| 质粒名称: | FUW-tetO-hOKMS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 51543 |
| 存储实验室: | Tarjei Mikkelsen |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 13399 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | hOKMS |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4958 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | tetO |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | agagctcgtttagtgaaccg |
| 3’测序引物 | gttgcgtcagcaaacacagt |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-hOKMS was a gift from Tarjei Mikkelsen (Addgene plasmid # 51543 ; http://n2t.net/addgene:51543 ; RRID:Addgene_51543) |
| 参考文献部分: | Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of Induced Pluripotency. Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ, Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner A, Lander ES, Mikkelsen TS. Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016. 10.1016/j.cell.2015.06.016 PubMed 26186193 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW-tetO-hOKMS 13356 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2.
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-hOKMS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13356)
AUTHORS Cacchiarelli D, Trapnell C, Ziller MJ, Soumillon M, Cesana M, Karnik
R, Donaghey J, Smith ZD, Ratanasirintrawoot S, Zhang X, Ho Sui SJ,
Wu Z, Akopian V, Gifford CA, Doench J, Rinn JL, Daley GQ, Meissner
A, Lander ES, Mikkelsen TS
TITLE Integrative Analyses of Human Reprogramming Reveal Dynamic Nature of
Induced Pluripotency.
JOURNAL Cell. 2015 Jul 16;162(2):412-24. doi: 10.1016/j.cell.2015.06.016.
PUBMED 26186193
REFERENCE 2 (bases 1 to 13356)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13356
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 7..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 388..586
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 604..784
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 831..956
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1449..1682
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1867..1911
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2209..2326
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2383..2401
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2425..2443
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2467..2485
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2509..2527
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2551..2569
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2593..2611
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 2644..2682
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2679..2703
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3915..3968
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5388..5444
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 5445..6761
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
CDS 6771..6830
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/label=E2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="QCTNYALLKLAGDVESNPGP"
misc_feature 7817..8405
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7870..7890)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8288..8299)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8408..8424)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8409..8425)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8930..9110
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9136..9153)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9142..9366
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9412..9840
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9499..9518)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9709..9730
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9849..9869)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9854..10183
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10034..10169
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10096..10115
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10231..10278
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10297..10671
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10801..10922
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10838..10857)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10892..10911
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10971..10987)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10971..10987)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10984..11006)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10995..11011
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11019..11049)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11064..11085
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11202..11219)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11373..11961)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11453..11472)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12132..12992)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12755..12774
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12993..13097)
/gene="bla"
/label=AmpR promoter
primer_bind complement(13172..13191)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
2641 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
2701 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
2761 agcctccgcg gccccgaatt cgttgaccga atcaccgacc tctctcccca gggggatcca
2821 ccggtatggc gggacacctg gcttcggatt tcgccttctc gccccctcca ggtggtggag
2881 gtgatgggcc aggggggccg gagccgggct gggttgatcc tcggacctgg ctaagcttcc
