| 出品公司: | Addgene |
|---|---|
| 载体名称: | PX388 或 PX388 Bacteroides coprophilus Cas9 |
| 质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 11723 bp |
| 5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | NLS (C ter)；HA、NLS (N ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 昆虫细胞 |
| 备注: | -- |
| 产品目录号: | #68325 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
PX388载体质粒基本信息
PX388质粒图谱载体图谱和PX388载体序列质粒序列多克隆位点信息
PX388质粒载体简介
PX388质粒序列载体序列
LOCUS PX388 Bacteroides coprophilus Cas9 11723 bp DNA SYN
DEFINITION PX388 Bacteroides coprophilus Cas9
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11723
/organism="PX388 Bacteroides coprophilus Cas9"
/mol_type="other DNA"
misc_feature 47..1001
/label="Ac_5_flank"
CDS complement(343..948)
/label="ORF frame 3"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1023..1587
/label="CMV_immearly_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1090..1377
/label="CAG_enhancer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1544..1564
/label="CMV_fwd_primer"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
promoter 1615..1633
/label="T7_promoter"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1633..1656
/label="lacO"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
misc_feature 1772..1796
/label="attB1"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
CDS 1822..6414
/label="ORF frame 1"
/translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
misc_feature 1825..1851
/label="HA"
/translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
misc_feature 1855..1875
/label="SV40 NLS"
/translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
misc_feature 1861..1875
/label="NLS"
/translation="MYPYDVPDYASPKKKRKVEASMKKTLGLDLGTNSIGWAVINSNI
DSEGKEKLVGISSCGSRIIPMDATTLGDFGKGNTKSPVAERTRLRGIRRLLERSLLRR
ERLHRVLSVMGFLPEHYASQLDRYGKFLPETEPKLAWYKDDSGRYQFLFQKSFHEMLE
DFRQHQPELVAGEKKIPYDWTIYYLRKKALSQEITKEELAWILLNFNQKRGYYQLRGE
EEQEENNKSVEYHALKVVSVEDSGERKGKDIWYNVTLENGWVYRRASNIPLDWTGKVK
EFVVTTELDDAGNPKKDKEGNVKRSFRAPKEDDWGLLKTRTQAQIDESGKTVGTYIYE
SLLCMPNQKIRGKLVRTIERKYYKDELRQILVKQSEFHAALQDHNLLLSCIEELYPNN
EAHRRLLSASSFIYFLIEDILFYQRPLKSQKGLIDNCPYESHIYKDKKDGSLHHVPLK
CVSKSHPLFQEFRLWQFLSNLRIYQRERMMDGSLKLDVDVTREFLPSEEDYVKLFDWL
NERKEISQKFLLAYKPFGLKKNEQANYRWNYVEDKSYPCNETRAEIKSRLSKAGVPEE
FLTEEKEEALWHILYSISDKKELTKALGTFAAKNCLNESFVEVFAKIPPFESNYAAYS
LKAIRKLLALMRMGKYWNEQAIDRQTRDRIEKILTGEYDETIRSRVREKAMLLTDISS
FRGLPLWLACYIVYDRHSESKELVKWETPADIDHFLSKFKQNSLRNPIVEQVITESLR
TVRDIWKQEGKIDEIHVELGREMKNPAKERARITAQVQENENTNLRIKALLAEFMNPE
FEIENVHPYSPGQQEILRIYEDGVLSGIAEKDLPDDITAILKKFRENDVKKRPTTSEV
LRYKLWLEQRYRSPYTGRVIPLGKLFTPAYEIEHVIPQSRYFDDSISNKVICESAVNK
LKDNCLGYEFIKKHSGEMVELGNGETVPVFSVEEYERFVKESYFGNSKKMKKLLLEDI
PDSFIERQLNDSRYISRVVTSLLSNLVCEEGEQDGLSKNVIVCTGGITDRLKKDWGVQ
EVWNRIILPRFLRLNEITGRTDFTSTSVNGHLLPALPLYLQKGFNKKRIDHRHHAMDA
IVIACANRNIVNYLNNSSARKNSEISRYDLQRLLCEKVKTDANGNYKWILRKPWETFT
QDVYAALTNIVVSFKQNLRVINRTTNYYQHYNEAGEKRMIPQTKGDRWAIRKPMHKDT
VYGEVNLRKEKTLPLRDVVKNPSIVVDKSLKNKLYELLKSQYDLKAIAKYFETHQDVW
ADVNLKKIKVYYFTKETNERFFATRKSLDPSFDQKKIEEEVTDTGVQKILLHHLQQNN
NDPDMAFSPDGIDRMNQNMTILNDGKWHQPIYKVRTYEKADKFAVGESGNKAKKFVEA
AKGTNLFFAVYESVQEDEAIGKQVCKRTFATIPLNEVIKRKKQGLPAAPEDLNGNLPK
FVLSPNDLVYLPTEEERNSSRIIQPLDRERIYKMVSSSGSQCFFIKVFVANSIWDKNE
YSSLNKMERAITNEMIKEICVPIKIDRLGNVSLIQI*"
CDS complement(2149..3189)
/label="ORF frame 3"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
