| 质粒名称: | pEGFP-C1-AR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 28235 |
| 存储实验室: | Michael Mancini |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4731 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Androgen receptor |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2760 bp |
| 基因突变: | -- |
| GenBank编号 | NM_000044 |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（主干上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Kpn I(未破坏) |
| 3' 克隆位点: | BamHI（在克隆过程中被破坏） |
| 5' 测序引物 | EGFP-C |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-C1-AR was a gift from Michael Mancini (Addgene plasmid # 28235 ; http://n2t.net/addgene:28235 ; RRID:Addgene_28235) |
| 参考文献部分: | Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA. Hum Mol Genet. 1999 May . 8(5):731-41. 10.1093/hmg/8.5.731 PubMed 10196362 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEGFP-C1-AR 7942 bp ds-DNA circular SYN 20-DEC-2018 DEFINITION Mammalian expression of Androgen receptor fused to EGFP. ACCESSION . VERSION . KEYWORDS pEGFP-C1-AR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7942) AUTHORS Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA TITLE Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. JOURNAL Hum Mol Genet. 1999 May . 8(5):731-41. PUBMED 10196362 REFERENCE 2 (bases 1 to 7942) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 20, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7942 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 86..389 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 390..593 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 544..564 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 632..641 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 638..1354 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(683..704) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(944..963) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1291..1312 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 4755..4876 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4792..4811) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4846..4865 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4883..5338) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5020..5041) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5232..5251 /label=F1ori-R /note="F1 origin, reverse primer" promoter 5365..5469 /gene="bla" /label=AmpR promoter promoter 5471..5828 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5494..5514) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5679..5814 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5741..5760 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5863..6657 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5917..5936) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6527..6546 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6845..6864) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6889..6936 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 7265..7853 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7754..7773 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag 61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag 601 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct 661 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 721 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 781 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg 841 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc 901 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 961 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 1021 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 1081 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 1141 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 1201 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct 1261 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 1321 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc 1381 ttcgaattct gcagtcgacg gtaccatgga agtgcagtta gggctgggaa gggtctaccc 1441 tcggccgccg tccaagacct accgaggagc tttccagaat ctgttccaga gcgtgcgcga 1501 agtgatccag aacccgggcc ccaggcaccc agaggccgcg agcgcagcac ctcccggcgc 1561 cagtttgctg ctgctgcagc agcagcagca gcagcagcag cagcagcagc agcagcagca 1621 gcagcagcag cagcagcagc aagagactag ccccaggcag cagcagcagc agcagggtga 1681 ggatggttct ccccaagccc atcgtagagg ccccacaggc tacctggtcc tggatgagga 1741 acagcaacct tcacagccgc agtcggccct ggagtgccac cccgagagag gttgcgtccc 1801 agagcctgga gccgccgtgg ccgccagcaa ggggctgccg cagcagctgc cagcacctcc 1861 ggacgaggat gactcagctg ccccatccac gttgtccctg ctgggcccca ctttccccgg 1921 cttaagcagc tgctccgctg accttaaaga catcctgagc gaggccagca ccatgcaact 1981 ccttcagcaa cagcagcagg aagcagtatc cgaaggcagc agcagcggga gagcgaggga 2041 ggcctcgggg gctcccactt cctccaagga caattactta gggggcactt cgaccatttc 2101 tgacaacgcc aaggagttgt gtaaggcagt gtcggtgtcc atgggcctgg gtgtggaggc 2161 gttggagcat ctgagtccag gggaacagct tcggggggat tgcatgtacg ccccactttt 2221 gggagttcca cccgctgtgc gtcccactcc ttgtgcccca ttggccgaat gcaaaggttc 2281 tctgctagac gacagcgcag gcaagagcac tgaagatact gctgagtatt cccctttcaa 2341 gggaggttac accaaagggc tagaaggcga gagcctaggc tgctctggca gcgctgcagc 2401 agggagctcc gggacacttg aactgccgtc taccctgtct ctctacaagt ccggagcact 2461 ggacgaggca gctgcgtacc agagtcgcga ctactacaac tttccactgg ctctggccgg 2521 accgccgccc cctccgccgc ctccccatcc ccacgctcgc atcaagctgg agaacccgct 2581 ggactacggc agcgcctggg cggctgcggc ggcgcagtgc cgctatgggg acctggcgag 2641 cctgcatggc gcgggtgcag cgggacccgg ttctgggtca ccctcagccg ccgcttcctc 2701 atcctggcac