| 质粒名称: | pEGFP Tensin3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 105299 |
| 存储实验室: | David Critchley, Kenneth Yamada |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tensin3,TNS3,NM_022748 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4300 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | EGFP（主干上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoR1（销毁后未知） |
| 3' 克隆位点: | Sal1（销毁后未知） |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP Tensin3 was a gift from David Critchley & Kenneth Yamada (Addgene plasmid # 105299 ; http://n2t.net/addgene:105299 ; RRID:Addgene_105299) |
| 参考文献部分: | Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1. Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM, Critchley DR. J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460. 10.1002/jcb.22460 PubMed 20069572 |
买家导航
pEGFPTensin3载体基本信息
pEGFPTensin3载体图谱质粒图谱和多克隆位点信息
pEGFPTensin3载体简介
pEGFPTensin3载体序列
LOCUS pEGFP Tensin3 9055 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION To study localization and function of Tensin3.
ACCESSION .
VERSION .
KEYWORDS pEGFP Tensin3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9055)
AUTHORS Clark K, Howe JD, Pullar CE, Green JA, Artym VV, Yamada KM,
Critchley DR
TITLE Tensin 2 modulates cell contractility in 3D collagen gels through
the RhoGAP DLC1.
JOURNAL J Cell Biochem. 2010 Mar 1;109(4):808-17. doi: 10.1002/jcb.22460.
PUBMED 20069572
REFERENCE 2 (bases 1 to 9055)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9055
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 6..594
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 495..514
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 758..1061
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1062..1265
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1216..1236
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 1304..1313
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1310..2026
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1355..1376)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1616..1635)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1963..1984
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 6551..6672
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6588..6607)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6642..6661
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(6679..7134)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6816..6837)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7028..7047
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 7161..7265
/gene="bla"
/label=AmpR promoter
promoter 7267..7624
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(7290..7310)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 7475..7610
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7537..7556
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7659..8453
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7713..7732)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8323..8342
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(8641..8660)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 8685..8732
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
61 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
601 agcaacgcgg cctttttacg gttcctggcc ttttgctcac atgttctttc ctgcgttatc
661 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
721 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
781 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
841 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
901 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
961 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
1021 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
1081 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
1141 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
1201 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
1261 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtcgccacca tggtgagcaa
1321 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
1381 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
1441 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
1501 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
1561 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
1621 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
1681 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
1741 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
1801 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
1861 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
1921 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
1981 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
2041 tcgagctcaa gcttcgaatt ccatggagga gggccatggg ctggacctca cttacatcac
2101 ggagcgcatc atcgctgtgt ccttccctgc cggctgctct gaggagtcct acctgcacaa
2161 cctacaggag gtcacgcgca tgctcaagtc caagcacggg gacaactacc tggtattaaa
2221 cctttcagaa aagagatatg accttacgaa gcttaaccca aagatcatgg atgtgggctg
2281 gccagagctc cacgcaccgc ccctggataa gatgtgtacc atatgcaagg cgcaggagtc
2341 ctggctgaac agcaacctcc agcatgtggt cgtcattcac tgcaggggcg ggaaaggacg
2401 cataggagtg gtcatatcat cctacatgca tttcaccaac gtctcagcca gcgccgacca
2461 ggcccttgac aggtttgcaa tgaagaagtt ttatgatgac aaagtttcag ctttaatgca
2521 gccttcccaa aaacggtatg ttcagttcct cagtgggctc ctgtccggat cggtgaaaat
2581 gaatgcctct cccctgttcc tgcattttgt catcctccac ggcaccccca acttcgacac
2641 aggtggagtg tgccggccct ttctgaagct ctaccaagcc atgcagcctg tgtacacctc
2701 cgggatctac aacgttggcc cagaaaaccc cagcaggatc tgcatcgtca tcgagccggc
2761 ccagcttctg aagggagatg tcatggtgaa atgctaccac aagaaatacc gctcggccac
2821 ccgtgacgtc attttccgcc tgcagtttca cactggggct gtgcagggct acgggctggt
2881 gtttgggaag gaggatctgg acaatgccag caaagatgac cgttttcctg actatgggaa
2941 ggttgaatta gtcttctctg ccacgcctga gaagattcaa gggtccgaac acttgtacaa
3001 cgaccacggt gtgattgtgg actacaacac aacagaccca ctgatacgct gggactcgta
3061 cgagaacctc agtgcagatg gagaagtgct acacacgcag ggccctgtcg atggcagcct
3121 ttacgcgaag gtgaggaaga aaagctcctc ggatcctggc atcccaggtg gcccccaggc
3181 aatcccggcc accaacagcc cagaccacag tgaccacacc ttgtctgtca gcagtgactc
3241 cggccactct acagcctctg ccaggacgga taagacggaa gagcgcctgg ccccaggaac
3301 caggaggggc ctgagtgccc aggagaaggc tgagttggac cagctgctca gtggctttgg
