| 质粒名称: | pCX-cMyc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 19772 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCX |
| 载体出品公司: | Jun-ichi Miyazaki |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4778 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | c-Myc |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1350 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | GCGAGCCGCAGCCATTGCCTTTTA |
| 3' 测序引物 | TTAGCCAGAAGTCAGATGCTC |
|
文献引用方法 |
|
| 材料和方法部分: | pCX-cMyc was a gift from Shinya Yamanaka (Addgene plasmid # 19772 ; http://n2t.net/addgene:19772 ; RRID:Addgene_19772) |
| 参考文献部分: | Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S. Science. 2008 Oct 9. ():. 10.1126/science.1164270 PubMed 18845712 |
买家导航
pCXcMyc载体基本信息
pCXcMyc载体图谱质粒图谱和多克隆位点信息
pCXcMyc载体简介
pCXcMyc载体序列
LOCUS pCX-cMyc 6131 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Non-integrating (episomal) expression of mouse c-Myc.
ACCESSION .
VERSION .
KEYWORDS pCX-cMyc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6131)
AUTHORS Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S
TITLE Generation of Mouse Induced Pluripotent Stem Cells Without Viral
Vectors.
JOURNAL Science. 2008 Oct 9. ():.
PUBMED 18845712
REFERENCE 2 (bases 1 to 6131)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6131
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..661
/label=chicken beta-actin promoter
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(3152..3171)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 3217..3272
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3271..3290)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3633..3649)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3633..3649)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3646..3668)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3657..3673
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3681..3711)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3726..3747
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3806..4001
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3852..3987
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3914..3933
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4007..4141
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4044..4063)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4098..4117
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4209..4226)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4380..4968)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4460..4479)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5139..5999)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5762..5781
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6000..6104)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
1741 ccatgcccct caacgtgaac ttcaccaaca ggaactatga cctcgactac gactccgtac
1801 agccctattt catctgcgac gaggaagaga atttctatca ccagcaacag cagagcgagc
1861 tgcagccgcc cgcgcccagt gaggatatct ggaagaaatt cgagctgctt cccaccccgc
1921 ccctgtcccc gagccgccgc tccgggctct gctctccatc ctatgttgcg gtcgctacgt
1981 ccttctcccc aagggaagac gatgacggcg gcggtggcaa cttctccacc gccgatcagc
2041 tggagatgat gaccgagtta cttggaggag acatggtgaa ccagagcttc atctgcgatc
2101 ctgacgacga gaccttcatc aagaacatca tcatccagga ctgtatgtgg agcggtttct
2161 cagccgctgc caagctggtc tcggagaagc tggcctccta ccaggctgcg cgcaaagaca
2221 gcaccagcct gagccccgcc cgcgggcaca gcgtctgctc cacctccagc ctgtacctgc
2281 aggacctcac cgccgccgcg tccgagtgca ttgacccctc agtggtcttt ccctacccgc
2341 tcaacgacag cagctcgccc aaatcctgta cctcgtccga ttccacggcc ttctctcctt
2401 cctcggactc gctgctgtcc tccgagtcct ccccacgggc cagccctgag cccctagtgc
2461 tgcatgagga gacaccgccc accaccagca gcgactctga agaagagcaa gaagatgagg
2521 aagaaattga tgtggtgtct gtggagaaga ggcaaacccc tgccaagagg tcggagtcgg
2581 gctcatctcc atcccgaggc cacagcaaac ctccgcacag cccactggtc ctcaagaggt
2641 gccacgtctc cactcaccag cacaactacg ccgcaccccc ctccacaagg aaggactatc
2701 cagctgccaa gagggccaag ttggacagtg gcagggtcct gaagcagatc agcaacaacc
2761 gcaagtgctc cagccccagg tcctcagaca cggaggaaaa cgacaagagg cggacacaca
2821 acgtcttgga acgtcagagg aggaacgagc tgaagcgcag cttttttgcc ctgcgtgacc
2881 agatccctga attggaaaac aacgaaaagg cccccaaggt agtgatcctc aaaaaagcca
2941 ccgcctacat cctgtccatt caagcagacg agcacaagct cacctctgaa aaggacttat
3001 tgaggaaacg acgagaacag ttgaaacaca aactcgaaca gcttcgaaac tctggtgcat
3061 aaaagggcga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
3121 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
3181 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
3241 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
3301 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
3361 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
3421 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
3481 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
3541 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
3601 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
3661 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
3721 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
3781 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
3841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
3901 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
3961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
4021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
4081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
4141 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4921 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4981 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5041 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5101 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5161 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5221 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5281 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5341 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5401 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5461 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5521 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5581 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5641 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5701 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
5761 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5821 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5881 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5941 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6001 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6061 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6121 gtgccacctg g
//
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