出品人: | Addgene |
---|---|
载体名称: | pMSCV-FLIP-puro-dsRed-GFP-miRNA |
质粒类型: | 逆病毒载体;RNAi载体;Cre/Lox重组系统 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 8376 bp |
5' 测序引物及序列: | pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3' |
3' 测序引物及序列: | -- |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | DH5α、Stbl3等 |
宿主细胞(系): | 常规哺乳动物细胞系 |
备注: |
推荐使用逆病毒包装载体:pUMVC、pCMV-VSV-G 。 |
产品目录号: | #32704 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
买家导航
pMSCV-FLIP-puro-dsRed-GFP-miRNA载体质粒基本信息
pMSCV-FLIP-puro-dsRed-GFP-miRNA质粒图谱载体图谱和pMSCV-FLIP-puro-dsRed-GFP-miRNA载体序列质粒序列多克隆位点信息
pMSCV-FLIP-puro-dsRed-GFP-miRNA质粒载体简介
pMSCV-FLIP-puro-dsRed-GFP-miRNA质粒序列载体序列
LOCUS pMSCV-FLIP-puro-dsRed-GFP-miRNA 8376 bp DNA SYN DEFINITION pMSCV-FLIP-puro-dsRed-GFP-miRNA ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8376 /organism="pMSCV-FLIP-puro-dsRed-GFP-miRNA" /mol_type="other DNA" misc_feature 655..1404 /label="psi_plus_pack" misc_feature 991..1398 /label="gag" misc_feature 1334..1356 /label="pLXSN_5_primer" misc_feature 1334..1356 /label="MSCV_primer" misc_feature 1372..1388 /label="pBABE_5_primer" misc_feature complement(1417..1450) /label="loxP" misc_feature 1417..1450 /label="loxP" CDS 1467..2852 /label="ORF frame 3" /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY ERAEARHHLFQ*" gene 1479..2054 /label="puro (variant)" /gene="puro (variant)" /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY ERAEARHHLFQ*" misc_feature complement(2355..2375) /label="dsRed1_N_primer" /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY ERAEARHHLFQ*" misc_feature 2767..2790 /label="dsRed1_C_primer" /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGALYKKQKIVAPVKQTLNFDL LKLAGDVESNPGPGPVATMDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEG TQTAKLQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMN FEDGGVVTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGV LKGEIHKALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQY ERAEARHHLFQ*" CDS complement(3152..3871) /label="ORF frame 3" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" gene complement(3155..3868) /label="EGFP (variant)" /gene="EGFP (variant)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature complement(3197..3218) /label="EGFP_C_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 3805..3826 /label="EGFP_N_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 3885..3918 /label="loxP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature complement(3965..3998) /label="loxP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 3965..3998 /label="loxP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKN GIKVNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" CDS 3978..4655 /label="ORF frame 3" /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP HRELSIK*" misc_feature 4034..4638 /label="WPRE" /translation="MCTIRSYPGLNDLQPSLSIIPDNQPLDYKICERLTGILNYVAPF TLCGYAALMPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPT GWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPW LLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASP HRELSIK*" CDS complement(4131..4634) /label="ORF frame 2" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" promoter complement(5369..5387) /label="M13_reverse_primer" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" misc_feature complement(5386..5408) /label="M13_pUC_rev_primer" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" promoter complement(5422..5451) /label="lac_promoter" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" gene complement(6534..7394) /label="Ampicillin" /gene="Ampicillin" /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS NSMIQRH*" CDS complement(6534..7394) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(7436..7464) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(7623..7645) /label="pGEX_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(7844..7975) /label="lacZ_a" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 7958..7980 /label="M13_pUC_fwd_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 8156..8175 /label="pBRrevBam_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT 301 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC 361 CCTCACTCGG CGCGCCAGTC CTCCGATAGA CTGCGTCGCC CGGGTACCCG TATTCCCAAT 421 AAAGCCTCTT GCTGTTTGCA TCCGAATCGT GGACTCGCTG ATCCTTGGGA GGGTCTCCTC 481 AGATTGATTG ACTGCCCACC TCGGGGGTCT TTCATTTGGA GGTTCCACCG AGATTTGGAG 541 ACCCCTGCCT AGGGACCACC GACCCCCCCG CCGGGAGGTA AGCTGGCCAG CGGTCGTTTC 601 GTGTCTGTCT CTGTCTTTGT GCGTGTTTGT GCCGGCATCT AATGTTTGCG CCTGCGTCTG 661 TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG GTGGAACTGA CGAGTTCTGA 721 ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTTG GGGGCCGTTT TTGTGGCCCG 781 ACCTGAGGAA GGGAGTCGAT GTGGAATCCG ACCCCGTCAG GATATGTGGT TCTGGTAGGA 841 GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT TGCTTTCGGT TTGGAACCGA 901 AGCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTTC TGTGTTGTCT CTGTCTGACT 961 GTGTTTCTGT ATTTGTCTGA AAATTAGGGC CAGACTGTTA CCACTCCCTT AAGTTTGACC 1021 TTAGGTCACT GGAAAGATGT CGAGCGGATC GCTCACAACC AGTCGGTAGA TGTCAAGAAG 1081 AGACGTTGGG TTACCTTCTG CTCTGCAGAA TGGCCAACCT TTAACGTCGG ATGGCCGCGA 1141 GACGGCACCT TTAACCGAGA CCTCATCACC CAGGTTAAGA TCAAGGTCTT TTCACCTGGC 1201 CCGCATGGAC ACCCAGACCA GGTCCCCTAC ATCGTGACCT GGGAAGCCTT GGCTTTTGAC 1261 CCCCCTCCCT GGGTCAAGCC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC 1321 GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA 1381 GCCCTCACTC CTTCTCTAGG CGCCGGAATT AGAtccataa cttcgtatag gataccttat 1441 acgaagttat ctcaggtacc GCCACCATGG TGGAGTACAA GCCCACGGTG CGCCTCGCCA 1501 CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG 1561 CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC 1621 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG 1681 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG 1741 GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC 1801 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC 1861 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG 1921 AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG 1981 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT 2041 GCATGACCCG CAAGCCCGGT GCCCTGTACA AGAAACAGAA AATTGTGGCA CCAGTGAAAC 2101 AGACTTTGAA TTTTGACCTT CTCAAGTTGG CGGGAGACGT CGAGTCCAAC CCTGGGCCCG 2161 GCCCGGTCGC CACCATGGAT AGCACTGAGA ACGTCATCAA GCCCTTCATG CGCTTCAAGG 2221 TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCA 2281 AGCCCTACGA GGGCACCCAG ACCGCCAAGC TGCAGGTGAC CAAGGGCGGC CCCCTGCCCT 