出品人: | 爱迪基因 |
---|---|
载体名称: | pCLXSN |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 7819 bp |
5' 测序引物及序列: | pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3' |
3' 测序引物及序列: | -- |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | DH5α、HB101等 |
宿主细胞(系): | 常规哺乳动物细胞系 |
备注: |
推荐使用逆病毒包装载体:pUMVC、pCMV-VSV-G 。 |
产品目录号: | 12343 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
买家导航
pCLXSN载体质粒基本信息
pCLXSN质粒图谱载体图谱和pCLXSN载体序列质粒序列多克隆位点信息
pCLXSN质粒载体简介
pCLXSN质粒序列载体序列
LOCUS pCLXSN 7819 bp DNA SYN DEFINITION pCLXSN ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7819 /organism="pCLXSN" /mol_type="other DNA" promoter 8..582 /label="CMV_immearly_promoter" misc_feature 72..764 /label="5_LTR" misc_feature 87..373 /label="CAG_enhancer" misc_feature 539..559 /label="CMV_fwd_primer" misc_feature 834..1643 /label="psi_plus_pack" misc_feature 1230..1637 /label="gag" misc_feature 1573..1595 /label="pLXSN_5_primer" misc_feature 1573..1595 /label="MSCV_primer" misc_feature 1611..1627 /label="pBABE_5_primer" misc_feature complement(1685..1705) /label="pBABE_3_primer" misc_feature complement(1691..1906) /label="SV40_enhancer" promoter 1703..1971 /label="SV40_promoter" rep_origin 1870..1947 /label="SV40_origin" misc_feature 1932..1951 /label="SV40pro_F_primer" CDS 2079..2873 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 2082..2870 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 2933..3526 /label="3MoMuLV_LTR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 2975..3526 /label="5_LTR2" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature complement(3629..3651) /label="pGEX_3_primer" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 3872..3887 /label="SV40_int" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 3893..3940 /label="SV40_3_splice" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" terminator 4362..4481 /label="SV40_PA_terminator" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 4450..4469 /label="EBV_rev_primer" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" misc_feature 5388..5407 /label="SV40pro_F_primer" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" rep_origin complement(5681..6300) /label="pBR322_origin" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene complement(6455..7313) /label="Ampicillin" /gene="Ampicillin" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(6455..7021) /label="ORF frame 1" /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV IYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(7355..7383) /label="AmpR_promoter" /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV IYTTGSQATMDERNRQIAEIGASLIKHW*" misc_feature complement(7542..7564) /label="pGEX_3_primer" /translation="MTWLSSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGG PKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLA SRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVV IYTTGSQATMDERNRQIAEIGASLIKHW*" ORIGIN 1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttccatag taacgccaat 181 agggactttc cattgacgtc aatgggtgga ctatttacgg taaactgccc acttggcagt 241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 361 cgtattagtc atcgctatta ccatgtgatg cggttttggc agtacatcaa tgggcgtgga 421 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 481 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 541 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcaGagctcA ATAAAAGAGC 601 CCACAACCCC TCACTCGGCG CGCCAGTCTT CCGATAGACT GCGTCGCCCG GGTACCCGTA 661 TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG TCTCGCTGTT CCTTGGGAGG 721 GTCTCCTCTG AGTGATTGAC TACCCACGAC GGGGGTCTTT CATTTGGGGG CTCGTCCGGG 781 ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC TGGCCAGCAA 841 CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGTTTGA TGTTATGCGC CTGCGTCTGT 901 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 961 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 1021 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 1081 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 1141 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 1201 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1261 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1321 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1381 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1441 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1501 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1561 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1621 CCCTCACTCC TTCTCTAGGC GCCGGAATTG CGGCCGCAAT TCGTTAACTC GAGGATCCGG 1681 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 1741 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 1801 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 1861 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 1921 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 1981 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGGGCT GCAGGTCGAG 2041 GCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 2101 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 2161 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 2221 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 2281 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 2341 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 2401 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 2461 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 2521 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 2581 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 2641 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 2701 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 2761 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 2821 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 2881 TCTGGGGTTC GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG 2941 ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA 3001 ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT 3061 ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG 3121 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT 3181 CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC 3241 ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA 3301 CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA 3361 GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG 3421 TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG 3481 GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATTTGGG GGCTCGTCCG 3541 GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC CGGGAGGTAA GCTGGCTGCC 3601 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 3661 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 3721 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACct 3781 agctaggtag ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 3841 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 3901 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg 3961 aatgggagca gtggtccaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc 4021 ctctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag 4081 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt 4141 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact 4201 gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta gagatcataa 4261 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 4321 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 4381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 4441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctga tcccgccatg 4501 gtatcaacgc catatttcta tttacagtag ggacctcttc gttgtgtagg taccgctgta 4561 ttcctaggga aatagtagag gcaccttgaa ctgtctgcat cagccatata gcccccgctg 4621 ttcgacttac aaacacaggc acagtactga caaacccata cacctcctct gaaataccca 4681 tagttgctag ggctgtctcc gaactcatta caccctccaa agtcagagct gtaatttcgc 4741 catcaagggc agcgagggct tctccagata aaatagcttc tgccgagagt cccgtaaggg 4801 tagacacttc agctaatccc tcgatgaggt ctactagaat agtcagtgcg gctcccattt 4861 tgaaaattca cttacttgat cagcttcaga agatggcgga gggcctccaa cacagtaatt 4921 ttcctcccga ctcttaaaat agaaaatgtc aagtcagtta agcaggaagt ggactaactg 4981 acgcagctgg ccgtgcgaca tcctctttta attagttgct aggcaacgcc ctccagaggg 5041 cgtgtggttt tgcaagagga agcaaaagcc tctccaccca ggcctagaat gtttccaccc 5101 aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg ggacccctgg 5161 cccgcttact ctggagaaaa aaaacattgt agaggcttcc agaggcaact tgtcaaaaca 5221 ggactgcttc tatttctgtc acactgtctg gccctgtcac aaggtccagc acctccatac 5281 cccctttaat aagcagtttg ggaacgggtg cgggtcttac tccgcccatc cgcccctaac 5341 tccgcccagt tccgcccatt ctccgcccca tgctgactaa ttttttttat ttatgcagag 5401 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggC 5461 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 5521 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 5581 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 5641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 5701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 5761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 5821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 5881 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 5941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6121 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 6241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg 6781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaACTC AACCAAGTCA 7021 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT 7081 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA 7141 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 7201 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG 7261 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC 7321 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 7381 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 7441 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 7501 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC 7561 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC 7621 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT 7681 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC 7741 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG 7801 GCCTCTTCGC TATTACGCC //