| 质粒名称: | p1585588 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 62620 |
| 存储实验室: | Mattheos Koffas |
|
载体骨架基本信息 |
|
| 载体名称: | pETM6 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5203 bp |
| 完整质粒大小: | 9155 bp |
| 载体类型: | Bacterial Expression, Synthetic Biology |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | flavaone 3B-hydroxylase |
| 物种来源: | Camellia sinensis |
| 插入大小: | 1200 bp |
| 基因又名: | CsF3H |
| GenBank编号 | |
| 启动子 | T7 |
| 标签/融合蛋白: | GBD (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NdeI(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | gcaccgaccaccaccctgac |
| 3' 测序引物 | cctcatcttcatcaccggc |
|
文献引用方法 |
|
| 材料和方法部分: | p1585588 was a gift from Mattheos Koffas (爱迪基因 plasmid # 62620) |
| 参考文献部分: | Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S, Wang Z, Koffas MA. Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002. 10.1016/j.ymben.2014.12.002 PubMed 25527438 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p1585588 14761 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3
Ratio).
ACCESSION .
VERSION .
KEYWORDS p1585588
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14761)
AUTHORS Zhao S, Jones JA, Lachance DM, Bhan N, Khalidi O, Venkataraman S,
Wang Z, Koffas MA
TITLE Improvement of catechin production in Escherichia coli through
combinatorial metabolic engineering.
JOURNAL Metab Eng. 2014 Dec 17;28C:43-53. doi: 10.1016/j.ymben.2014.12.002.
PUBMED 25527438
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|p1585588|
FEATURES Location/Qualifiers
source 1..14761
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 3..27
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 42..64
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 219..230
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 1287..1331
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 1384..1431
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 1465..1483
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1484..1508
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 1523..1545
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 2642..2686
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 2739..2786
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 2820..2838
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 2839..2863
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 2878..2900
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 4090..4134
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 4187..4234
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 4268..4286
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 4287..4311
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 4326..4348
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5445..5489
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 5542..5589
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 5623..5641
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5642..5666
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5681..5703
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 6800..6844
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 6897..6944
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 6978..6996
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 6997..7021
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 7036..7058
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 8248..8292
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 8345..8392
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 8426..8444
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 8445..8469
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 8484..8506
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 9696..9740
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
terminator 9793..9840
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 9884..10339
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9971..9990)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10181..10202
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(10454..11314)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11077..11096
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11315..11407)
/gene="bla"
/label=AmpR promoter
rep_origin 11488..12076
