| 质粒名称: | pCXLE-hUL |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27080 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCXLE |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 10180 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
| 宿生菌： | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | L-MYC |
| 备用名称： | LIN28 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1871bp |
| Entrez基因： |
LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A) MYCL (a.k.a. L-Myc, LMYC, MYCL1, bHLHe38) |
| 克隆方法: | 限制性酶 |
| 5' 克隆位点: | Ecori（未销毁） |
| 3' 克隆位点: | Ecori（未销毁） |
| 5' 测序引物 | pCAG-F |
| 3' 测序引物 | WPRE-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCXLE-hUL was a gift from Shinya Yamanaka (Addgene plasmid # 27080 ; http://n2t.net/addgene:27080 ; RRID:Addgene_27080) |
| 参考文献部分: | A more efficient method to generate integration-free human iPS cells. Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. Nat Methods. 2011 Apr 3. ():.10.1038/nmeth.1591 PubMed 21460823 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCXLE-hUL 12051 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Integration-free (episomal) expression of human L-MYC and LIN28.
ACCESSION .
VERSION .
KEYWORDS pCXLE-hUL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12051)
AUTHORS Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong
H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
M, Takahashi J, Saji H, Yamanaka S
TITLE A more efficient method to generate integration-free human iPS
cells.
JOURNAL Nat Methods. 2011 Apr 3. ():.
PUBMED 21460823
REFERENCE 2 (bases 1 to 12051)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12051
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 385..661
/label=chicken beta-actin promoter
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 2859..2924
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
misc_feature 3629..4217
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3682..3702)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4100..4111)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4307..4326)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 4372..4427
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(4426..4445)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(4788..4804)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4788..4804)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4801..4823)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4812..4828
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4836..4866)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4881..4902
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind complement(4991..5024)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 5485..7410
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
protein_bind complement(7578..7611)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin 7798..9587
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(10129..10146)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10300..10888)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10380..10399)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11059..11919)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11682..11701
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11920..12024)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
541 ggggcgggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
1741 ccatggacta cgactcgtac cagcactatt tctacgacta tgactgcggg gaggatttct
1801 accgctccac ggcgcccagc gaggacatct ggaagaaatt cgagctggtg ccatcgcccc
1861 ccacgtcgcc gccctggggc ttgggtcccg gcgcagggga cccggccccc gggattggtc
1921 ccccggagcc gtggcccgga gggtgcaccg gagacgaagc ggaatcccgg ggccactcga
1981 aaggctgggg caggaactac gcctccatca tacgccgtga ctgcatgtgg agcggcttct
2041 cggcccggga acggctggag agagctgtga gcgaccggct cgctcctggc gcgccccggg
2101 ggaacccgcc caaggcgtcc gccgccccgg actgcactcc cagcctcgaa gccggcaacc
2161 cggcgcccgc cgccccctgt ccgctgggcg aacccaagac ccaggcctgc tccgggtccg
2221 agagcccaag cgactcggag aatgaagaaa ttgatgttgt gacagtagag aagaggcagt
2281 ctctgggtat tcggaagccg gtcaccatca cggtgcgagc agaccccctg gatccctgca
2341 tgaagcattt ccacatctcc atccatcagc aacagcacaa ctatgctgcc cgttttcctc
2401 cagaaagctg ctcccaagaa gaggcttcag agaggggtcc ccaagaagag gttctggaga
2461 gagatgctgc aggggaaaag gaagatgagg aggatgaaga gattgtgagt cccccacctg
2521 tagaaagtga ggctgcccag tcctgccacc ccaaacctgt cagttctgat actgaggatg
2581 tgaccaagag gaagaatcac aacttcctgg agcgcaagag gcggaatgac ctgcgttcgc
2641 gattcttggc gctgagggac caggtgccca ccctggccag ctgctccaag gcccccaaag
2701 tagtgatcct aagcaaggcc ttggaatact tgcaagccct ggtgggggct gagaagagga
2761 tggctacaga gaaaagacag ctccgatgcc ggcagcagca gttgcagaaa agaattgcat
2821 acctcactgg ctacggagat ctcaaaattg tcgctcctgt caaacaaact cttaactttg
2881 atttactcaa actggctggg gatgtagaaa gcaatccagg tccactcatg aacaattcgc
2941 ccttcaccat gggctccgtg tccaaccagc agtttgcagg tggctgcgcc aaggcggcag
3001 aagaggcgcc cgaggaggcg ccggaggacg cggcccgggc ggcggacgag cctcagctgc
3061 tgcacggtgc gggcatctgt aagtggttca acgtgcgcat ggggttcggc ttcctgtcca
3121 tgaccgcccg cgccggggtc gcgctcgacc ccccagtgga tgtctttgtg caccagagta
3181 agctgcacat ggaagggttc cggagcttga aggagggtga ggcagtggag ttcaccttta
3241 agaagtcagc caagggtctg gaatccatcc gtgtcaccgg acctggtgga gtattctgta
3301 ttgggagtga gaggcggcca aaaggaaaga gcatgcagaa gcgcagatca aaaggagaca
3361 ggtgctacaa ctgtggaggt ctagatcatc atgccaagga atgcaagctg ccaccccagc
3421 ccaagaagtg ccacttctgc cagagcatca gccatatggt agcctcatgt ccgctgaagg
3481 cccagcaggg ccctagtgca cagggaaagc caacctactt tcgagaggaa gaagaagaaa
3541 tccacagccc taccctgctc ccggaggcac agaattgaaa gggcgaattg cctgcaggaa
3601 ttcaagcttc ggggactagt catatgataa tcaacctctg gattacaaaa tttgtgaaag
3661 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
3721 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
3781 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
3841 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
3901 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
3961 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
4021 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
4081 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
4141 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
4201 ttgggccgcc tccccgcatc ggtaaattca ctcctcaggt gcaggctgcc tatcagaagg
4261 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
4321 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
4381 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
4441 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
4501 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
4561 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
4621 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
4681 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
4741 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
4801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
4861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
4921 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct caattccgat catattcaat
4981 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
5041 tttacgtcca gccaagctta ggatcaattc tcatgtttga cagcttatca tcgataagct
5101 gatcctcaca ggccgcaccc agcttttctt ccgttgcccc agtagcatct ctgtctggtg
5161 accttgaaga ggaagaggag gggtcccgag aatccccatc cctaccgtcc agcaaaaagg
5221 gggacgagga atttgaggcc tggcttgagg ctcaggacgc aaatcttgag gatgttcagc
5281 gggagttttc cgggctgcga gtaattggtg atgaggacga ggatggttcg gaggatgggg
5341 aattttcaga cctggatctg tctgacagcg accatgaagg ggatgagggt gggggggctg
5401 ttggaggggg caggagtctg cactccctgt attcactgag cgtcgtctaa taaagatgtc
5461 tattgatctc ttttagtgtg aatcatgtct gacgaggggc caggtacagg acctggaaat
5521 ggcctaggag agaagggaga cacatctgga ccagaaggct ccggcggcag tggacctcaa
5581 agaagagggg gtgataacca tggacgagga cggggaagag gacgaggacg aggaggcgga
5641 agaccaggag ccccgggcgg ctcaggatca gggccaagac atagagatgg tgtccggaga
5701 ccccaaaaac gtccaagttg cattggctgc aaagggaccc acggtggaac aggagcagga
5761 gcaggagcgg gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggagcagga
5821 ggaggggcag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
5881 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga
5941 ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga
6001 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
6061 gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagg ggcaggaggg
6121 gcaggagcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
6181 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggaggg
6241 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
6301 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggggca
6361 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
6421 gcaggaggag gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga
6481 ggcagtggag gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc
6541 cgccggggta gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga
6601 ggtcgtggac gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg
6661 tctccaccgc gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc
6721 gattattttg aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga
6781 gcgatagagc agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt
6841 cagggtgatg gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga
6901 ggttccaacc cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt
6961 cacgtagaaa ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt
7021 agtaagacct ccctttacaa cctaaggcga ggaactgccc ttgctattcc acaatgtcgt
7081 cttacaccat tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc
7141 ccgctaaggg agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct
7201 gaggttttga aggatgcgat taaggacctt gttatgacaa agcccgctcc tacctgcaat
7261 atcagggtga ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct
7321 atggtggaag gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat
7381 ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt
7441 attaaaagcc gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc
7501 tgttggtgta tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa
7561 ttgccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta ttaggtccct
7621 cgaagaggtt cactagcgga tctcaattgg gcatacccat gttgtcacgt cactcagctc
7681 cgcgctcaac accttctcgc gttggaaaac attagcgaca tttacctggt gagcaatcag
7741 acatgcgacg gctttagcct ggcctcctta aattcaccta agaatgggag caaccagcag
7801 gaaaaggaca agcagcgaaa attcacgccc ccttgggagg tggcggcata tgcaaaggat
7861 agcactccca ctctactact gggtatcata tgctgactgt atatgcatga ggatagcata
7921 tgctacccgg atacagatta ggatagcata tactacccag atatagatta ggatagcata
7981 tgctacccag atatagatta ggatagccta tgctacccag atataaatta ggatagcata
8041 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
8101 tgctacccag atatagatta ggatagcata tgctacccag atatagatta ggatagcata
8161 tgctatccag atatttgggt agtatatgct acccagatat aaattaggat agcatatact
8221 accctaatct ctattaggat agcatatgct acccggatac agattaggat agcatatact
8281 acccagatat agattaggat agcatatgct acccagatat agattaggat agcctatgct
8341 acccagatat aaattaggat agcatatact acccagatat agattaggat agcatatgct
8401 acccagatat agattaggat agcctatgct acccagatat agattaggat agcatatgct
8461 atccagatat ttgggtagta tatgctaccc atggcaacat tagcccaccg tgctctcagc
8521 gacctcgtga atatgaggac caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta
8581 atttgtcctc cagatcgcag caatcgcgcc cctatcttgg cccgcccacc tacttatgca
8641 ggtattcccc ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg cagtggttag
8701 cggggttaca atcagccaag ttattacacc cttattttac agtccaaaac cgcagggcgg
8761 cgtgtggggg ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt ggccacttgt
8821 ctttgtttat gggccccatt ggcgtggagc cccgtttaat tttcgggggt gttagagaca
8881 accagtggag tccgctgctg tcggcgtcca ctctctttcc ccttgttaca aatagagtgt
8941 aacaacatgg ttcacctgtc ttggtccctg cctgggacac atcttaataa ccccagtatc
9001 atattgcact aggattatgt gttgcccata gccataaatt cgtgtgagat ggacatccag
9061 tctttacggc ttgtccccac cccatggatt tctattgtta aagatattca gaatgtttca
9121 ttcctacact agtatttatt gcccaagggg tttgtgaggg ttatattggt gtcatagcac
9181 aatgccacca ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt
9241 tttcgagcac ctcacataca ccttactgtt cacaactcag cagttattct attagctaaa
9301 cgaaggagaa tgaagaagca ggcgaagatt caggagagtt cactgcccgc tccttgatct
9361 tcagccactg cccttgtgac taaaatggtt cactaccctc gtggaatcct gaccccatgt
9421 aaataaaacc gtgacagctc atggggtggg agatatcgct gttccttagg acccttttac
9481 taaccctaat tcgatagcat atgcttcccg ttgggtaaca tatgctattg aattagggtt
9541 agtctggata gtatatacta ctacccggga agcatatgct acccgtttag ggttaacaag
9601 ggggccttat aaacactatt gctaatgccc tcttgagggt ccgcttatcg gtagctacac
9661 aggcccctct gattgacgtt ggtgtagcct cccgtagtct tcctgggccc ctgggaggta
9721 catgtccccc agcattggtg taagagcttc agccaagagt tacacataaa ggcaatgttg
9781 tgttgcagtc cacagactgc aaagtctgct ccaggatgaa agccactcag tgttggcaaa
9841 tgtgcacatc catttataag gatgtcaact acagtcagag aacccctttg tgtttggtcc
9901 ccccccgtgt cacatgtgga acagggccca gttggcaagt tgtaccaacc aactgaaggg
9961 attacatgca ctgccccgcg aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc
10021 ggggcggggc atcgatcctc tagagtcgac gctagcggat ccgctgcatt aatgaatcgg
10081 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
10141 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
10201 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
10261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
10321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
10381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
10441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
10501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
10561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
10621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
10681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
10741 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
10801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
10861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
10921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
10981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
11041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
11101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
11161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
11221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
11281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
11341 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
11401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
11461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
11521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
11581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
11641 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
11701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
11761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
11821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
11881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
11941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
12001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg g
//
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