| 质粒名称: | PL-SIN-PGK-EGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21316 |
| 存储实验室: | James Ellis |
|
载体骨架基本信息 |
|
| 载体名称: | PL-SIN lentiviral vector |
| 载体出品公司: | Buzina et al., PLoS Genetics, 2008 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6531 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Enhanced Green Fluorescence Protein,EGFP |
| 物种来源: | -- |
| 插入大小: | 720 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | PGK |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PL-SIN-PGK-EGFP was a gift from James Ellis (Addgene plasmid # 21316 ; http://n2t.net/addgene:21316 ; RRID:Addgene_21316) |
| 参考文献部分: | Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS, Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J. Nat Methods. 2009 May . 6(5):370-6. 10.1038/nmeth.1325 PubMed 19404254 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PL-SIN-PGK-EGFP 6846 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Control Lentiviral vector with ubiquitous PGK promoter, expresses
EGFP.
ACCESSION .
VERSION .
KEYWORDS PL-SIN-PGK-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6846)
AUTHORS Hotta A, Cheung AY, Farra N, Vijayaragavan K, Seguin CA, Draper JS,
Pasceri P, Maksakova IA, Mager DL, Rossant J, Bhatia M, Ellis J
TITLE Isolation of human iPS cells using EOS lentiviral vectors to select
for pluripotency.
JOURNAL Nat Methods. 2009 May . 6(5):370-6.
PUBMED 19404254
REFERENCE 2 (bases 1 to 6846)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6846
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 364..386
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 378..395
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
LTR 785..1042
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1693..1810
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2768..3267
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2803..2826)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3200..3219
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 3281..3290
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3287..4006
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3332..3353)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3593..3612)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3940..3961
/label=EGFP-C
/note="EGFP, forward primer"
LTR 4133..4366
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(4403..4425)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4414..4430
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4438..4468)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4483..4504
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4621..4638)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4792..5380)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4872..4891)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6173..6192
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6411..6515)
/gene="bla"
/label=AmpR promoter
promoter complement(6552..6748)
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 6566..6701
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6620..6639)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 6819..6837
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gcggccgctg gaagggctaa
421 tttggtccca aaaaagacaa gagatccttg atctgtggat ctaccacaca caaggctact
481 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
541 ggtgcttcaa gttagtacca gttgaaccag agcaagtaga agaggccaaa taaggagaga
601 agaacagctt gttacaccct atgagccagc atgggatgga ggacccggag ggagaagtat
661 tagtgtggaa gtttgacagc ctcctagcat ttcgtcacat ggcccgagag ctgcatccgg
721 agtactacaa agactgctga catcgagctt tctacaaggg actttccgct ggggactttc
781 cagggaggtg tggcctgggc gggactgggg agtggcgagc cctcagatgc tacatataag
841 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
901 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgctcaaa
961 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
1021 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagtaaagc
1081 cagaggagat ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
1141 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
1201 ggtgcgagag cgtcggtatt aagcggggga gaattagata aatgggaaaa aattcggtta
1261 aggccagggg gaaagaaaca atataaacta aaacatatag tatgggcaag cagggagcta
1321 gaacgattcg cagttaatcc tggcctttta gagacatcag aaggctgtag acaaatactg
1381 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
1441 atagcagtcc tctattgtgt gcatcaaagg atagatgtaa aagacaccaa ggaagcctta
1501 gataagatag aggaagagca aaacaaaagt aagaaaaagg cacagcaagc agcagctgac
1561 acaggaaaca acagccaggt cagccaaaat taccctatag tgcagaacct ccaggggcaa
1621 atggtacatc aggccatatc acctagaact ttaaattaag acagcagtac aaatggcagt
1681 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
1741 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
1801 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggaaag gaccagcaaa
1861 gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca taaaagtagt
1921 gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa cagatggcag gtgatgattg
1981 tgtggcaagt agacaggatg aggattaaca catggaaaag attagtaaaa caccatagct
2041 ctagagcgat ccgatcttca gacctggagg aggcgatatg agggacaatt ggagaagtga
2101 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
2161 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
2221 cttgggagca gcaggaagca ctatgggctg cacgtcaatg acgctgacgg tacaggccag
2281 acaattattg tctgatatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
2341 acagcatctg ttgcaactca cagtctgggg catcaaacag ctccaggcaa gaatcctggc
2401 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
2461 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
2521 ttggaataac atgacctgga tggagtggga cagagaaatt aacaattaca caagcttggt
2581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
2641 accattatcg tttcagaccc acctcccaat cccgagggga cccgacaggc ccgaaggaat
2701 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctaa
2761 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
2821 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
2881 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
2941 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
3001 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
3061 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
3121 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
3181 aggtcctccc gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
3241 tcctcttcct catctccggg cctttcgacc tgcagcggtc gccaccatgg tgagcaaggg
3301 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
3361 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
3421 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
3481 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
3541 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
3601 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
3661 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
3721 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
3781 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
3841 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
3901 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
3961 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccaacctcg
4021 acgttattcc cttcgcgaag gcctatcgat ttcgaacccg gggtaccttt aagaccaatg
4081 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
4141 ctaattcact cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta
4201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
4261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
4321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagaat tctagcttgg
4381 cgtaatcatg gtcatagctg ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
4441 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
4501 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
4561 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
4621 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
4681 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
4741 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
4801 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
4861 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
4921 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
4981 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
5041 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
5101 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
5161 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
5221 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccactta ccttcggaaa
5281 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
5341 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
5401 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
5461 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
5521 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
5581 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
5641 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
5701 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
5761 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
5821 aagtagttcg ccagttaata gtttgcgcaa cgttgtgcca ttgctacagg catcgtggtg
5881 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
5941 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
6001 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
6061 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
6121 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
6181 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
6241 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
6301 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
6361 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
6421 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
6481 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
6541 gggactagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
6601 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
6661 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
6721 actatggttg ctgactaatt gagatgtagc ttgcatgccg acatggatta ttgactagtc
6781 cctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
6841 ttcgtc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pWPXLd
- 下一篇：pLVPT-tTR-KRAB