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11977..11996
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12230..12247
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 12262..12404
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(12330..12349)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 12490..12512
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(12506..12697)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(13269..14351)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 14313..14332
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(14352..14429)
/gene="lacI"
/label=lacI promoter
primer_bind 14635..14654
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 14745..2
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
61 gagatataca tatggcaccg accaccaccc tgaccgcact ggcagaagaa aaaagcctgc
121 agcagaaatt tgttcgtgat gaagatgaac gtccgaaagt tgcctataat gtgtttagca
181 atgaaatccc ggttattagc ctggcaggta ttgatgaaat tgaaggtcgt cgtagcgaaa
241 tttgccgtaa aattgttgaa gcatgtgaag gttggggtgt ttttcaggtt gttgatcatg
301 gtgttgatgc aaatctgatt gcagaaatga cccgtctggc acgtgaattt tttgcactgc
361 ctccggaaga aaaactgcgt tttgatatga gcggtggtaa aaaaggtggt tttattgtta
421 gcagccatct gcagggtgaa gcagttcagg attggcgtga aattgttacc tatttcagct
481 atccgattcg tgcacgtgat tatagccgtt ggcctgataa accggaaggt tggcgtgcag
541 ttaccgaaac ctatagcgaa aaactgatgg atctggcatg taaactgctg gaagttctga
601 gcgaagcaat gggtctggaa aaagaggcac tgaccaaagc atgtgttgat atggatcaga
661 aagtggtgat caacttctat ccgaaatgtc cgcagccgga tctgaccctg ggtctgaaac
721 gtcataccga tccgggtaca attaccctgc tgctgcaaga tcaggtgggt ggtctgcagg
781 caacccgtga tggtggcaaa acctggatta ccgttcagcc ggttgaaggt gcatttgttg
841 ttaatctggg tgatcatggc cattatctga gcaatggtcg ctttaaaaac gcagatcatc
901 aggcagttgt taatagcaat tgtagccgtc tgagcattgc aacctttcag aatccggcac
961 cggaagcaac cgtttatccg ctgaaaattc gtgaaggtga aaaaccgatt ctggaagaac
1021 cgattacctt tgccgatatg tataaacgca aaatgagcaa agatatcgag ctggccaaac
1081 tgaaaaaact ggcgaaagaa aaaaaactgc tgcaagacca gcaggatatc gaaaaagcaa
1141 aactggaaat caaaagcacc gatgaaatct tcgccctggt tggtgcactg atgcatgtta
1201 tgcagaaacg tagccgtgca attcatagca gtgatgaagg tgaagatcaa gccggtgatg
1261 aagatgagga ttaactcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc
1321 acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa
1381 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctaggatcg
1441 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
1501 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatgaaaga
1561 tagcgttgca agcgcaaccg caagcgcacc gggtacagtt tgtgttaccg gtgcagcagg
1621 ttttattggt agctggctgg ttatgcgtct gctggaacgt ggttatattg ttcgtgcaac
1681 cgttcgtgat ccggcaaatc tgaaaaaagt taaacatctg ctggatctgc cgaaagcaga
1741 taccaatctg accctgtgga aagccgatct gaatgaagag ggtagctttg atgaagcaat
1801 tgaaggttgt agcggtgttt ttcatgttgc aaccccgatg gattttgaaa gcaaagatcc
1861 ggaaaacgaa gtgattaaac cgaccattaa cggtgtgctg agcattattc gtagctgtac
1921 caaagcaaaa accgttaaac gtctggtttt taccagcagc gcaggtacag ttaatgttca
1981 agaacatcag cagccggtgt ttgatgaaaa caattggagc gatctgcact tcatcaacaa
2041 aaaaaaaatg accggctgga tgtattttgt gagcaaaacc ctggcagaaa aagcagcatg
2101 ggaagcagca aaagaaaaca acattgattt catcagcatt atcccgaccc tggttggtgg
2161 tccgtttatt atgccgacct ttccgcctag cctgattacc gcactgagcc cgattacccg
2221 taatgaaggt cattattcca ttatcaaaca gggccagttt gtgcatctgg atgatctgtg
2281 tgaaagccac atttttctgt atgaacgtcc gcaggcagaa ggtcgttata tttgtagcag
2341 ccatgatgca accattcatg atctggccaa actgatgcgt gaaaaatggc ctgaatataa
2401 tgttccgacc gaattcaaag gcatcgataa agatctgccg gttgttagct tttccagcaa
2461 aaaactgatt ggcatgggct tcgagttcaa atatagcctg gaagatatgt ttcgtggtgc
2521 cattgatacc tgtcgtgaaa aaggtctgct gccgcatagc tttgcagaaa atccggttaa
2581 tggcaacaaa gtgcctcctc ctgcactgcc tccgaaacgt cgtcgttaac tcgagtctgg
2641 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
2701 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
2761 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
2821 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
2881 gtttaacttt aagaaggaga tatacatatg accgttagcg gtgcaattcc gagcatgacc
2941 aaaaatcgta ccctggttgt tggtggcacc ggttttattg gtcagtttat taccaaagca
3001 agcctgggtt ttggttatcc gacctttctg ctggttcgtc cgggtccggt tagcccgagc
3061 aaagcagtta ttatcaaaac ctttcaggat aaaggtgcca aagtgattta tggcgtgatc
