质粒名称: | pUC118 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 50009 |
存储实验室: | Joachim Messing |
载体骨架基本信息 |
|
载体名称: | pUC18 |
载体出品公司: | Messing Lab |
载体抗性: | 氨苄霉素 |
载体修饰: | IG region of M13 from the HgiAI site (5465) to the DraI site (5941) inserted at the unique NdeI site (2499) of pUC. The orientation of the M13 IG region is such that the lac region that is packaged as ssDNA is the same as in the M13mp vectors. |
载体类型: | cloning vector |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
克隆方法: | 限制性内切酶 |
5' 测序引物 | M13-F20 |
3' 测序引物 | M13R |
文献引用方法 |
|
材料和方法部分: | pUC118 was a gift from Joachim Messing (爱迪基因 plasmid # 50009) |
参考文献部分: | Production of single-stranded plasmid DNA. Vieira J, Messing J. Methods Enzymol. 1987;153:3-11. PubMed 3323803 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUC118 3162 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION pUC cloning vector. ACCESSION . VERSION . KEYWORDS pUC118 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3162) AUTHORS Vieira J, Messing J TITLE Production of single-stranded plasmid DNA. JOURNAL Methods Enzymol. 1987;153:3-11. PUBMED 3323803 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pUC118| FEATURES Location/Qualifiers source 1..3162 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 217..543 /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRP VAAH" misc_feature 231..287 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(291..308) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(291..307) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(300..322) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 520..975 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(607..626) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 817..838 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(992..1011) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1111..1133 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1171..1189) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1257..1361 /gene="bla" /label=AmpR promoter CDS 1362..2222 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1580..1599) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2393..2981 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2882..2901 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3135..3152 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc 241 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc 301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 421 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg 481 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat 541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 721 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt 781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 841 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg 901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 961 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 1021 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 1081 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 1141 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 1201 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 1381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 1441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 1501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 1561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 1621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 1681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 1741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 1801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 1861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 1921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 1981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 2041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 2101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 2161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 2221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 2281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 2341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 2401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 2461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 2521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 2581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 2641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 2701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 2761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 2821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 2881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 2941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 3061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 3121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pCAG-luciferase
- 下一篇:p1585588