| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBMTB-3 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 4831 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBMTB-3载体质粒基本信息
pBMTB-3质粒图谱载体图谱和pBMTB-3载体序列质粒序列多克隆位点信息
pBMTB-3质粒载体简介
pBMTB-3质粒序列载体序列
LOCUS DQ058726 4831 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBMTB-3, complete sequence.
ACCESSION DQ058726
VERSION DQ058726.1 GI:69048213
TITLE Broad host range vectors for stable genomic library construction
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..4831
/organism="Broad host range vector pBMTB-3"
/mol_type="other DNA"
/db_xref="taxon:334958"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene complement(104..1123)
/gene="mob"
/note="mob gene from pBBR1MCS, GenBank Accession Number
U02374"
CDS complement(104..1123)
/gene="mob"
/note="required for mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AAY99705.1"
/db_xref="GI:69048214"
/translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
KPLLS"
gene 2117..2779
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 2117..2779
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99706.1"
/db_xref="GI:69048215"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 2867..3535
/gene="cat"
CDS 2876..3535
/gene="cat"
/codon_start=1
/transl_table=11
/product="chloramphenicol acetyl transferase"
/protein_id="AAY99707.1"
/db_xref="GI:69048216"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
regulatory 3554..3585
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(3613..4542)
/gene="araC"
CDS complement(3613..4542)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99708.1"
/db_xref="GI:69048217"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 4550..4817
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
2881 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
2941 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
3001 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
3061 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
3121 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
3181 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
3241 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
3301 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
3361 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
3421 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
3481 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
3541 tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
3601 caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt cacaaccggc
3661 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
3721 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
3781 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
3841 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
3901 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
3961 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
4021 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
4081 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
4141 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
4201 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
4261 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
4321 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
4381 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
4441 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
4501 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
4561 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
4621 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
4681 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
4741 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
4801 atacccgttt ttttgggcta gagaattcgt c
//
