出品公司: | Addgene |
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载体名称: | PX387 或 PX387 Mycoplasma mobile Cas9 |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10904 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter);HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞(系): | 昆虫细胞 |
备注: | -- |
产品目录号: | #68324 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
PX387载体质粒基本信息
PX387质粒图谱载体图谱和PX387载体序列质粒序列多克隆位点信息
PX387质粒载体简介
PX387质粒序列载体序列
LOCUS PX387 Mycoplasma mobile Cas9 10904 bp DNA SYN DEFINITION PX387 Mycoplasma mobile Cas9 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10904 /organism="PX387 Mycoplasma mobile Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5595 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMYFYKNKENKLNKKVVLGLDLGI ASVGWCLTDISQKEDNKFPIILHGVRLFETVDDSDDKLLNETRRKKRGQRRRNRRLFT RKRDFIKYLIDNNIIELEFDKNPKILVRNFIEKYINPFSKNLELKYKSVTNLPIGFHN LRKAAINEKYKLDKSELIVLLYFYLSLRGAFFDNPEDTKSKEMNKNEIEIFDKNESIK NAEFPIDKIIEFYKISGKIRSTINLKFGHQDYLKEIKQVFEKQNIDFMNYEKFAMEEK SFFSRIRNYSEGPGNEKSFSKYGLYANENGNPELIINEKGQKIYTKIFKTLWESKIGK CSYDKKLYRAPKNSFSAKVFDITNKLTDWKHKNEYISERLKRKILLSRFLNKDSKSAV EKILKEENIKFENLSEIAYNKDDNKINLPIINAYHSLTTIFKKHLINFENYLISNEND LSKLMSFYKQQSEKLFVPNEKGSYEINQNNNVLHIFDAISNILNKFSTIQDRIRILEG YFEFSNLKKDVKSSEIYSEIAKLREFSGTSSLSFGAYYKFIPNLISEGSKNYSTISYE EKALQNQKNNFSHSNLFEKTWVEDLIASPTVKRSLRQTMNLLKEIFKYSEKNNLEIEK IVVEVTRSSNNKHERKKIEGINKYRKEKYEELKKVYDLPNENTTLLKKLWLLRQQQGY DAYSLRKIEANDVINKPWNYDIDHIVPRSISFDDSFSNLVIVNKLDNAKKSNDLSAKQ FIEKIYGIEKLKEAKENWGNWYLRNANGKAFNDKGKFIKLYTIDNLDEFDNSDFINRN LSDTSYITNALVNHLTFSNSKYKYSVVSVNGKQTSNLRNQIAFVGIKNNKETEREWKR PEGFKSINSNDFLIREEGKNDVKDDVLIKDRSFNGHHAEDAYFITIISQYFRSFKRIE RLNVNYRKETRELDDLEKNNIKFKEKASFDNFLLINALDELNEKLNQMRFSRMVITKK NTQLFNETLYSGKYDKGKNTIKKVEKLNLLDNRTDKIKKIEEFFDEDKLKENELTKLH IFNHDKNLYETLKIIWNEVKIEIKNKNLNEKNYFKYFVNKKLQEGKISFNEWVPILDN DFKIIRKIRYIKFSSEEKETDEIIFSQSNFLKIDQRQNFSFHNTLYWVQIWVYKNQKD QYCFISIDARNSKFEKDEIKINYEKLKTQKEKLQIINEEPILKINKGDLFENEEKELF YIVGRDEKPQKLEIKYILGKKIKDQKQIQKPVKKYFPNWKKVNLTYMGEIFKK*" CDS complement(2809..3273) /label="ORF frame 3" /translation="MQDVIVLVDFVGALLIGHKQFLALLLVERHQLGQVIFVTDQIVL EINQMLLEDCGQRVVGVNDWQVNLIIVLVVCDLGQIFELNVFLLQDFLHGTLRIFVQK PRQQNFSLQALGDVFVFVLPISQLICDVKHFSGEAVLWGSIQFLVVAALPDF*" CDS complement(4405..4923) /label="ORF frame 3" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" misc_feature complement(5628..5645) /label="Sp6_primer" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" misc_feature 5650..5674 /label="attB2" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" terminator 5686..6152 /label="hGH_PA_terminator" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" terminator 6158..6193 /label="T7_terminator" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" rep_origin 6310..6616 /label="f1_origin" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" misc_feature complement(6730..6750) /label="pBABE_3_primer" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" misc_feature complement(6736..6951) /label="SV40_enhancer" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" promoter 6748..7016 /label="SV40_promoter" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" rep_origin 6915..6992 /label="SV40_origin" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" misc_feature 6977..6996 /label="SV40pro_F_primer" /translation="MVEDVQLGQLIFLQFILVEELLDFLNLIGSIVQQVQFFDFLDSV LAFVILSAVQCLIEQLGVFLSDDHPRKSHLIQLLVQFIQSINQQKVVEGGLFFELDVI LLQIVQLSGFFPVIHVQALNPLKTPEVLRDDSDEVGILCMVAVEAPILDQDIVFHVIL SFLSNQKVIAVD*" CDS 7131..7925 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 7134..7922 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7440..7976) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8102..8221 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 8190..8209 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8412..8447 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8470..8487) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 8477..8554 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(8584..9201) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 9181..9214 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9982..10842) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9982..10842) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag 1861 aaaaagcgca aggtcgaagc gtccatgtac ttctataaaa ataaggaaaa caaactgaac 1921 aaaaaggtgg tgctggggct ggatctggga atcgcttctg tcgggtggtg cctgaccgac 1981 atctcccaga aagaggataa caagttccct atcattctgc acggagtgcg cctgtttgag 2041 actgtcgacg attctgacga taagctgctg aatgaaaccc ggagaaagaa acgaggccag 2101 