac caaagttgac tccgttcgcc ctgagcaacg cggaggacct 13441 ggacgcggat ctgatctccg acaagagcgt gctggccagc gtgcaccacc cggcgctgat 13501 agtgttccag tgctgcaacc ctgtgtatcg caactcgcgc gcgcagggcg gaggccccaa 13561 ctgcgacttc aagatatcgg cgcccgacct gctaaacgcg ttggtgatgg tgcgcagcct 13621 gtggagtgaa aacttcaccg agctgccgcg gatggttgtg cctgagttta agtggagcac 13681 taaacaccag tatcgcaacg tgtccctgcc agtggcgcat agcgatgcgc ggcagaaccc 13741 ctttgatttt taaacggcgc agacggcaag ggtgggggta aataatcacc cgagagtgta 13801 caaataaaag catttgcctt tattgaaagt gtctctagta cattattttt acatgttttt 13861 caagtgacaa aaagaagtgg cgctcctaat ctgcgcactg tggctgcgga agtagggcga 13921 gtggcgctcc aggaagctgt agagctgttc ctggttgcga cgcagggtgg gctgtacctg 13981 gggactgttg agcatggagt tgggtacccc ggtaataagg ttcatggtgg ggttgtgatc 14041 catgggagtt tggggccagt tggcaaaggc gtggagaaac atgcagcaga atagtccaca 14101 ggcggccgag ttgggcccct gtacgctttg ggtggacttt tccagcgtta tacagcggtc 14161 gggggaagaa gcaatggcgc tacggcgcag gagtgactcg tactcaaact ggtaaacctg 14221 cttgagtcgc tggtcagaaa agccaaaggg ctcaaagagg tagcatgttt ttgagtgcgg 14281 gttccaggca aaggccatcc agtgtacgcc cccagtctcg gtccgagact cgaaccgggg 14341 gtcccgcgac tcaacccttg gaaaataacc ctccggctac agggagcgag ccacttaatg 14401 ctttcgcttt ccagcctaac cgcttacgct gcgcgcggcc agtggccaaa aaagctagcg 14461 cagcagccgc cgcgcctgga aggaagccaa aaggagcact cccccgttgt ctgacgtcgc 14521 acacctgggt tcgacacgcg ggcggtaacc gcatggatca cggcggacgg ccggatacgg 14581 ggctcgaacc ccggtcgtcc gccatgatac ccttgcgaat ttatccacca gaccacggaa 14641 gagtgcccgc ttacaggctc tccttttgca cgctagagcg tcaacgattg cgcgcgcctg 14701 accggccaga gcgtcccgac catggagcac tttttgccgc tgcgcaacat ctggaaccgc 14761 gtccgcgact ttccgcgcgc ctccaccacc gccgccggca tcacctggat gtccaggtac 14821 atctacggat atcatcgcct tatgttggaa gatctcgccc ccggagcccc ggccacccta 14881 cgctggcccc tctaccgcca gccgccgccg cactttttgg tgggatacca gtacctggtg 14941 cggacttgca acgactacgt atttgactcg agggcttact cgcgtctcag gtacaccgag 15001 ctctcgcagc cgggtcacca gaccgttaac tggtccgtta tggccaactg cacttacacc 15061 atcaacacgg gcgcatacca ccgctttgtg gacatggatg acttccagtc taccctcacg 15121 caggtgcagc aggccatatt agccgagcgc gttgtcgccg acctagccct gcttcagccg 15181 atgaggggct tcggggtcac acgcatggga ggaagagggc gccacctacg gccaaactcc 15241 gccgccgccg cagcgataga tgcaagagat gcaggacaag aggaaggaga agaagaagtg 15301 ccggtagaaa ggctcatgca agactactac aaagacctgc gccgatgtca aaacgaagcc 15361 tggggcatgg ccgaccgcct gcgcattcag caggccggac ccaaggacat ggtgcttctg //
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