| 质粒名称: | pSKI074 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11571 |
| 存储实验室: | Detlef Weigel |
|
载体骨架基本信息 |
|
| 载体名称: | pPCVICEn4HPT |
| 载体出品公司: | Hayashi et al, 1992 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10138 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达；激活标记 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CaMV 35S enhancers |
| 物种来源: | Cauliflower mosaic virus |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | AF218466 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | See map and paper.(未破坏) |
| 3' 克隆位点: | See map and paper. (未破坏) |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSKI074 was a gift from Detlef Weigel (Addgene plasmid # 11571 ; http://n2t.net/addgene:11571 ; RRID:Addgene_11571) |
| 参考文献部分: | Activation tagging in Arabidopsis. Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM, Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ, Yanofsky MF, Chory J. Plant Physiol. 2000 Apr . 122(4):1003-13. 10.1104/pp.122.4.1003 PubMed 10759496 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSKI074 10450 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSKI074
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10450)
AUTHORS Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK,
Fankhauser C, Ferrandiz C, Kardailsky I, Malancharuvil EJ, Neff MM,
Nguyen JT, Sato S, Wang ZY, Xia Y, Dixon RA, Harrison MJ, Lamb CJ,
Yanofsky MF, Chory J
TITLE Activation tagging in Arabidopsis.
JOURNAL Plant Physiol. 2000 Apr . 122(4):1003-13.
PUBMED 10759496
REFERENCE 2 (bases 1 to 10450)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10450
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(13..31)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(52..68)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(52..68)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(65..87)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 76..92
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(100..130)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 145..166
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(283..300)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(454..1042)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(534..553)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1213..2073)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1836..1855
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2074..2178)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2207..2662)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2344..2365)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2556..2575
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2792..2814
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2806..2823
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2807..2823
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2830..2849
/label=T7
/note="T7 promoter, forward primer"
promoter 2830..2848
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 2881..2897
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2881..2897
/label=pBluescriptSK
/note="For pBluescript vector"
misc_feature 4270..4294
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
oriT 5497..5606
/note="incP origin of transfer"
rep_origin 6401..7113
/label=oriV
/note="incP origin of replication"
misc_feature 7700..7724
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
primer_bind complement(8045..8061)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8046..8062)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter 8362..8741
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
CDS 8771..9565
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8825..8844)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9435..9454
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
ORIGIN
1 cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat ggtcatagct
61 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
1021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
1081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
1141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
1201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
1261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
1321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
1381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
1441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
1501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
1561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
1621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
1681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
1741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
1801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
1861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
1921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
1981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
2041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
2101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
2161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggaaat tgtaaacgtt
2221 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
2281 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
2341 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
2401 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
2461 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
2521 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
2581 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
2641 aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa
2701 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
2761 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
2821 agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc
2881 tctagaacta gtggatcccc aacatggtgg agcacgacac tctcgtctac tccaagaata
2941 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
3001 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
3061 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
3121 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
3181 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tagatcccca
3241 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
3301 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
3361 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
3421 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
3481 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
3541 cttcaaagca agtggattga tgtgatatct agatccccaa catggtggag cacgacactc
3601 tcgtctactc caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt
3661 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
3721 tcatcgaaag gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag
3781 gaaaggctat cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca
3841 cgaggaacat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
3901 gtgatatcta gatccccaac atggtggagc acgacactct cgtctactcc aagaatatca
3961 aagatacagt ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg
4021 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
