PX385

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: PX385 或 PX385 Streptococcus pasteurianus Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10586 bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: --
产品目录号: #68322
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX385载体图谱



载体简介

载体序列

LOCUS       PX385 Streptococcus pasteurianus Cas9	10586 bp 	DNA	SYN
DEFINITION  PX385 Streptococcus pasteurianus Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="PX385 Streptococcus pasteurianus Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5277
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    complement(5310..5327)
                     /label="Sp6_primer"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    5332..5356
                     /label="attB2"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     terminator      5368..5834
                     /label="hGH_PA_terminator"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     terminator      5840..5875
                     /label="T7_terminator"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     rep_origin      5992..6298
                     /label="f1_origin"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    complement(6412..6432)
                     /label="pBABE_3_primer"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    complement(6418..6633)
                     /label="SV40_enhancer"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     promoter        6430..6698
                     /label="SV40_promoter"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     rep_origin      6597..6674
                     /label="SV40_origin"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     misc_feature    6659..6678
                     /label="SV40pro_F_primer"
                     /translation="MYPYDVPDYASPKKKRKVEASMTNGKILGLDIGIASVGVGIIEA
                     KTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYGIVTDFR
                     NLNLNPYELRVKGLTEQLKNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDE
                     NRRLLKNKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILE
                     TQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILI
                     GKCNFYPDEYRASKASYTAQEYNFLNDLNNLKVSTETGKLSTEQKESLVEFAKNTATL
                     GPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLESINIDDLSREV
                     IDKLADILTLNTEREGIEDAIKRNLPNQFTEEQISEIIKVRKSQSTAFNKGWHSFSAK
                     LMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVR
                     QTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLY
                     NSSDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPISIQELVHNSNNFEIDHILPL
                     SLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKR
                     DYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKVSVVRGQFT
                     SQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGKNQVVDKQTGE
                     ILSVSDDEYKELVFQPPYQGFVNTISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRK
                     AKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDSLTWENVIEVIL
                     RDYPTTKKSEDGKNDVKCNPFEEYRRENGLICKYSKKGKGTPIKSLKYYDKKLGNCID
                     ITPEESRNKVILQSINPWRADVYFNPETLKYELMGLKYSDLSFEKGTGNYHISQEKYD
                     AIKEKEGIGKKSEFKFTLYRNDLILIKDIASGEQEIYRFLSRTMPNVNHYVELKPYDK
                     EKFDNVQELVEALGEADKVGRCIKGLNKPNISIYKVRTDVLGNKYFVKKKGDKPKLDF
                     KNNKK*"
     CDS             6813..7607
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6816..7604
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(7122..7658)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7784..7903
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7872..7891
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      8094..8129
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8152..8169)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8159..8236
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8266..8883)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8863..8896
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9664..10524)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9664..10524)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgact aacggcaaga ttctggggct ggacattggc
 1921 atcgcaagcg tgggggtggg gattattgag gcaaaaactg gaaaggtggt gcatgccaat
 1981 tcccggctgt tctctgccgc taacgctgag aacaatgcag aacggagagg gtttagggga
 2041 tctaggcgcc tgaatcgacg gaagaaacac cgcgtgaagc gagtccggga tctgttcgag
 2101 aaatacggaa tcgtcaccga ctttcgcaac ctgaatctga acccttatga gctgcgagtg
 2161 aagggcctga ccgaacagct gaaaaacgag gaactgttcg cagccctgag aacaatctct
 2221 aagagaaggg ggattagtta cctggacgat gccgaggacg atagtaccgg atcaacagac
 2281 tatgctaaga gcatcgatga gaatcgccga ctgctgaaaa acaagacacc aggccagatt
 2341 cagctggaga ggctggaaaa gtacggccag ctgcgcggga atttcaccgt ctatgacgag
 2401 aacggggaag cccatcgcct gatcaatgtg tttagtacat cagattacga gaaagaagca
 2461 cggaagatcc tggagacaca ggccgactac aacaagaaaa tcacagctga gttcattgac
 2521 gattatgtgg aaatcctgac ccagaaacga aagtactatc acggccccgg gaacgaaaag
 2581 agccggactg actacggacg gttccggacc gatgggacca cactggagaa tattttcgga
 2641 atcctgattg gcaagtgcaa cttttaccct gatgaatatc gagcaagcaa ggccagctac
 2701 accgcacagg agtataattt cctgaacgac ctgaacaatc tgaaggtgag caccgaaaca
 2761 gggaagctgt caacagagca gaaagaaagc ctggtggagt ttgccaagaa tactgctacc
 2821 ctgggacccg ctaaactgct gaaggagatc gcaaaaattc tggactgtaa ggtggatgag
 2881 atcaaaggat acagagagga cgataaaggc aagccagatc tgcatacctt cgagccctat
 2941 aggaaactga agtttaatct ggaaagcatc aacattgacg atctgtcccg cgaagtgatc
 3001 gacaagctgg ctgatattct gactctgaac accgagagag aaggaatcga ggacgcaatt
 3061 aagaggaatc tgccaaacca gttcacagag gaacagatca gcgagatcat caaggtgcgg
 3121 aagagccagt ccactgcttt caataagggc tggcactctt ttagtgcaaa actgatgaac
 3181 gagctgatcc ccgaactgta cgccacctcc gacgagcaga tgacaattct gactcggctg
 3241 gaaaaattca aggtcaataa gaaaagctcc aaaaacacaa agactatcga cgagaaggaa
 3301 gtcactgatg agatctacaa tcctgtggtc gccaagagcg tgagacagac catcaaaatc
 3361 attaacgctg cagtcaagaa atatggcgac ttcgataaga tcgtgattga aatgccacgg
 3421 gataaaaatg ctgacgatga gaagaagttc atcgacaaga gaaataagga gaacaagaag
 3481 gaaaaggacg atgccctgaa aagggccgct tacctgtata attctagtga caagctgccc
 3541 gatgaggtgt tccacggcaa caagcagctg gaaaccaaaa tccgactgtg gtatcagcag
 3601 ggggagcggt gcctgtatag tggaaagccc atctcaattc aggagctggt gcataactct
 3661 aacaatttcg aaatcgatca cattctgcct ctgtcactga gctttgacga tagtctggcc
 3721 aataaggtgc tggtctacgc ttggacaaac caggagaaag gccagaaaac cccttatcag