2941 aaggccctcc tggagggcca ggaatcgggc cgggggttgg gccaggctct gaggtgtggg
3001 ggattccccc atgccccccg ccgtatgagt tctgtggggg gatggcgtac tgtgggcccc
3061 aggttggagt ggggctagtg ccccaaggcg gcttggagac ctctcagcct gagggtgaag
3121 caggagtcgg ggtggagagc aactccgatg gggcctcccc ggagccctgc accgtcaccc
3181 ctggtgccgt gaagctggag aaggagaagc tggagcaaaa cccggaggag tcccaggaca
3241 tcaaagctct gcagaaagaa ctcgagcaat ttgccaagct cctgaagcag aagaggatca
3301 ccctgggata tacacaggcc gatgtggggc tcaccctggg ggttctattt gggaaggtat
3361 tcagccaaac gaccatctgc cgctttgagg ctctgcagct tagcttcaag aacatgtgta
3421 agctgcggcc cttgctgcag aagtgggtgg aggaagctga caacaatgaa aatcttcagg
3481 agatatgcaa agcagaaacc ctcgtgcagg cccgaaagag aaagcgaacc agtatcgaga
3541 accgagtgag aggcaacctg gagaatttgt tcctgcagtg cccgaaaccc acactgcagc
3601 agatcagcca catcgcccag cagcttgggc tcgagaagga tgtggtccga gtgtggttct
3661 gtaaccggcg ccagaagggc aagcgatcaa gcagcgacta tgcacaacga gaggattttg
3721 aggctgctgg gtctcctttc tcagggggac cagtgtcctt tcctctggcc ccagggcccc
3781 attttggtac cccaggctat gggagccctc acttcactgc actgtactcc tcggtccctt
3841 tccctgaggg ggaagccttt ccccctgtct ctgtcaccac tctgggctct cccatgcatt
3901 caaacggatc tggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
3961 ccggccccat ggctgtcagc gacgcgctgc tcccatcttt ctccacgttc gcgtctggcc
4021 cggcgggaag ggagaagaca ctgcgtcaag caggtgcccc gaataaccgc tggcgggagg
4081 agctctccca catgaagcga cttcccccag tgcttcccgg ccgcccctat gacctggcgg
4141 cggcgaccgt ggccacagac ctggagagcg gcggagccgg tgcggcttgc ggcggtagca
4201 acctggcgcc cctacctcgg agagagaccg aggagttcaa cgatctcctg gacctggact
4261 ttattctctc caattcgctg acccatcctc cggagtcagt ggccgccacc gtgtcctcgt
4321 cagcgtcagc ctcctcttcg tcgtcgccgt cgagcagcgg ccctgccagc gcgccctcca
4381 cctgcagctt cacctatccg atccgggccg ggaacgaccc gggcgtggcg ccgggcggca
4441 cgggcggagg cctcctctat ggcagggagt ccgctccccc tccgacggct cccttcaacc
4501 tggcggacat caacgacgtg agcccctcgg gcggcttcgt ggccgagctc ctgcggccag
4561 aattggaccc ggtgtacatt ccgccgcagc agccgcagcc gccaggtggc gggctgatgg
4621 gcaagttcgt gctgaaggcg tcgctgagcg cccctggcag cgagtacggc agcccgtcgg
4681 tcatcagcgt cagcaaaggc agccctgacg gcagccaccc ggtggtggtg gcgccctaca
4741 acggcgggcc gccgcgcacg tgccccaaga tcaagcagga ggcggtctct tcgtgcaccc
4801 acttgggcgc tggaccccct ctcagcaatg gccaccggcc ggctgcacac gacttccccc
4861 tggggcggca gctccccagc aggactaccc cgaccctggg tcttgaggaa gtgctgagca
4921 gcagggactg tcaccctgcc ctgccgcttc ctcccggctt ccatccccac ccggggccca
4981 attacccatc cttcctgccc gatcagatgc agccgcaagt cccgccgctc cattaccaag
5041 agctcatgcc acccggttcc tgcatgccag aggagcccaa gccaaagagg ggaagacgat
5101 cgtggccccg gaaaaggacc gccacccaca cttgtgatta cgcgggctgc ggcaaaacct
5161 acacaaagag ttcccatctc aaggcacacc tgcgaaccca cacaggtgag aaaccttacc
5221 actgtgactg ggacggctgt ggatggaaat tcgcccgctc agatgaactg accaggcact
5281 accgtaaaca cacggggcac cgcccgttcc agtgccaaaa atgcgaccga gcattttcca
5341 ggtcggacca cctcgcctta cacatgaaga ggcattttgg atctggagca acaaacttct
5401 cactactcaa acaagcaggt gacgtggagg agaatcccgg ccctatgccc ctcaacgtta
5461 gcttcaccaa caggaactat gacctcgact acgactcggt gcagccgtat ttctactgcg
5521 acgaggagga gaacttctac cagcagcagc agcagagcga gctgcagccc ccggcgccca
5581 gcgaggatat ctggaagaaa ttcgagctgc tgcccacccc gcccctgtcc cctagccgcc
5641 gctccgggct ctgctcgccc tcctacgttg cggtcacacc cttctccctt cggggagaca
5701 acgacggcgg tggcgggagc ttctccacgg ccgaccagct ggagatggtg accgagctgc
5761 tgggaggaga catggtgaac cagagtttca tctgcgaccc ggacgacgag accttcatca
5821 aaaacatcat catccaggac tgtatgtgga gcggcttctc ggccgccgcc aagctcgtct
5881 cagagaagct ggcctcctac caggctgcgc gcaaagacag cggcagcccg aaccccgccc
5941 gcggccacag cgtctgctcc acctccagct tgtacctgca ggatctgagc gccgccgcct
6001 cagagtgcat cgacccctcg gtggtcttcc cctaccctct caacgacagc agctcgccca
6061 agtcctgcgc ctcgcaagac tccagcgcct tctctccgtc ctcggattct ctgctctcct
6121 cgacggagtc ctccccgcag ggcagccccg agcccctggt gctccatgag gagacaccgc
6181 ccaccaccag cagcgactct gaggaggaac aagaagatga ggaagaaatc gatgttgttt
6241 ctgtggaaaa gaggcaggct cctggcaaaa ggtcagagtc tggatcacct tctgctggag
6301 gccacagcaa acctcctcac agcccactgg tcctcaagag gtgccacgtc tccacacatc
6361 agcacaacta cgcagcgcct ccctccactc ggaaggacta tcctgctgcc aagagggtca
6421 agttggacag tgtcagagtc ctgagacaga tcagcaacaa ccgaaaatgc accagcccca
6481 ggtcctcgga caccgaggag aatgtcaaga ggcgaacaca caacgtcttg gagcgccaga
6541 ggaggaacga gctaaaacgg agcttttttg ccctgcgtga ccagatcccg gagttggaaa
6601 acaatgaaaa ggcccccaag gtagttatcc ttaaaaaagc cacagcatac atcctgtccg
6661 tccaagcaga ggagcaaaag ctcatttctg aagaggactt gttgcggaaa cgacgagaac
6721 agttgaaaca caaacttgaa cagctacgga actcttgtgc gggatctgga caatgtacta
6781 actacgcttt gttgaaactc gctggcgatg ttgaaagtaa ccccggtccc atgtacaaca
6841 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
6901 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
6961 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
7021 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
7081 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
7141 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
7201 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
7261 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
7321 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
7381 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
7441 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
7501 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
7561 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
7621 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
7681 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
7741 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagtcgac aagaattcga
7801 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
7861 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
7921 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
7981 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
8041 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
8101 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
8161 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
8221 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
8281 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
8341 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
8401 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
8461 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
8521 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
8581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
8641 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
8701 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
8761 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
8821 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
8881 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
8941 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
9001 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
9061 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
9121 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
9181 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
9241 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
9301 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
9361 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
9421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
9481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
9541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
9601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
9661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
9721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
9781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
9841 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
9901 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
9961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
10021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
10081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
10141 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
10201 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
10261 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
10321 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
10381 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
10441 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
10501 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
10561 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
10621 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
10681 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
10741 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
10801 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
10861 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
10921 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
10981 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
11041 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
11101 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
11161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
11221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
11281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
11341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
11401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
11461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
11521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
11581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
11641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
11701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
11761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
11821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
11881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
11941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
12001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
12061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
12121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
12181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
12241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
12301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
12361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
12421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
12481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
12541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
12601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
12661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
12721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
12781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
12841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
12901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
12961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
13021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
13081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
13141 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
13201 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
13261 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
13321 gttttgcgct gcttcgcgat gtacgggcca gatata
//
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