pEYK3.1

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pEYK3.1 
出品公司: 爱迪基因
目录编号: 17366
存储实验室: George Daley

载体骨架基本信息
载体名称: pEYK3.1
载体出品公司: --
载体抗性: 博来霉素
空载体大小: 3422 bp
完整质粒大小: --
载体修饰: --
载体类型: 逆转录病毒
筛选标记: 博来霉素
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: GFP
物种来源: --
插入大小: 719 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: pEYK3.1 was a gift from George Daley (Addgene plasmid # 17366 ; http://n2t.net/addgene:17366 ; RRID:Addgene_17366)
参考文献部分: Novel retroviral vectors to facilitate expression screens in mammalian cells. Koh EY, Chen T, Daley GQ. Nucleic Acids Res. 2002 Dec 15. 30(24):e142. 10.1093/nar/gnf142 PubMed 12490733

载体图谱质粒图谱和多克隆位点信息

pEYK3.1质粒图谱

载体简介

载体序列

LOCUS       pEYK3.1           4139 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEYK3.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Koh EY, Chen T, Daley GQ
  TITLE     Novel retroviral vectors to facilitate expression screens in 
            mammalian cells.
  JOURNAL   Nucleic Acids Res. 2002 Dec 15. 30(24):e142.
  PUBMED    12490733
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    complement(579..612)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      complement(858..1446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(938..957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1479..1853)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1872..1919)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(2088..2807)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2133..2154)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2482..2501
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2741..2762
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2804..2813
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    2853..3227
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2899..2915)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(3237..3653)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3253..3269)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3285..3307)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3718..4075
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
       61 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
      121 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
      181 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
      241 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
      301 ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
      361 gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc ttggccctga
      421 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
      481 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
      541 aaatggcgtt acttaagcta gaggcgcgcc ttaattaaat aacttcgtat aatgtatgct
      601 atacgaagtt atgcggccgc gctagcttgc caaacctaca ggtggggtct ttcattcccc
      661 cctttttctg gagactaaat aaaatctttt attttatcta tggctcgtac tctataggct
      721 tcagctggtg atattgttga gtcaaaacta gagcctggac cactgatatc ctgtctttaa
      781 caaattggac taatcgatat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1441 tctcaagaag atcctttgat cttttctacg ggacgcgttc agtcctgctc ctcggccacg
     1501 aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg
     1561 ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac
     1621 cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc
     1681 accacctggt cctggaccgc gctgatgaac agagtcacgt cgtcccggac cacaccggcg
     1741 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
     1801 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag
     1861 ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca
     1921 gtcgaccact gtgctggcgg ccggccgctc gagtttaaat agtacataat ggatttcctt
     1981 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
     2041 tggtaatggt agcgaccggc gctcagctgg aattcttaat taattcatta cttgtacagc
     2101 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
     2161 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
     2221 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
     2281 ctgccgtcct cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg
     2341 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
     2401 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
     2461 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
     2521 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
     2581 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
     2641 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
     2701 ccctcgccct cgccagacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     2761 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgttaatt
     2821 aagaattccc gtaccaccac actgggatcc ggcagtctag aggatggtcc acccccgggg
     2881 tcggcagcct tcacgtgggc ggcgtgtatc caagctgcga tgccgtctac tttgagggcg
     2941 gtgggggtgg tcagcaggac tgtgtaaggt cctttccagc gaggttctag gttcttagtc
     3001 tggtgtcggc ggacccacac tgtgtcgccg actcggtaag ggtgaggtac caccggtcgg
     3061 tccagttgtt cttggtaggc tgccgccaga ggtctccaga cttcgtgctg gactaagtag
     3121 agagcctgta agtgagcttg gagagagggg ctgttagtaa ctcttgtctt gtcagggtca
     3181 gggaagttta caaggggcgg gggtgcccca tataagatct catatggcca tatgggggcg
     3241 cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt
     3301 tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag
     3361 ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc
     3421 tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg
     3481 tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag
     3541 cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg
     3601 acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg gcccgggccc
     3661 ttattttcag acaaatacag aaacacagtc agacagagac aacacagaac gatgctgcag
     3721 cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa gcaaaaattc agacggaggc
     3781 gggaactgtt ttaggttctc gtctcctacc agaaccacat atccctcctc taaggggggt
     3841 gcaccaaaga gtccaaaacg atcgggattt ttggactcag gtcgggccac aaaaacggcc
     3901 cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt gttccgaact cgtcagttcc
     3961 accacgggtc cgccagatac agagctagtt agctaactag taccgacgca ggcgcataaa
     4021 atcagtcata gacactagac aatcggacag acacagataa gttgctggcc agcttacctc
     4081 ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga gcccccaaa
//

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