LOCUS pCMV-MmPeg10rc4 7075 bp ds-DNA circular SYN 14-SEP-2021
DEFINITION Expresses recoded mouse Peg10 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-MmPeg10rc4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7075)
AUTHORS Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
RK, Koonin EV, Zhang F
TITLE Mammalian retrovirus-like protein PEG10 packages its own mRNA and
can be pseudotyped for mRNA delivery.
JOURNAL Science. 2021 Aug 20;373(6557):882-889. doi:
10.1126/science.abg6155.
PUBMED 34413232
REFERENCE 2 (bases 1 to 7075)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 14, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7075
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 718..1193
/label=-beta-globin intron
/note="internally truncated intron from human beta-globin"
CDS 4146..4172
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
polyA_signal 4257..4651
/label=-beta-globin poly(A)
/note="human beta-globin polyadenylation signal"
primer_bind complement(4990..5007)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5161..5749)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5241..5260)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5920..6780)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6543..6562
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6781..6885)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6955..6974)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg
721 agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat
781 aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt
841 aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact
901 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca
961 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa
1021 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac
1081 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc
1141 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg
1201 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag atctgaattc
1261 cccaagctgg ctagcgttta aaaccgccac atggctgctg caggtggttc ctccaactgc
1321 ccgccccctc cccctccccc tcctcccaac aacaacaaca acaacaacac cccaaagagc
1381 ccaggcgtgc ctgacgccga agatgatgat gaacgcagac acgatgagct ccctgaagac
1441 atcaacaact ttgacgaaga catgaacagg cagtttgaga atatgaacct gctggatcag
1501 gtggagttgc ttgcacagag ctacagtctg ctggatcatt tagatgactt tgatgatgat
1561 gatgaagacg atgactttga tccagaacct gaccaggatg agctccctga gtacagtgac
1621 gatgatgacc tggagcttca gggtgctgca gcagccccta tcccaaactt tttctccgat
1681 gatgactgcc ttgaagacct tcctgagaag ttcgatggca accctgacat gctgggtcct
1741 ttcatgtatc agtgccagct cttcatggaa aagagcacca gagatttctc agttgaccgc
1801 atccgtgtgt gcttcgtgac aagcatgctg atcggccgtg ccgcccgctg ggctactgcc
1861 aagctgcaaa gatgtactta cctgatgcac aactacactg cctttatgat ggagctgaag
1921 catgtctttg aagaccctca gagacgtgaa gctgccaaac gcaagatcag acgtctgcgc
1981 cagggccctg ggcctgttgt ggactactcc aatgcattcc agatgattgc ccaggacctg
2041 gattggactg agcctgccct gatggatcag ttccaggaag gtctcaaccc agacattcgc
2101 gcagagctgt ctcgccagga ggcccccaag accctggctg ctctgattac tgcctgtatt
2161 cacatcgaga gaaggctggc tcgtgacgct gctgcaaagc ccgatccttc acccagagcc
2221 ttggtgatgc ctccaaacag ccagaccgat cccaccgagc ctgtgggagg tgcccgcatg
2281 cgcctgtcca aggaagaaaa ggagagacgc cgcaaaatga atttgtgtct ctactgtggc
2341 aatggaggcc atttcgccga cacgtgtcca gcgaaagcct ccaagaattc gccgccggga
2401 aactccccgg ccccgctgta gggggacctt cagcgacagg gccagaacga ataaggtccc
2461 caccctccga ggcttcgact cagcacctgc aagtgatgct ccagattcat atgccgggca
2521 gacccaccct gtttgtccga gctatgattg attctggtgc atctggcaac ttcattgatc
2581 aagactttgt catacaaaat gcaattcctc tcagaatcaa agactggcca gtgatggtgg
2641 aagctattga tgggcatcca attgcctcgg gcccaatcat tttggaaacc caccacctga
2701 tagttgatct gggagaccac cgtgagatac tgtcatttga tgtgactcag tctccattct
2761 ttcctattgt cctaggaatt cgttggctct ctactcacga cccacacatc acctggagta
2821 ctcgctccat agtcttcaac tctgattact gtcggttgag atgccgaatg ttcgctcaga
2881 tcccaagcaa cctgctcttt accgttcccc aaccaaatct ccacccctat ctcctgcatc
2941 atgtacaccc ccatgtacac cctcacatgc atcaacacct tcatcaacac ctgcatcagt
3001 ttcttcatcc tgatccacat cagtatccac acccagaccc ccattaccat catcaccagc
3061 aggctgatat gcagcatcag ctgcaacagt atttgtatca gtacttgtac taccatctgt
3121 accctgtcat gcatcatcat cttcctcctg accaacatga acatctgcac gaatatcttc
3181 accaatacct ccatcagtac cttcaccaat tcctccacca ccatcttcac cctgacttgc
3241 accaatattt gtaccagtat cttcacaacc acatgaatcc agatccacat caccatcccc
3301 atccagatcc ccctcaggat ccacatcacc ctccacatca ggatccccat caggatcctc
3361 cacatcagga tccacatcag gatgcacatc aggatcccca tatggatcca cacctgcatc
3421 agcaccagca tccgcagccg cagccgcatc cacaacagca tcctaaccat cctcagcagc
3481 caccattctt ctaccacatg gctggattca gaatttacca ccctgtaagg tattactata
3541 ttcagaatgt gtatacacct gttgatgagc atgtctatcc gggtcaccgg gtggttgacc
3601 ctaacattga gatgattcct ggagcgcaca gcctgcccag tggacatttg tactcaatgt
3661 ctgagtctga aatgaatgct ctgcgaaatt tcgtggacag gaatgttaaa gatgggctca
3721 tgactcccac tgtggcgccc aatggagccc aagtcctgca agtgaaaaga gggtggaaac
3781 tccaagtcac ttacaattgc cgagctccac agagtggcac catccaaaat cagtacctac
3841 gcatgtctct tccaaatatg ggagaccctg cacacctggc aagctatggt gaatttgtcc
3901 aagttcctgg ctacccatat ccagcctatg tttactatac aagcccgcat atgatgactg
3961 cgtggtaccc agtaggacga gatgtacatg gacgaataat cgttgtgcct gttgtaatca
4021 cctggtctca aaatacgaac cgccagcctc cggtgcccca gtatcctcct ccgcagccac
4081 ctccaccacc accaccacct ccaccgccac caccacctcc accagcatca tcctgcagtg
4141 ctgcgtatcc gtatgatgtt ccggattatg catgagaatt caccccacca gtgcaggctg
4201 cctatcagaa agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc
4261 gctttcttgc tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa
4321 ctgggggata ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt
4381 ttcattgcaa tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga
4441 ggtcagtgca tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac
4501 tatatcttaa actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca
4561 gcccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt
4621 ggaggttaaa gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg
4681 aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt
4741 ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat
4801 ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact
4861 tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc
4921 ccccactcac agtgacccgg aatccctcga catggcagtc tagcactagt gcggccgcag
4981 atctgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
5041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
5101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
5161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
5221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
5281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
5341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
5401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
5461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
5521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
5581 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
5641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
5701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
5761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
5821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
5881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
5941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
6001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
6061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
6121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
6181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
6241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
6301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
6361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
6421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
6481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
6541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
6601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
6661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
6721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
6781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
6841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
6901 agtgccacct gacgtggatc ccctgagggg gcccccatgg gctagaggat ccggcctcgg
6961 cctctgcata aataaaaaaa attagtcagc catgagcttg gcccattgca tacgttgtat
7021 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgtt
//