3121 aacgataaag aatgcatgga aaaaattctg aaagagtacg agatcgacgt tgttattagc
3181 ctggtgggtg gtgcacgtct gctggatcag ctgaccctgc tggaagcaat taaaagcgtt
3241 aaaaccatca aacgttttct gccgagcgaa tttggccatg atgttgatcg taccgatccg
3301 gttgaaccgg gtctgaccat gtataaagaa aaacgtctgg tgcgtcgtgc cgttgaagaa
3361 tatggtattc cgtttaccaa tatctgctgc aatagcattg caagctggcc gtattatgat
3421 aattgtcatc cgagccaggt tccgcctccg atggatcagt ttcagattta tggtgatggt
3481 aacaccaaag cctatttcat tgatggcaac gatatcggca aatttaccat gaaaaccatc
3541 gatgatattc gcaccctgaa caaaaatgtt cattttcgtc cgagcagcaa ctgctacagc
3601 attaatgaac tggcaagcct gtgggagaaa aaaatcggtc gtacactgcc tcgttttacc
3661 gttaccgcag ataaactgct ggcacatgca gcagaaaaca ttattccgga aagcattgtt
3721 agcagcttta cccacgatat ctttattaac ggttgccagg tgaactttag catcgatgaa
3781 catagtgatg tggaaatcga tacactgtat ccggatgaaa aatttcgtag cctggatgat
3841 tgctatgaag attttgttcc gatggtgcac gataaaattc atgcaggtaa aagcggtgaa
3901 atcaaaatca aagatggtaa accgctggtt cagaccggca ccattgaaga aattaacaaa
3961 gacattaaaa ccctggtgga aacccagccg aatgaagaga tcaaaaaaga tatgaaagca
4021 ctggttgaag ccgttccgat tagcgcaatg ggtggtgtta aagaaagcct ggtttaactc
4081 gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact
4141 agtcgcagct taattaacct aaactgctgc caccgctgag caataactag cataacccct
4201 tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga tctcgatccc
4261 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
4321 ataattttgt ttaactttaa gaaggagata tacatatgaa agatagcgtt gcaagcgcaa
4381 ccgcaagcgc accgggtaca gtttgtgtta ccggtgcagc aggttttatt ggtagctggc
4441 tggttatgcg tctgctggaa cgtggttata ttgttcgtgc aaccgttcgt gatccggcaa
4501 atctgaaaaa agttaaacat ctgctggatc tgccgaaagc agataccaat ctgaccctgt
4561 ggaaagccga tctgaatgaa gagggtagct ttgatgaagc aattgaaggt tgtagcggtg
4621 tttttcatgt tgcaaccccg atggattttg aaagcaaaga tccggaaaac gaagtgatta
4681 aaccgaccat taacggtgtg ctgagcatta ttcgtagctg taccaaagca aaaaccgtta
4741 aacgtctggt ttttaccagc agcgcaggta cagttaatgt tcaagaacat cagcagccgg
4801 tgtttgatga aaacaattgg agcgatctgc acttcatcaa caaaaaaaaa atgaccggct
4861 ggatgtattt tgtgagcaaa accctggcag aaaaagcagc atgggaagca gcaaaagaaa
4921 acaacattga tttcatcagc attatcccga ccctggttgg tggtccgttt attatgccga
4981 cctttccgcc tagcctgatt accgcactga gcccgattac ccgtaatgaa ggtcattatt
5041 ccattatcaa acagggccag tttgtgcatc tggatgatct gtgtgaaagc cacatttttc
5101 tgtatgaacg tccgcaggca gaaggtcgtt atatttgtag cagccatgat gcaaccattc
5161 atgatctggc caaactgatg cgtgaaaaat ggcctgaata taatgttccg accgaattca
5221 aaggcatcga taaagatctg ccggttgtta gcttttccag caaaaaactg attggcatgg
5281 gcttcgagtt caaatatagc ctggaagata tgtttcgtgg tgccattgat acctgtcgtg
5341 aaaaaggtct gctgccgcat agctttgcag aaaatccggt taatggcaac aaagtgcctc
5401 ctcctgcact gcctccgaaa cgtcgtcgtt aactcgagtc tggtaaagaa accgctgctg
5461 cgaaatttga acgccagcac atggactcgt ctactagtcg cagcttaatt aacctaaact
5521 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
5581 ggttttttgc taggatcgag atcgatctcg atcccgcgaa attaatacga ctcactatag
5641 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
5701 agatatacat atgaaagata gcgttgcaag cgcaaccgca agcgcaccgg gtacagtttg
5761 tgttaccggt gcagcaggtt ttattggtag ctggctggtt atgcgtctgc tggaacgtgg
5821 ttatattgtt cgtgcaaccg ttcgtgatcc ggcaaatctg aaaaaagtta aacatctgct
5881 ggatctgccg aaagcagata ccaatctgac cctgtggaaa gccgatctga atgaagaggg
5941 tagctttgat gaagcaattg aaggttgtag cggtgttttt catgttgcaa ccccgatgga
6001 ttttgaaagc aaagatccgg aaaacgaagt gattaaaccg accattaacg gtgtgctgag
6061 cattattcgt agctgtacca aagcaaaaac cgttaaacgt ctggttttta ccagcagcgc
6121 aggtacagtt aatgttcaag aacatcagca gccggtgttt gatgaaaaca attggagcga
6181 tctgcacttc atcaacaaaa aaaaaatgac cggctggatg tattttgtga gcaaaaccct
6241 ggcagaaaaa gcagcatggg aagcagcaaa agaaaacaac attgatttca tcagcattat
6301 cccgaccctg gttggtggtc cgtttattat gccgaccttt ccgcctagcc tgattaccgc
6361 actgagcccg attacccgta atgaaggtca ttattccatt atcaaacagg gccagtttgt
6421 gcatctggat gatctgtgtg aaagccacat ttttctgtat gaacgtccgc aggcagaagg
6481 tcgttatatt tgtagcagcc atgatgcaac cattcatgat ctggccaaac tgatgcgtga
6541 aaaatggcct gaatataatg ttccgaccga attcaaaggc atcgataaag atctgccggt
6601 tgttagcttt tccagcaaaa aactgattgg catgggcttc gagttcaaat atagcctgga
6661 agatatgttt cgtggtgcca ttgatacctg tcgtgaaaaa ggtctgctgc cgcatagctt
6721 tgcagaaaat ccggttaatg gcaacaaagt gcctcctcct gcactgcctc cgaaacgtcg