2341 TCGCCTGGGA CATCCTGTCC CCCCAGTTCC AGTACGGCTC CAAGGTGTAC GTGAAGCACC 2401 CCGCCGACAT CCCCGACTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG 2461 TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG 2521 GCACCTTCAT CTACCACGTG AAGTTCATCG GCGTGAACTT CCCCTCCGAC GGCCCCGTAA 2581 TGCAGAAGAA GACTCTGGGC TGGGAGCCCT CCACCGAGCG CCTGTACCCC CGCGACGGCG 2641 TGCTGAAGGG CGAGATCCAC AAGGCGCTGA AGCTGAAGGG CGGCGGCCAC TACCTGGTGG 2701 AGTTCAAGTC AATCTACATG GCCAAGAAGC CCGTGAAGCT GCCCGGCTAC TACTACGTGG 2761 ACTCCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CGTGGTGGAG CAGTACGAGC 2821 GCGCCGAGGC CCGCCACCAC CTGTTCCAGT AGtctagaag ccataacttc gtatagtaca 2881 cattatacga aGTTATGCAT ACCTGGAGGA AAAAAAAAAG GGGAGAGTCA GGGGTTTAAA 2941 CTCGAGTGCG GCCAGATCTG GGCCATTTGT TCCATGTGAG TGCTAGTAAC AGGCCTTGTG 3001 TCCTGACAAC AGGCAGCATA ATGAGTCAGT CAGTGGCCAA AACTCATTAT GGACTGCCTG 3061 TTGTCAGCAT ACAGCCTTCA GCAAGCCTCC AGGATCCACT GGTCGACTCA CTACCTCCCT 3121 TTAAACGATC GACGGCCACG AAGTGCTTAG CTTACTTGTA CAGCTCGTCC ATGCCGAGAG 3181 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT 3241 CTTTGCTCAG GGCGGACTGG GTGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT 3301 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCTGCCG TCCTCGATGT 3361 TGTGGCGGGT CTTGAAGTTC ACCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA 3421 CCTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA 3481 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG 3541 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT 3601 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC 3661 ACTGCACGCC GTAGGTGAAG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG 3721 TGGTGCAGAT GAACTTCAGG GTCAGCTTGC CGTAGGTGGC ATCGCCCTCG CCCTCGCCGG 3781 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGATG GGCACCACCC 3841 CGGTGAACAG CTCCTCGCCC TTGCTCACCA TGGTGGCGac cggtataact tcgtataagg 3901 tatcctatac gaagttatcc attcaggctg tgctagcatc aatggcatgg cacaaagctt 3961 agccataact tcgtataatg tgtactatac gaagttatcc cgggttAAAC GACCTGCAGC 4021 CAAGCTTATC GATaattccc gataatcaac ctctggatta caaaatttgt gaaagattga 4081 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4141 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4201 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4261 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4321 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4381 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 4441 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4501 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 4561 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4621 ccgcctcccc gcatcgggaa ttATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA 4681 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT 4741 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA 4801 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA 4861 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT 4921 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAAATGACCC TGTGCCTTAT TTGAACTAAC 4981 CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG 5041 CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT 5101 GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG 5161 GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATGGGTAA CAGTTTCTTG 5221 AAGTTGGAGA ACAACATTCT GAGGGTAGGA GTCGAATATT AAGTAATCCT GACTCAATTA 5281 GCCACTGTTT TGAATCCACA TACTCCAATA CTCCTGAAAT AGTTCATTAT GGACAGCGCA 5341 GAAGAGCTGG GGAGAATTAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 5401 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT 5461 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG 5521 GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 5581 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 5641 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 5701 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 5761 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 5821 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 5881 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 5941 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 6001 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 6061 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 6121 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 6181 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 6241 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 6301 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 6361 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 6421 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 6481 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 6541 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 6601 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 6661 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 6721 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 6781 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 6841 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 6901 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 6961 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 7021 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 7081 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 7141 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 7201 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 7261 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 7321 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 7381 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 7501 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC 7561 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA 7621 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 7681 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC 7741 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC 7801 AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT 7861 TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT 7921 GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG 7981 ACGGCGCAAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA 8041 ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC 8101 CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA 8161 TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC 8221 GTCCGGCGTA GAGGCGATTA GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC 8281 TAGTTTTGAC TCAACAATAT CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAAAAT 8341 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAA //