| 质粒名称: | pAAVS1P-iCAG.copGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 66577 |
| 存储实验室: | Jizhong Zou |
|
载体骨架基本信息 |
|
| 载体名称: | pAAVS1P-iRMCE |
| 空载体大小: | 10001 bp |
| 完整质粒大小: | 10760 bp |
| 载体修饰: | 在CAG启动子下克隆copGFP |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | copGFP |
| 物种来源: | Synthetic |
| 插入大小: | 759 bp |
| 启动子: | CAG |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsrGI(未破坏) |
| 3’克隆位点: | MluI (未破坏) |
| 5’测序引物 | gggcggggttcggcttctgg |
|
文献引用方法 |
|
| 材料和方法部分: | pAAVS1P-iCAG.copGFP was a gift from Jizhong Zou (Addgene plasmid # 66577 ; http://n2t.net/addgene:66577 ; RRID:Addgene_66577) |
| 参考文献部分: | Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J. PLoS One. 2015 Jan 14;10(1):e0116032. doi: 10.1371/journal.pone.0116032. eCollection 2015. PONE-D-14-41442 [pii] PubMed 25587899 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAVS1P-iCAG.copGFP 10760 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP,
compatible with pZT-AAVS1 TALENs.
ACCESSION .
VERSION .
KEYWORDS pAAVS1P-iCAG.copGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10760)
AUTHORS Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
TITLE Transcription activator-like effector nuclease (TALEN)-mediated
CLYBL targeting enables enhanced transgene expression and one-step
generation of dual reporter human induced pluripotent stem cell
(iPSC) and neural stem cell (NSC) lines.
JOURNAL PLoS One. 2015 Jan 14;10(1):e0116032. doi:
10.1371/journal.pone.0116032. eCollection 2015.
PUBMED 25587899
REFERENCE 2 (bases 1 to 10760)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10760
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(103..122)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 114..923
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(181..200)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 1101..1689
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1590..1609
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1843..1860
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 1977..1998
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2013..2043
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2051..2067
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2056..2078
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2075..2091
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2075..2091
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
misc_feature 2112..2915
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
protein_bind 2924..2957
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 2958..2983
/label=SA
/note="splice acceptor site"
CDS 3007..3060
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3064..3663
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3064..3083)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3560..3580
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 3664..3888
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 3889..3922
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind 5166..5199
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
enhancer 5270..5573
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5575..5852
/label=chicken beta-actin promoter
intron 5854..6871
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 6795..6817
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 6879..6898
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 6959..7621
/codon_start=1
/product="green fluorescent protein 2 from Pontellina
plumata, also known as ppluGFP2 (Shagin et al., 2004)"
/label=CopGFP
/translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
primer_bind complement(7783..7802)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 7848..7903
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(7902..7921)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
protein_bind complement(8236..8269)
/label=loxP511
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GTATACAT). loxP511 sites are compatible with each
other, but incompatible with wild-type loxP sites."
misc_feature 9521..10357
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(10366..10383)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(10366..10382)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10375..10397)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(10591..10610)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10710..10732
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
1981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
2041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
2101 gccgcggccg ctgctttctc tgaccagcat tctctcccct gggcctgtgc cgctttctgt
2161 ctgcagcttg tggcctgggt cacctctacg gctggcccag atccttccct gccgcctcct
2221 tcaggttccg tcttcctcca ctccctcttc cccttgctct ctgctgtgtt gctgcccaag
2281 gatgctcttt ccggagcact tccttctcgg cgctgcacca cgtgatgtcc tctgagcgga
2341 tcctccccgt gtctgggtcc tctccgggca tctctcctcc ctcacccaac cccatgccgt
2401 cttcactcgc tgggttccct tttccttctc cttctggggc ctgtgccatc tctcgtttct
2461 taggatggcc ttctccgacg gatgtctccc ttgcgtcccg cctccccttc ttgtaggcct
2521 gcatcatcac cgtttttctg gacaacccca aagtaccccg tctccctggc tttagccacc
2581 tctccatcct cttgctttct ttgcctggac accccgttct cctgtggatt cgggtcacct
2641 ctcactcctt tcatttgggc agctccccta ccccccttac ctctctagtc tgtgctagct
2701 cttccagccc cctgtcatgg catcttccag gggtccgaga gctcagctag tcttcttcct
2761 ccaacccggg cccctatgtc cacttcagga cagcatgttt gctgcctcca gggatcctgt
2821 gtccccgagc tgggaccacc ttatattccc agggccggtt aatgtggctc tggttctggg
2881 tacttttatc tgtcccctcc accccacagt ggggcgcggc cgcataactt cgtatagcat
2941 acattatacg aagttatctg acctcttctc ttcctcccac agggcctcga gagatctggc
3001 agcggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
3061 aggatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
3121 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
3181 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
3241 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
3301 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
3361 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
3421 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
3481 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
3541 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
3601 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
3661 tgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
3721 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
3781 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
3841 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggat aacttcgtat
3901 agcatacatt atacgaagtt atgagctcac ggggacagcc cccccccaaa gcccccaggg
3961 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
4021 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
4081 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
4141 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
4201 tagaacggcc tgggttgcaa aggagcacag tgctcatcca gatccaaccc cctgctatgt
4261 gcagggtcat caaccagcag cccaggctgc ccagagccac atccagcctg gccttgaatg
4321 cctgcaggga tggggcatcc acagcctcct tgggcaacct gttcagtgcg tcaccaccct
4381 ctgggggaaa aactgcctcc tcatatccaa cccaaacctc ccctgtctca gtgtaaagcc
4441 attccccctt gtcctatcaa gggggagttt gctgtgacat tgttggtctg gggtgacaca
4501 tgtttgccaa ttcagtgcat cacggagagg cagatcttgg ggataaggaa gtgcaggaca
4561 gcatggacgt gggacatgca ggtgttgagg gctctgggac actctccaag tcacagcgtt
4621 cagaacagcc ttaaggataa gaagatagga tagaaggaca aagagcaagt taaaacccag
4681 catggagagg agcacaaaaa ggccacagac actgctggtc cctgtgtctg agcctgcatg
4741 tttgatggtg tctggatgca agcagaaggg gtggaagagc ttgcctggag agatacagct
4801 gggtcagtag gactgggaca ggcagctgga gaattgccat gtagatgttc atacaatcgt
4861 caaatcatga aggctggaaa agccctccaa gatccccaag accaacccca acccacccac
4921 cgtgcccact ggccatgtcc ctcagtgcca catccccaca gttcttcatc acctccaggg
4981 acggtgaccc ccccacctcc gtgggcagct gtgccactgc agcaccgctc tttggagaag
5041 gtaaatcttg ctaaatccag cccgaccctc ccctggcaca acgtaaggcc attatctctc
5101 atccaactcc aggacggagt cagtgagaat attggcgcgc cgaattctcg cgacgatcgg
5161 tcgacataac ttcgtatagg atactttata cgaagttatc aattcaggct agttattaat
5221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
5281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
5341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
5401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
5461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
5521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
5581 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
5641 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
5701 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
5761 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
5821 ggccctataa aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc
5881 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
5941 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
6001 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
6061 tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
6121 gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc
6181 gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg
6241 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
6301 gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg
6361 cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg
6421 ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc
6481 gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
6541 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
6601 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
6661 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
6721 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
6781 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
6841 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
6901 gtctcatcat tttggcaaag aattgtgtac aaccatggag agcgacgaga gcggcctgcc
6961 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
7021 gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa
7081 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
7141 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
7201 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
7261 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc
7321 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
7381 gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga
7441 cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
7501 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag
7561 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc
7621 cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac
7681 cggatctcgc taaacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
7741 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
7801 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
7861 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
7921 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
7981 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
8041 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
8101 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
8161 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
8221 atggagatca ccggtataac ttcgtataat gtatactata cgaagttatg gtaccttaat
8281 taagttaaca tgcatagtac tgagctcacg gggacagccc ccccccaaag cccccaggga
8341 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
8401 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
8461 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
8521 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
8581 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
8641 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
8701 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
8761 tgggggaaaa actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca
8821 ttcccccttg tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat
8881 gtttgccaat tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag
8941 catggacgtg ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc
9001 agaacagcct taaggataag aagataggat agaaggacaa agagcaagtt aaaacccagc
9061 atggagagga gcacaaaaag gccacagaca ctgctggtcc ctgtgtctga gcctgcatgt
9121 ttgatggtgt ctggatgcaa gcagaagggg tggaagagct tgcctggaga gatacagctg
9181 ggtcagtagg actgggacag gcagctggag aattgccatg tagatgttca tacaatcgtc
9241 aaatcatgaa ggctggaaaa gccctccaag atccccaaga ccaaccccaa cccacccacc
9301 gtgcccactg gccatgtccc tcagtgccac atccccacag ttcttcatca cctccaggga
9361 cggtgacccc cccacctccg tgggcagctg tgccactgca gcaccgctct ttggagaagg
9421 taaatcttgc taaatccagc ccgaccctcc cctggcacaa cgtaaggcca ttatctctca
9481 tccaactcca ggacggagtc agtgagaata ttgtttaaac actagggaca ggattggtga
9541 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
9601 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
9661 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
9721 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
9781 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
9841 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
9901 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
9961 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
10021 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
10081 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
10141 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
10201 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
10261 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
10321 cctgcccctt ccctacaggg gttcctggct ctgctctgtt taaacactgg ccgtcgtttt
10381 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
10441 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
10501 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
10561 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
10621 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
10681 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
10741 gtcatcaccg aaacgcgcga
//