6781 tcgttaactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga
6841 ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag caataactag
6901 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga
6961 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
7021 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgac cgttagcggt
7081 gcaattccga gcatgaccaa aaatcgtacc ctggttgttg gtggcaccgg ttttattggt
7141 cagtttatta ccaaagcaag cctgggtttt ggttatccga cctttctgct ggttcgtccg
7201 ggtccggtta gcccgagcaa agcagttatt atcaaaacct ttcaggataa aggtgccaaa
7261 gtgatttatg gcgtgatcaa cgataaagaa tgcatggaaa aaattctgaa agagtacgag
7321 atcgacgttg ttattagcct ggtgggtggt gcacgtctgc tggatcagct gaccctgctg
7381 gaagcaatta aaagcgttaa aaccatcaaa cgttttctgc cgagcgaatt tggccatgat
7441 gttgatcgta ccgatccggt tgaaccgggt ctgaccatgt ataaagaaaa acgtctggtg
7501 cgtcgtgccg ttgaagaata tggtattccg tttaccaata tctgctgcaa tagcattgca
7561 agctggccgt attatgataa ttgtcatccg agccaggttc cgcctccgat ggatcagttt
7621 cagatttatg gtgatggtaa caccaaagcc tatttcattg atggcaacga tatcggcaaa
7681 tttaccatga aaaccatcga tgatattcgc accctgaaca aaaatgttca ttttcgtccg
7741 agcagcaact gctacagcat taatgaactg gcaagcctgt gggagaaaaa aatcggtcgt
7801 acactgcctc gttttaccgt taccgcagat aaactgctgg cacatgcagc agaaaacatt
7861 attccggaaa gcattgttag cagctttacc cacgatatct ttattaacgg ttgccaggtg
7921 aactttagca tcgatgaaca tagtgatgtg gaaatcgata cactgtatcc ggatgaaaaa
7981 tttcgtagcc tggatgattg ctatgaagat tttgttccga tggtgcacga taaaattcat
8041 gcaggtaaaa gcggtgaaat caaaatcaaa gatggtaaac cgctggttca gaccggcacc
8101 attgaagaaa ttaacaaaga cattaaaacc ctggtggaaa cccagccgaa tgaagagatc
8161 aaaaaagata tgaaagcact ggttgaagcc gttccgatta gcgcaatggg tggtgttaaa
8221 gaaagcctgg tttaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
8281 cacatggact cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca
8341 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc
8401 gagatcgatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
8461 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgaccg
8521 ttagcggtgc aattccgagc atgaccaaaa atcgtaccct ggttgttggt ggcaccggtt
8581 ttattggtca gtttattacc aaagcaagcc tgggttttgg ttatccgacc tttctgctgg
8641 ttcgtccggg tccggttagc ccgagcaaag cagttattat caaaaccttt caggataaag
8701 gtgccaaagt gatttatggc gtgatcaacg ataaagaatg catggaaaaa attctgaaag
8761 agtacgagat cgacgttgtt attagcctgg tgggtggtgc acgtctgctg gatcagctga
8821 ccctgctgga agcaattaaa agcgttaaaa ccatcaaacg ttttctgccg agcgaatttg
8881 gccatgatgt tgatcgtacc gatccggttg aaccgggtct gaccatgtat aaagaaaaac
8941 gtctggtgcg tcgtgccgtt gaagaatatg gtattccgtt taccaatatc tgctgcaata
9001 gcattgcaag ctggccgtat tatgataatt gtcatccgag ccaggttccg cctccgatgg
9061 atcagtttca gatttatggt gatggtaaca ccaaagccta tttcattgat ggcaacgata
9121 tcggcaaatt taccatgaaa accatcgatg atattcgcac cctgaacaaa aatgttcatt
9181 ttcgtccgag cagcaactgc tacagcatta atgaactggc aagcctgtgg gagaaaaaaa
9241 tcggtcgtac actgcctcgt tttaccgtta ccgcagataa actgctggca catgcagcag
9301 aaaacattat tccggaaagc attgttagca gctttaccca cgatatcttt attaacggtt
9361 gccaggtgaa ctttagcatc gatgaacata gtgatgtgga aatcgataca ctgtatccgg
9421 atgaaaaatt tcgtagcctg gatgattgct atgaagattt tgttccgatg gtgcacgata
9481 aaattcatgc aggtaaaagc ggtgaaatca aaatcaaaga tggtaaaccg ctggttcaga
9541 ccggcaccat tgaagaaatt aacaaagaca ttaaaaccct ggtggaaacc cagccgaatg
9601 aagagatcaa aaaagatatg aaagcactgg ttgaagccgt tccgattagc gcaatgggtg
9661 gtgttaaaga aagcctggtt taactcgagt ctggtaaaga aaccgctgct gcgaaatttg
9721 aacgccagca catggactcg tctactagtc gcagcttaat taacctaaac tgctgccacc
9781 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
9841 ctagcgaaag gaggagtcga ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
9901 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
9961 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
10021 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
10081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
10141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
10201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
10261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
10321 atattaacgt ttacaatttc tggcggcacg atggcatgag attatcaaaa aggatcttca
10381 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
10441 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
10501 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
10561 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
10621 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
10681 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
10741 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
10801 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
10861 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
10921 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
10981 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
11041 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
11101 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
11161 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
11221 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
11281 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatgattga
11341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
11401 aaacaaatag gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
11461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
11521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
11581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
11641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
11701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
11761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
11821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
11881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
11941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
12001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
12061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
12121 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
12181 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
12241 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
12301 ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
12361 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
12421 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
12481 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
12541 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
12601 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
12661 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
12721 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
12781 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
12841 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
12901 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
12961 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
13021 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
13081 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
13141 ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
13201 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
13261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
13321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc
13381 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
13441 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc
13501 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca
13561 acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca
13621 accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg
13681 gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga
13741 tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac
13801 agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct
13861 tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc
13921 ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta
13981 atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg
14041 acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat
14101 ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca
14161 atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc
14221 tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc
14281 accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt
14341 actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg
14401 cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc
14461 ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa
14521 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
14581 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
14641 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
14701 tccggcgtag cctaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
14761 a
//