aggcgccgaa accggagact gttcacaagg aagcgcgact ttatcaagta cctgatcgat 2161 aacaatatca ttgagctgga gttcgacaaa aaccctaaga tcctggtgcg caacttcatc 2221 gagaagtaca tcaacccatt ttcaaagaat ctggaactga agtacaaaag cgtcactaac 2281 ctgccaatcg ggtttcacaa tctgcgcaaa gccgctatta acgagaagta caaactggat 2341 aagagtgaac tgatcgtgct gctgtacttc tatctgtcac tgcggggagc attctttgac 2401 aaccctgagg atacaaagtc aaaggaaatg aataagaacg agatcgaaat tttcgacaag 2461 aacgagtcca tcaaaaatgc cgagttccca atcgataaga tcatcgagtt ttacaagatt 2521 agcgggaaga tccggtccac tattaacctg aagtttggac atcaggacta cctgaaagag 2581 atcaagcagg tgttcgaaaa gcagaacatc gatttcatga actacgagaa gttcgctatg 2641 gaggaaaaat cattctttag ccgaatccgg aattacagcg aaggccccgg gaacgagaaa 2701 agtttttcaa agtacgggct gtatgcaaat gaaaacggaa atcctgagct gatcattaat 2761 gaaaagggcc agaagatcta tacaaagatt ttcaagactc tgtgggagtc aaaaatcggg 2821 aagtgcagct acgacaagaa actgtataga gccccaaaga acagcttctc cgctaaagtg 2881 tttgacatca caaataagct gactgattgg aagcacaaaa acgaatacat ctccgagcgc 2941 ctgaagcgaa aaattctgct gtctaggttt ctgaacaaag attctaagag tgccgtggag 3001 aaaatcctga aggaggaaaa cattaagttc gaaaatctgt ccgagatcgc atacaacaag 3061 gacgataata agattaacct gccaatcatt aacgcctacc actctctgac cacaatcttc 3121 aagaagcatc tgattaattt cgagaactat ctgatcagta acgaaaatga cctgtccaag 3181 ctgatgtctt tctacaagca gcagagcgag aaactgtttg tgcccaatga gaagggctcc 3241 tacgaaatca accagaacaa taacgtcctg catattttcg acgccatctc taacattctg 3301 aacaagttta gtactatcca ggatagaatc aggattctgg aagggtattt cgagttttct 3361 aacctgaaga aagacgtgaa aagctccgaa atctacagtg agattgctaa gctgcgggag 3421 ttttccggca cctctagtct gtctttcggg gcatactata agttcatccc caatctgatt 3481 tccgagggct ctaagaacta cagtacaatc tcatatgagg aaaaggccct gcagaatcag 3541 aaaaataact tctcacacag caacctgttt gagaaaactt gggtggagga cctgatcgct 3601 agtcctaccg tcaagcggtc actgagacag acaatgaatc tgctgaaaga gatcttcaag 3661 tacagcgaga agaacaacct ggagatcgaa aagattgtgg tcgaggtgac caggtcaagc 3721 aataacaaac atgagcgcaa gaaaatcgaa ggaattaaca agtaccgcaa ggagaaatat 3781 gaggaactga agaaagtgta tgacctgccc aacgaaaata ctaccctgct gaagaaactg 3841 tggctgctgc gacagcagca gggctacgac gcatattccc tgcggaaaat cgaggccaat 3901 gatgtgatta acaagccatg gaattacgac atcgatcaca ttgtcccccg gtccatctct 3961 ttcgacgata gtttttcaaa cctggtcatc gtcaacaagc tggacaatgc taagaaaagt 4021 aacgatctgt cagcaaagca gttcatcgag aaaatctacg gcatcgagaa gctgaaagaa 4081 gccaaggaga attgggggaa ctggtatctg cgcaacgcta atggaaaagc attcaacgac 4141 aagggcaagt tcatcaagct gtataccatt gacaacctgg atgagttcga caatagcgat 4201 tttatcaacc ggaatctgag cgatacctcc tacattacaa acgccctggt gaatcatctg 4261 acttttagca attccaagta caagtactct gtggtcagtg tgaacggaaa gcagacctcc 4321 aatctgagaa accagatcgc tttcgtcggc atcaagaata acaaggagac agaacgcgag 4381 tggaagcgac ctgaggggtt caaatcaatc aacagcaatg actttctgat tagagaggaa 4441 ggaaagaatg acgtgaaaga cgatgtcctg atcaaggata ggagcttcaa cggccaccat 4501 gcagaggatg cctacttcat cactatcatc tctcagtact tcaggagttt taagaggatt 4561 gagcgcctga acgtgaatta ccggaaagaa accagagagc tggacgatct ggagaagaat 4621 aacatcaagt tcaaagaaaa ggcctccttc gacaactttc tgctgattaa tgctctggat 4681 gaactgaacg agaagctgaa tcagatgcga ttttctcgga tggtcatcac taagaaaaac 4741 acccagctgt tcaatgagac actgtacagc ggaaagtatg acaaaggcaa gaacactatc 4801 aagaaagtcg aaaaactgaa cctgctggac aatagaaccg ataagattaa gaaaatcgag 4861 gagttcttcg acgaggataa actgaaggaa aatgagctga ccaagctgca catcttcaac 4921 catgataaga atctgtatga gacactgaaa atcatttgga acgaagtgaa gatcgagatc 4981 aagaacaaga atctgaacga gaagaattac ttcaagtact tcgtgaacaa gaaactgcag 5041 gaggggaaga tcagcttcaa tgaatgggtc cctattctgg acaacgattt caagatcatc 5101 agaaagatca ggtacatcaa gttctcctct gaggaaaaag aaaccgacga gatcattttc 5161 tctcagagta attttctgaa gatcgatcag agacagaact tctcctttca caatacactg 5221 tactgggtgc agatttgggt ctataaaaac cagaaggacc agtactgttt catcagcatt 5281 gatgcccgga acagcaaatt tgaaaaggac gagatcaaga ttaactacga gaagctgaaa 5341 acccagaaag aaaagctgca gatcattaac gaggaaccaa ttctgaaaat caataagggc 5401 gacctgttcg agaacgagga aaaggaactg ttttatatcg tggggaggga tgagaaaccc 5461 cagaagctgg aaattaagta catcctggga aagaaaatca aagatcagaa gcagattcag 5521 aagcccgtga agaaatactt ccctaattgg aagaaggtca atctgacata catgggcgag 5581 atcttcaaga aataagaatt cggtaccgag ctcaagcttg cggccgccta tagtgtcacc 5641 taaatcgtaa cccagctttc ttgtacaaag tggtcgggtg gcatccctgt gacccctccc 5701 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa 5761 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg 5821 gggtggtatg gagcaagggg caagttggga tgacaacctg tagggcctgc ggggtctatt 5881 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg 5941 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca 6001 