 3781 gtcatcgact ccatggatgc agcctggtct ttcagggaga tgaaggacta cgtgctgaaa
 3841 cagaagggac tgggcaagaa aaagcgcgac tatctgctga ctaccgagaa catcgataag
 3901 attgaagtga agaagaagtt catcgagagg aatctggtgg atactcgcta cgcatctcga
 3961 gtggtcctga actctctgca gagtgccctg agagagctgg ggaaagacac taaggtgtct
 4021 gtggtcaggg gacagttcac cagtcagctg cggagaaaat ggaagatcga taagagccgc
 4081 gagacatacc accatcacgc agtggacgcc ctgatcattg ctgcatcaag ccagctgaaa
 4141 ctgtgggaga agcaggacaa tcccatgttt gtggattatg gcaagaacca ggtggtcgac
 4201 aaacagactg gggagatcct gtccgtgtct gacgatgagt acaaggaact ggtgttccag
 4261 cccccttatc agggctttgt gaataccatc tcctctaaag ggttcgagga cgaaattctg
 4321 tttagctacc aggtggattc caaatataac cggaaggtca gtgacgcaac catctactca
 4381 acaagaaaag ccaagattgg caaggataag aaagaggaaa cctacgtgct gggaaaaatc
 4441 aaggacatct actcccagaa tggcttcgat accttcatca agaagtacaa caaagataag
 4501 actcagttcc tgatgtatca gaaggactct ctgacatggg agaacgtgat cgaagtcatt
 4561 ctgagggact acccaacaac taagaaaagc gaggacggca aaaatgatgt gaagtgcaac
 4621 ccctttgagg aatacaggcg cgagaatggg ctgatctgta agtattccaa gaaagggaaa
 4681 ggaactccca tcaagagcct gaagtactat gacaagaaac tggggaactg catcgatatt
 4741 accccagagg aatcacgcaa taaggtcatc ctgcagagca ttaacccttg gcgagccgac
 4801 gtgtacttca atccagagac actgaagtac gaactgatgg gcctgaaata ttcagatctg
 4861 agctttgaaa agggcactgg gaactaccat atcagccagg agaaatatga cgctatcaaa
 4921 gagaaggaag gaattggcaa gaaatccgag ttcaagttta cactgtaccg caacgacctg
 4981 atcctgatca aggatatcgc cagtggcgag caggaaatct acagattcct gtcaagaact
 5041 atgcccaatg tgaaccacta cgtcgagctg aagccttacg acaaggaaaa gttcgataac
 5101 gtgcaggagc tggtcgaagc actgggagag gcagataaag tgggacgatg tatcaaagga
 5161 ctgaataagc caaacatcag catctacaag gtgagaaccg acgtcctggg aaacaaatat
 5221 ttcgtgaaga aaaagggcga caaacccaag ctggatttta agaacaacaa gaagtaagaa
 5281 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt
 5341 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
 5401 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
 5461 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
 5521 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
 5581 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
 5641 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
 5701 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
 5761 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
 5821 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt
 5881 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
 5941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
 6001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
 6061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
 6121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
 6181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
 6241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
 6301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
 6361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
 6421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
 6481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
 6541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
 6601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
 6661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
 6721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
 6781 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
 6841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
 6901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
 6961 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
 7021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
 7081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
 7141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
 7201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
 7261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
 7321 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
 7381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
 7441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
 7501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
 7561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
 7621 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
 7681 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
 7741 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
 7801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
 7861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag
 7921 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
 7981 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
 8041 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc
 8101 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct
 8161 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac
 8221 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc
 8281 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata
 8341 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa
 8401 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg
 8461 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact
 8521 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa
 8581 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt
 8641 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg
 8701 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc
 8761 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc
 8821 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata
 8881 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt
 8941 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
 9001 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
 9061 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 9241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 9481 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
 9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 9661 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
 9721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
 9781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
 9841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
 9901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
 9961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
10021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
10081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
10141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
10201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
10441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
10501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
10561 gggttattgt ctcatgtccg cgcgtt
//

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