3361 cctggaagat cctggaagct ccctcaagga aatgactgat gctcgaagca agtacagtgg
3421 gacccgccac gtggtgccag cccaggttca cgtgaatgga gacgctgctc tgaaggatcg
3481 ggagacagac attctggatg acgagatgcc ccaccacgac ctgcacagtg tggacagcct
3541 tgggaccctg tcctcctcgg aagggcctca gtcggcccac ctgggtccct tcacctgcca
3601 caagagcagc cagaactcac tcctatctga cggttttggc agcaacgttg gtgaagatcc
3661 gcagggcacc ctcgttccgg acctgggcct tggcatggac ggcccctatg agcgggagcg
3721 gacttttggg agtcgagagc ccaagcagcc ccagcccctg ctgagaaagc cctcagtgtc
3781 cgcccagatg caggcctatg ggcagagcag ctactccaca cagacctggg tgcgccagca
3841 gcagatggtt gtagctcacc agtatagctt cgccccagat ggggaggccc ggctggtgag
3901 ccgctgccct gcagacaatc ctggcctcgt ccaggcccag cccagagtgc cactcacccc
3961 cacccgaggg accagcagta gggtggctgt ccagaggggt gtaggcagtg ggccacatcc
4021 ccctgacaca cagcagccct ctcccagcaa agcgttcaaa cccaggtttc caggagacca
4081 ggttgtgaat ggagccggcc cagagctgag cacaggcccc tccccaggct cgcccaccct
4141 ggacatcgac cagtccatcg agcagctcaa caggctgatc ctggagctgg atcccacctt
4201 cgagcccatc cctacccaca tgaacgccct cggtagccag gccaatggct ctgtgtctcc
4261 agacagcgtg ggaggcgggc tccgggcaag cagcaggctg cctgacacag gagagggccc
4321 cagcagggcc accgggcggc aaggctcctc tgctgaacag cccctgggcg ggagactcag
4381 gaagctgagc ctggggcagt acgacaacga tgctgggggg cagctgccct tctccaaatg
4441 tgcatgggga aaggctggtg tggactatgc cccaaacctg ccgccattcc cctcaccagc
4501 ggacgtcaaa gagacgatga cccctggcta tccccaggac ctcgatatta tcgatggcag
4561 aattttaagt agcaaggagt ccatgtgttc aactccagca tttcctgtgt ctccagagac
4621 accgtatgtg aaaacagcgc tgcgccatcc tccgttcagc ccacctgagc ccccgctgag
4681 cagcccagcc agtcagcaca aaggaggacg tgaaccacga agctgccctg agacgctcac
4741 tcacgctgtg gggatgtcag agagccccat cggacccaaa tccacgatgc tccgggctga
4801 tgcgtcctcg acgccctcct ttcagcaggc ttttgcttct tcctgcacca tttccagcaa
4861 cggccctggg cagaggagag agagctcctc ttctgcagaa cgccagtggg tggagagcag
4921 ccccaagccc atggtttccc tgctggggag cggccggccc accggaagtc ccctcagcgc
4981 tgagttctcc ggtaccagga aggactcccc agtgctgtcc tgcttcccgc cgtcagagct
5041 ccaggctcct ttccacagcc atgagctgtc cctagcagag ccaccggact ccctggcgcc
5101 tcccagcagc caggccttcc tgggcttcgg caccgcccca gtgggaagtg gccttccgcc
5161 cgaggaggac ctgggggcct tgctggccaa ttctcatgga gcgtcaccga cccccagcat
5221 cccgctgaca gcgacagggg ctgccgacaa tggcttcctg tcccacaact ttctcacggt
5281 ggcgcctgga cacagcagcc accacagtcc aggcctgcag ggccagggtg tgaccctgcc
5341 cgggcagcca cccctccctg agaagaagcg ggcctcggag ggggatcgtt ctttgggctc
5401 agtctctccc tcctccagtg gcttctccag cccgcacagc gggagcacca tcagtatccc
5461 cttcccaaat gtccttcccg acttttccaa ggcttcagaa gcggcctcac ctctgccaga
5521 tagtccaggt gataaacttg tgatcgtgaa atttgttcaa gacacttcca agttctggta
5581 caaggcggat atttcaagag aacaagccat cgccatgttg aaggacaagg agccgggctc
5641 attcattgtt cgagacagcc attccttccg aggggcctat ggcctggcca tgaaggtggc
5701 cacgccccca ccttcagtcc tgcagctgaa caagaaagct ggagatttgg ccaatgaact
5761 cgtccggcac tttttgatcg agtgtacccc gaagggagtg cggttgaaag ggtgctcgaa
5821 tgaaccatat ttcgggagcc tgacggcctt ggtgtgccag cattccatca cgcccttggc
5881 cttgccgtac aagctgctta tcccagagag agatccattg gaggaaatag cagaaagttc
5941 tccccagacg gcagccaatt cagcagctga gctgttgaag cagggggcag cctgcaatgt
6001 gtggtacttg aactctgtgg agatggagtc cctcaccggc caccaggcga tccagaaggc
6061 cctgagcatc accctggtcc aggagcctcc acctgtgtcc acagttgtgc acttcaaggt
6121 gtcagcccag ggcatcaccc tgacagacaa tcagaggaag ctcttcttcc ggaggcatta
6181 ccccgtgaac agtgtgattt tctgtgcctt ggacccacaa gacaggaagt ggatcaaaga
6241 tggcccttcc tcaaaagtct ttggatttgt ggcccggaag cagggcagtg ccacggataa
6301 tgtgtgccac ctgtttgcag agcatgaccc tgagcagcct gccagtgcca ttgtcaactt
6361 cgtatcaaag gtcatgattg gttcaccaaa gaaggtctga gtcgacggta ccgcgggccc
6421 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
6481 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
6541 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
6601 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
6661 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
6721 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
6781 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
6841 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
6901 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
6961 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
7021 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
7081 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
7141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
7201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
7261 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
7321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
7381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
7441 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
7501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
7561 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
7621 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
7681 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
7741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
7801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
7861 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
7921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
7981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
8041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
8101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
8161 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
8221 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
8281 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
8341 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
8401 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
8461 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
8521 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
8581 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
8641 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
8701 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
8761 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
8821 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
8881 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
8941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
9001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aagga
//
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