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 6061 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac 6121 aggcgtgaac cactgctccc ttccctgtcc ttctagcata accccttggg gcctctaaac 6181 gggtcttgag gggttttttg tctagaagac aatagcaggc atgctgggga tgcggtgggc 6241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 6301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 6361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 6421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 6481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 6541 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 6601 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 6661 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 6721 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 6781 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 6841 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 6901 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 6961 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 7021 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 7081 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 7141 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 7201 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 7261 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 7321 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 7381 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 7441 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 7501 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 7561 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 7621 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 7681 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 7741 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 7801 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 7861 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 7921 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 7981 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 8041 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 8101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 8161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 8221 tcatgtctgt tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt 8281 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg 8341 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat 8401 atgtaactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa 8461 gcatgcggag gaaattctcc ttgaagtttc cctggtgttc aaagtaaagg agtttgcacc 8521 agacgcacct ctgttcactg gtccggcgta ttaaaacacg atacattgtt attagtacat 8581 ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa 8641 taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc 8701 agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg 8761 attagtttca aacaagggtt gtttttccga accgatggct ggactatcta atggattttc 8821 gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt 8881 ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt 8941 tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa atagagcttg 9001 gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc 9061 ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat 9121 tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttgcgatcgc 9181 ataacttcgt atagcataca ttatacgaag ttataagctc ggaacgctgc gctcggtcgt 9241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 9301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 9361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc ctgacgagca tcacaaaaat 9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9541 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 9961 ggatctcaag aagatccttt gttaccaatg cttaatcagt gaggcaccta tctcagcgat 10021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 10081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 10141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 10201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 10261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 10321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 10381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 10441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 10501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 10561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 10621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 10681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 10741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 10801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10861 ttattgaagc atttatcagg gttattgtct catgtccgcg cgtt //