4081 aggaagatgg cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
4141 cctctaccga cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag
4201 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctag atccgaaact
4261 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
4321 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
4381 ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca
4441 accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc
4501 gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg
4561 gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat
4621 ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac
4681 aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc
4741 cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc
4801 gccgccgacg ccgttgatgt ggacgccggg cccgatcata ttgtcgctca ggatcgtggc
4861 gttgtgcttg tcggccgttg ctgtcgtaat gatatcggca ccttcgaccg cctgttccgc
4921 agaggtgcag gcctcgatct gaaacccgaa ccgctggaga ttgcgggagc agcgagcagt
4981 agcctcgggg tcgatgtcgt aaagtcgtat ccgatcgacg ccgatcagcg ccttgaaggc
5041 caaagcctgg aactcacttt gggcaccgtt gccgatcagc gccatcgtgc gcgaatcttt
5101 acgggccaga tactttgccg cgatcgcgga ggtcgcggcc gttcgcaagg ccgtcaggat
5161 tgtcatttcc gacagcagca gcggatagcc gctatcgaca tcggagagca cgccgaacgc
5221 ggttaccggc ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct
5281 ggcggacggg aagtatccag ctcgagatcc ggattttgta gccctggccg acggccagca
5341 ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt
5401 ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
5461 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
5521 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
5581 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
5641 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
5701 tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc cctgctgttt
5761 tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac tgaggggaca
5821 ggcgagagac gatgccaaag agctacaccg acgagctggc cgagtgggtt gaatcccgcg
5881 cggccaagaa gcgccggcgt gatgaggctg cggttgcgtt cctggcggtg agggcggatg
5941 tcgaggcggc gttagcgtcc ggctatgcgc tcgtcaccat ttgggagcac atgcgggaaa
6001 cggggaaggt caagttctcc tacgagacgt tccgctcgca cgccaggcgg cacatcatan
6061 gccaagcccg ccgatgtgcc cgcaccgcag gccaaggctg cggaacccgc gccggcaccc
6121 aagacgccgg agccacggcg gccgaagcag gggggcaagg ctgaaaagcc ggcccccgct
6181 gcggccccga ccggcttcac nttcaaccca acaccggaca aaaaggatct agcgtggact
6241 caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg ggcaccgcag
6301 ggaaattctn gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt
6361 ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca gcgcctttcc gacgctcacc
6421 gggctggttg ccctcgccgc tgggctggcg gccgttctat ggccctgcaa acgcgccaga
6481 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgnggat acctngcgga
6541 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
6601 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
6661 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
6721 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
6781 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
6841 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
6901 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
6961 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
7021 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
7081 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgnc nnngcnngga
7141 tcggggcnnt nnnggnatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc
7201 cgcaggtgct ggcatcnaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg
7261 gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg
7321 caatttttac cttgggcatt cttggcatan tggtcgcggg tgccgtgctc gtgttcgggg
7381 gtgaattgca agctagcttg cttggtcgtt ccggtaccgt gaacgtcggc tcgattgtac
7441 ctgcgttcaa atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac
7501 ggatcgactg cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga
7561 tcactccgtt gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca
7621 atggaaatta tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc
7681 gggtgcaaag cggcagcggc ggcaggatat attcaattgt aaatggcttc atgtccggga
7741 aatctacatg gatcagcaat gagtatgatg gtcaatatgg aggaaaagaa agagtaatta
7801 ccaatttttt ttcaattcaa aaatgtagat gtccgcagcg ttattataaa atgaaagtac
7861 attttgataa aacgacaaat tacgatccgt cgtatttata ggcgaaagca ataaacaaat
7921 tattctaatt cggaaatctt tatttcgacg tgtctacatt cacgtccaaa tgggggctta
7981 gatgagaaac ttcacgatcg atatctagat ctcgagctcg agatctagat atcgataagc
8041 ttatcgatac cgtcgacctc gaggggccaa cagagcctgg cgttcccctt ttgcattgag
8101 accgatgttc gttccggaac cttgcacgcc ccagagcttc tcaccgttca cgacaatttc
8161 cttctcgttg aggtcggtcg cgccatgtcg gatgaaataa aaacttttga taccagcggg
8221 ggccttcgca gagccgaggt aggtctgaga aattggcatt ttcacgtgtg gaagatatga
8281 atttttttga gaaactagat aagattaatg aatatcggtg ttttggtttt ttcttgtggc
8341 cgtctttgtt tatattgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatctga
8401 gctagttttt atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc
8461 gggcctgaat ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt
8521 aacgactttt gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac
8581 aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt
8641 cagaaatgga taaatagcct tgcttcctat tatatcttcc caaataccaa tacattacac
8701 tagcatctga atttcataac caatctcgat acaccaaatc gactctagcg aattccccgg
8761 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
8821 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
8881 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
8941 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
9001 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
9061 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
9121 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
9181 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
9241 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
9301 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
9361 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
9421 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
9481 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
9541 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
9601 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
9661 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
9721 gagttcttcg cccaccccgg aattccagct tccctgaaac cttggactcc catgttggca
9781 aaggcaacca aacaaacaag aagatccgct cctgcatatg gggcggtttg agtatttcaa
9841 ctgccatttg ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat
9901 attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt
9961 catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat cgatagtact
10021 acgaatattt cgtatcgctg atcttctcaa tcacaatgat gcgtagtgac ccgacaaata
10081 atttaagcgt ccttaatacc aatcctaaaa taattgaggc aaataaaatt tttttgtaat
10141 ttttatgata gcagatcgat tctccagcaa gcctgcaaca aaatattgtg tatttctaaa
10201 tagattttga tattaaaatc ccgagaaagc aaaattgcat ttaacaaaac agtaatttag
10261 tacattaata aaaattatgc tcaaacattt ccatgaaatt aaaacataat tacattttac
10321 aacacaatat tttgcgatat tattgccttt cgccaattta acactggaag caattaaagt
10381 aggcccacac agagcgcttt gcaacactga caacgcttgc ggttggtcgg ctcgaggggg
10441 ggcccggtac
//
