pGAD424

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pGAD424
质粒类型: 酵母单杂交系统
高拷贝/低拷贝: --
克隆方法: --
启动子: ADH1
载体大小: 6659 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pGAD424载体图谱

载体简介

载体序列

LOCUS       pGAD424	6659 bp 	DNA	SYN
DEFINITION  pGAD424
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="pGAD424"
                     /mol_type="other DNA"
     promoter        10..406
                     /label="yADH1_promoter"
     misc_feature    422..829
                     /label="Gal4_AD"
     CDS             422..877
                     /label="ORF frame 2"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    431..487
                     /label="SV40_NLS"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    452..472
                     /label="SV40 NLS"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    458..472
                     /label="NLS"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    491..829
                     /label="Gal4_AD"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    755..772
                     /label="Gal4_AD_primer"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     terminator      1104..1427
                     /label="yADH2_terminator"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    complement(1120..1143)
                     /label="pJG4_5_rev_primer"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     terminator      1241..1432
                     /label="yADH1_terminator"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     misc_feature    complement(1296..1320)
                     /label="pHybLex_rev_primer"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     gene            complement(1546..2640)
                     /label="LEU2 (variant)"
                     /gene="LEU2 (variant)"
                     /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS
                     NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN
                     AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*"
     CDS             complement(1546..2640)
                     /label="ORF frame 3"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     promoter        complement(3083..3101)
                     /label="M13_reverse_primer"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     misc_feature    complement(3100..3122)
                     /label="M13_pUC_rev_primer"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     promoter        complement(3136..3165)
                     /label="lac_promoter"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     gene            complement(4248..5108)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     CDS             complement(4248..5108)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5150..5178)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(5337..5359)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5495..6659
                     /label="2micron2_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
   61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
  121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
  181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
  241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
  301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
  361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa
  421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg
  481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt
  541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc
  601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag
  661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc
  721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata
  781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc
  841 ggggatccgt cgacctgcag agatctatga atcgtagata ctgaaaaacc ccgcaagttc
  901 acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg ttttgccttc
  961 ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct gatctataga
 1021 attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt cttcatgaaa
 1081 atatattacg agggcttatt cagaagcttt ggacttcttc gccagaggtt tggtcaagtc
 1141 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg
 1201 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat
 1261 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
 1321 ttaggtttta aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct
 1381 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcccgaaat
 1441 tcccctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat tatgaatttc
 1501 atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag caaggatttt
 1561 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc
 1621 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc
 1681 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg
 1741 gtcaacctta ttctttggca aatctggagc agaaccgtgg catggttcgt acaaaccaaa
 1801 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc
 1861 tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat
 1921 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg
 1981 ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt ttctccataa
 2041 tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg gtggctcatg
 2101 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg
 2161 ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa agtaaatacc
 2221 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg
 2281 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg
 2341 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactacctg taccccattt
 2401 aggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt ccagcgcctc
 2461 atctggaagt gggacacctg tagcatcgat agcagcacca ccaattaaat gattttcgaa
 2521 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc
 2581 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gggcagacat
 2641 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag
 2701 ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata
 2761 ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat
 2821 gaaaagccgg ttccggcctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg
 2881 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct
 2941 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga
 3001 gattcgaact cttgcatctt acgatacctg agtattccca cagttgggga tctcgactct
 3061 agctagagga tcaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
 3121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
 3181 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
 3241 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
 3301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
 3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
 3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
 3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
 3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
 3601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
 3661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
 3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
 3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
 3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
 3901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
 3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
 4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
 4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
 4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
 4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
 4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
 4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
 4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
 4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
 4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
 4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
 4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
 4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
 4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
 4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
 4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
 4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
 4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
 5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
 5101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
 5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
 5221 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
 5281 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
 5341 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
 5401 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
 5461 gcatcagagc agattgtact gagagtgcac cataacgcat ttaagcataa acacgcacta
 5521 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa
 5581 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg
 5641 ctttgaagtt cctattccga agttcctatt ctctagctag aaagtatagg aacttcagag
 5701 cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg
 5761 actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat
 5821 ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc
 5881 cttgaacttg catctaaact cgacctctac attttttatg tttatctcta gtattactct
 5941 ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat
 6001 gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc
 6061 tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca
 6121 tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct
 6181 agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac
 6241 caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc
 6301 attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg
 6361 ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag
 6421 cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat
 6481 tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa
 6541 aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
 6601 aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga tgcttcgtt

//

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