| 质粒名称: | pUC-UAS1B8-TEF(136) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 44380 |
| 存储实验室: | Hal Alper |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 空载体大小: | 2623 bp |
| 完整质粒大小: | 3686 bp |
| 载体类型: | Bacterial Expression, Synthetic Biology |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH10B |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | UAS1B8-TEF(136) promoter |
| 物种来源: | Yarrowia lipolytica |
| 插入大小: | 1063bp |
| 基因突变: | N/A |
| GenBank编号 | JN247409 |
| 启动子: | UAS1B8-TEF(136) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BstBI(未破坏) |
| 3’克隆位点: | AscI (未破坏) |
| 5’测序引物 | m13_forward20_primer |
| 3’测序引物 | m13_reverse_primer |
|
文献引用方法 |
|
| 材料和方法部分: | pUC-UAS1B8-TEF(136) was a gift from Hal Alper (Addgene plasmid # 44380 ; http://n2t.net/addgene:44380 ; RRID:Addgene_44380) |
| 参考文献部分: | Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Blazeck J, Liu L, Redden H, Alper H. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16. 10.1128/AEM.05763-11 PubMed 21926196 |
买家导航
pUC-UAS1B8-TEF载体基本信息
pUC-UAS1B8-TEF载体图谱质粒图谱和多克隆位点信息
44380质粒图谱.png)
pUC-UAS1B8-TEF载体简介
pUC-UAS1B8-TEF载体序列
LOCUS pUC-UAS1B8-TEF(136) 3704 bp ds-DNA circular SYN 09-AUG-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pUC-UAS1B8-TEF(136)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3704)
AUTHORS Blazeck J, Liu L, Redden H, Alper H
TITLE Tuning gene expression in Yarrowia lipolytica by a hybrid promoter
approach.
JOURNAL Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi:
10.1128/AEM.05763-11. Epub 2011 Sep 16.
PUBMED 21926196
REFERENCE 2 (bases 1 to 3704)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 9, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3704
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(29..51)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 151..170
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 364..386
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 378..395
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1483..1499)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1483..1499)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1496..1518)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1507..1523
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1531..1561)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1576..1597
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1714..1731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1885..2473)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1965..1984)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2644..3504)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3267..3286
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3505..3609)
/gene="bla"
/label=AmpR promoter
primer_bind 3677..3695
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtttcga aggtaccaag gaagcatgcg
421 gtacccgaat tcctgaggtg tctcacaagt gccgtgcagt cccgccccca cttgcttctc
481 tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc cgcccacctc gatccggtct
541 agactgaggt gtctcacaag tgccgtgcag tcccgccccc acttgcttct ctttgtgtgt
601 agtgtacgta cattatcgag accgttgttc ccgcccacct cgatccgggg atccctgagg
661 tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt
721 acattatcga gaccgttgtt cccgcccacc tcgatccggg tcgacctgag gtgtctcaca
781 agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg tacattatcg
841 agaccgttgt tcccgcccac ctcgatccgg gagctcctga ggtgtctcac aagtgccgtg
901 cagtcccgcc cccacttgct tctctttgtg tgtagtgtac gtacattatc gagaccgttg
961 ttcccgccca cctcgatccg gtctagactg aggtgtctca caagtgccgt gcagtcccgc
1021 ccccacttgc ttctctttgt gtgtagtgta cgtacattat cgagaccgtt gttcccgccc
1081 acctcgatcc ggggatccct gaggtgtctc acaagtgccg tgcagtcccg cccccacttg
1141 cttctctttg tgtgtagtgt acgtacatta tcgagaccgt tgttcccgcc cacctcgatc
1201 cgggtcgacc tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt gcttctcttt
1261 gtgtgtagtg tacgtacatt atcgagaccg ttgttcccgc ccacctcgat ccgggcatgc
1321 ctgcagaagc ttttgtggtt gggactttag ccaagggtat aaaagaccac cgtccccgaa
1381 ttacctttcc tcttcttttc tctctctcct tgtcaactca cacccgaaat cgttaagcat
1441 ttccttctga gtataagaat cattcaaagg cgcgccatca tggtcatagc tgtttcctgt
1501 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
1561 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
1621 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
1681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
1741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
1801 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
1861 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
1921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
1981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
2041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
2101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
2161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
2221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
2281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
2341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
2401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
2461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
2521 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
2581 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
2641 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
2701 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
2761 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
2821 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
2881 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
2941 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
3001 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
3061 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
3121 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
3181 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
3241 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
3301 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
3361 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
3421 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
3481 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
3541 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
3601 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
3661 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