actctcttca cagccgaaga aggccagttg tatggaccgt gtggtggtgg 2761 tgggggtggt ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg 2821 cgaggcggga gctgtagccc cctacggcta cactcggccc cctcaggggc tggcgggcca 2881 ggaaagcgac ttcaccgcac ctgatgtgtg gtaccctggc ggcatggtga gcagagtgcc 2941 ctatcccagt cccacttgtg tcaaaagcga aatgggcccc tggatggata gctactccgg 3001 accttacggg gacatgcgtt tggagactgc cagggaccat gttttgccca ttgactatta 3061 ctttccaccc cagaagacct gcctgatctg tggagatgaa gcttctgggt gtcactatgg 3121 agctctcaca tgtggaagct gcaaggtctt cttcaaaaga gccgctgaag ggaaacagaa 3181 gtacctgtgc gccagcagaa atgattgcac tattgataaa ttccgaagga aaaattgtcc 3241 atcttgtcgt cttcggaaat gttatgaagc agggatgact ctgggagccc ggaagctgaa 3301 gaaacttggt aatctgaaac tacaggagga aggagaggct tccagcacca ccagccccac 3361 tgaggagaca acccagaagc tgacagtgtc acacattgaa ggctatgaat gtcagcccat 3421 ctttctgaat gtcctggaag ccattgagcc aggtgtagtg tgtgctggac acgacaacaa 3481 ccagcccgac tcctttgcag ccttgctctc tagcctcaat gaactgggag agagacagct 3541 tgtacacgtg gtcaagtggg ccaaggcctt gcctggcttc cgcaacttac acgtggacga 3601 ccagatggct gtcattcagt actcctggat ggggctcatg gtgtttgcca tgggctggcg 3661 atccttcacc aatgtcaact ccaggatgct ctacttcgcc cctgatctgg ttttcaatga 3721 gtaccgcatg cacaagtccc ggatgtacag ccagtgtgtc cgaatgaggc acctctctca 3781 agagtttgga tggctccaaa tcacccccca ggaattcctg tgcatgaaag cactgctact 3841 cttcagcatt attccagtgg atgggctgaa aaatcaaaaa ttctttgatg aacttcgaat 3901 gaactacatc aaggaactcg atcgtatcat tgcatgcaaa agaaaaaatc ccacatcctg 3961 ctcaagacgc ttctaccagc tcaccaagct cctggactcc gtgcagccta ttgcgagaga 4021 gctgcatcag ttcacttttg acctgctaat caagtcacac atggtgagcg tggactttcc 4081 ggaaatgatg gcagagatca tctctgtgca agtgcccaag atcctttctg ggaaagtcaa 4141 gcccatctat ttccacaccc agtgaagcat tggaaaccct atttccccac cccagctcat 4201 gccccctttc agatgtcttc tgcctgttat aactctgcac tactcctctg cagtgccttg 4261 gggaatttcc tctattgatg tacagtctgt catgaacatg ttcctgaatt ctatttgctg 4321 ggcttttttt ttctctttct ctcctttctt tttcttcttc cctccctatc taaccctccc 4381 atggcacctt cagactttgc ttcccattgt ggctcctatc tgtgttttga atggtgttgt 4441 atgcctttaa atctgtgatg atcctcatat ggcccagtgt caagttgtgc ttgtttacag 4501 cactactctg tgccagccac acaaacgttt acttatctta tgccacggga agtttagaga 4561 gctaagatta tctggggaaa tcaaaacaaa aacaagcaaa caaaaaaaaa agaaaaacgg 4621 aattcagatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg 4681 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg 4741 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 4801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 4861 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta 4921 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 4981 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 5041 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 5101 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta 5161 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 5221 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 5281 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca 5341 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 5401 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 5461 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa 5521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 5581 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 5641 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 5701 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 5761 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 5821 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 5881 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 5941 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 6001 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg 6061 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 6121 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 6181 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 6241 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 6301 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 6361 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg 6421 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 6481 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 6541 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 6601 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 6661 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 6721 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 6781 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc 6841 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa 6901 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg 6961 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt 7021 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg 7081 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac 7141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 7201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 7261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 7321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 7381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 7441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 7501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 7561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 7621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 7681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 7741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 7801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 7861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 7921 ctgcgttatc ccctgattct gt //
