质粒名称: | pAtCas9-dead_1 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 91165 |
存储实验室: | Daniel Voytas |
载体骨架基本信息 |
|
载体名称: | pCAMBIA |
载体出品公司: | -- |
载体抗性: | 卡那霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 植物表达,CRISPR |
筛选标记: | 潮霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | AtCas9_D10A+H840A (dead) |
物种来源: | Synthetic |
插入大小: | -- |
基因突变: | D10A, H840A |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | pAtCas9-dead_1 was a gift from Daniel Voytas (Addgene plasmid # 91165 ; http://n2t.net/addgene:91165 ; RRID:Addgene_91165) |
参考文献部分: | A multi-purpose toolkit to enable advanced genome engineering in plants. Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF. Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. 10.1105/tpc.16.00922 PubMed 28522548 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAtCas9-dead_1 14215 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II. ACCESSION . VERSION . KEYWORDS pAtCas9-dead_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14215) AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF TITLE A multi-purpose toolkit to enable advanced genome engineering in plants. JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. PUBMED 28522548 REFERENCE 2 (bases 1 to 14215) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14215 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(291..1085) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 1510..1534 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 1612..1786 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(1827..2852) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" promoter complement(2919..3595) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" protein_bind 3786..3807 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3822..3852 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3860..3876 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3865..3887 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3884..3900 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3884..3900 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 4430..4775 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 4809..8912 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 8925..8945 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(9226..9243) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9226..9242) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9235..9257) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 9445..9469 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 10770..11399 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 11828..12901 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 12967..13161 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(13397..13419) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 13505..13645 /label=bom /note="basis of mobility region from pBR322" primer_bind 13560..13579 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(13660..13677) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13831..204) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13911..13930) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga 1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt 1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg 1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga 1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca 2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc 2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg 2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt 2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg 2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca 2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat 2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg 2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga 2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag 2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc 2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta 2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg 2761 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg 2821 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag 2881 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga 2941 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc 3001 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct 3061 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag 3121 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc 3181 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt 3241 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct 3301 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg 3361 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc 3421 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg 3481 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc 3541 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc 3601 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc 3661 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg 3721 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 3781 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 3841 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 3901 atgattacga attcgagctc ggtacccggg gatcggcgcg ccagatttgc cttttcaatt 3961 tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt atcatcaaga 4021 cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg 4081 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga 4141 tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa 4201 tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga 4261 ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc 4321 tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg 4381 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc 4441 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta 4501 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa 4561 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga 4621 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg 4681 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct 4741 ctatataagg aagttcattt catttggaga gaacacgggg gactcctgca ggtagatcgc 4801 tcgtcgacat ggataagaag tactctatcg gactcgctat cggaactaac tctgtgggat 4861 gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca 4921 ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat tctggtgaaa 4981 ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga agaaagaaca 5041 ggatctgcta cctccaagag atcttctcta acgagatggc taaagtggat gattcattct 5101 tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag aggcacccta 5161 tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact atctaccacc 5221 tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc tacctcgctc 5281 tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc aaccctgata 5341 actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag cttttcgaag 5401 agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct aggctctcta 5461 agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag aacggacttt 5521 tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct aacttcgatc 5581 tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat ctcgataacc 5641 tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag aacctctctg 5701 atgctatcct cctcagtgat atcctcagag tgaacaccga gatcaccaag gctccactct 5761 cagcttctat gatcaagaga tacgatgagc accaccagga tctcacactt ctcaaggctc 5821 ttgttagaca gcagctccca gagaagtaca aagagatttt cttcgatcag tctaagaacg 5881 gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc 5941 ctatcctcga gaagatggat ggaaccgagg aactcctcgt gaagctcaat agagaggatc 6001 ttctcagaaa gcagaggacc ttcgataacg gatctatccc tcatcagatc cacctcggag 6061 agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag gataacaggg 6121 aaaagattga gaagattctc accttcagaa tcccttacta cgtgggacct ctcgctagag 6181 gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc ccttggaact 6241 tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg atgaccaact 6301 tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc tacgagtact 6361 tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg aggaagcctg 6421 cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag accaacagaa 6481 aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc ttcgattcag 6541 ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac cacgatctcc 6601 tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat atcttggagg 6661 atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa aggctcaaga 6721 cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga agatacactg 6781 gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag tctggaaaga 6841 caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg cagctcatcc 6901 acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca ggacagggtg 6961 atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag aagggaatcc 7021 tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat aagcctgaga 7081 acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag aagaactcta 7141 gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag atcctcaaag 7201 agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac tacctccaga 7261 acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct gattacgatg 7321 ttgatgctat cgtgccacag tcattcttga aggatgattc tatcgataac aaggtgctca 7381 ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag gttgtgaaga 7441 aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag agaaagttcg 7501 ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca ggattcatca 7561 agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc ctcgattcta 7621 ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag gttatcaccc 7681 tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag gtgagggaaa 7741 tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga accgctctca 7801 tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag gtgtacgatg 7861 tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct aagtatttct 7921 tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac ggtgagatca 7981 gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg gataagggaa 8041 gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc gttaagaaaa 8101 ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg aactctgata 8161 agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc gattctccta 8221 ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt aagaagctca 8281 agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc gagaagaacc 8341 caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc atcatcaagc 8401 tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc gcttctgctg 8461 gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac tttctttacc 8521 tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag aagcaacttt 8581 tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct gagttctcta 8641 aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac aacaagcaca 8701 gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc cttaccaacc 8761 tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag agatacacct 8821 ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga ctctacgaga 8881 ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag aagaagagga 8941 aggtttgact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt 9001 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct 9061 aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct ataatccatt 9121 gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga 9181 ctatggaata tgattaaaga taaggagctc cggtgacgga ctggcactgg ccgtcgtttt 9241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 9301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 9361 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg 9421 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa 9481 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc 9541 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc 9601 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga 9661 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 9721 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 9781 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 9841 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 9901 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 9961 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 10021 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 10081 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 10141 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 10201 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 10261 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 10321 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 10381 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 10441 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 10501 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 10561 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 10621 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 10681 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 10741 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 10801 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 10861 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 10921 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 10981 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 11041 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 11101 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 11161 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 11221 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 11281 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 11341 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 11401 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 11461 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 11521 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 11581 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 11641 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 11701 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 11761 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg 11821 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 11881 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga 11941 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 12001 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 12061 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 12121 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 12181 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta 12241 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 12301 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 12361 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 12421 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 12481 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 12541 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 12601 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 12661 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 12721 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 12781 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 12841 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 12901 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 12961 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 13021 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 13081 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 13141 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 13201 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 13261 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 13321 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg 13381 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 13441 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 13501 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 13561 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 13621 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 13681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 13741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 13801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 13861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 13921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 13981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 14041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 14101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 14161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtat //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pEGFP-C1-AR
- 下一篇:RCASBP-AP (A) (CC#37)