misc_feature complement(6447..6464)
/label="Sp6_primer"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
misc_feature 6469..6493
/label="attB2"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
terminator 6505..6971
/label="hGH_PA_terminator"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
terminator 6977..7012
/label="T7_terminator"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
rep_origin 7129..7435
/label="f1_origin"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
misc_feature complement(7549..7569)
/label="pBABE_3_primer"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
misc_feature complement(7555..7770)
/label="SV40_enhancer"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
promoter 7567..7835
/label="SV40_promoter"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
rep_origin 7734..7811
/label="SV40_origin"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
misc_feature 7796..7815
/label="SV40pro_F_primer"
/translation="MGFADTLQRHMVQTSIFLILVDVRLVGAVIDQPFLGLQWALIKQ
NVLDQEVDEAAGAQQPSVGLIVRVQLFDTGQQQIMILQSGVELALLHQDLSQLVFIVL
AFNGPHQFSPDLLVGHTQQRLVDVSSHGLAGFVDLSLGPSFQQPPIVLLWCPKGSFHI
SFLIFLWVPGIVQLGSDHELFHLAGPVQGNVASPSIDPSVLQSHIVPDIFALSFSRVL
DAHHFQGVILHALIVLFLLFLPSQLIVAAFLVEIQQNPGQFLLSDLLRQCFLPQIVDC
PIVRDFLLSCHQFWLMLSKVLQHLVEGLLKQELIPAAIVFVPCQLGLGFWQKLSIAVQ
LASVMLWQEPHD*"
CDS 7950..8744
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 7953..8741
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(8259..8795)
/label="ORF frame 1"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 8921..9040
/label="SV40_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 9009..9028
/label="EBV_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 9231..9266
/label="T7_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature complement(9289..9306)
/label="Baculovirs_rev_primer"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
misc_feature 9296..9373
/label="3_AcPH"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
CDS complement(9403..10020)
/label="ORF frame 3"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
misc_feature 10000..10033
/label="loxP"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
gene complement(10801..11661)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
CDS complement(10801..11661)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatataccA CCGGTGCCAC CATGTACCCA TACGATGTTC CAGATTACGC TTCGCCGAAG
1861 AAAAAGCGCA AGGTCGAAGC GTCCATGAAG AAAACACTGG GACTGGACCT GGGGACTAAC
1921 AGCATTGGGT GGGCCGTCAT CAACTCAAAC ATCGACTCAG AAGGAAAAGA AAAGCTGGTG
1981 GGGATCAGCT CCTGCGGAAG CCGGATCATT CCTATGGACG CTACCACACT GGGAGATTTC
2041 GGAAAGGGCA ACACTAAAAG TCCAGTGGCA GAGCGAACCC GACTGCGGGG CATTCGGAGA
2101 CTGCTGGAAA GGTCACTGCT GAGGCGCGAG CGACTGCACC GAGTGCTGTC AGTCATGGGG
2161 TTCCTGCCAG AGCATTACGC TAGCCAGCTG GACCGCTATG GAAAGTTTCT GCCAGAAACC
2221 GAGCCCAAGC TGGCATGGTA CAAAGACGAT AGCGGCAGGT ATCAGTTCCT GTTTCAGAAG
2281 TCCTTCCACG AGATGCTGGA GGACTTTCGA CAGCATCAGC CAGAACTGGT GGCAGGAGAG
2341 AAGAAAATCC CTTACGATTG GACAATCTAC TATCTGCGGA AGAAAGCACT GTCTCAGGAG
2401 ATCACTAAGG AGGAACTGGC CTGGATTCTG CTGAATTTCA ACCAGAAACG CGGCTACTAT
2461 CAGCTGCGAG GGGAGGAAGA GCAGGAAGAG AACAATAAGA GCGTGGAGTA TCACGCCCTG
2521 AAAGTGGTGA GCGTCGAGGA CTCTGGAGAA AGAAAGGGCA AAGATATCTG GTACAATGTG
2581 ACTCTGGAGA ACGGATGGGT CTATCGACGG GCTAGCAACA TTCCCCTGGA CTGGACCGGC
2641 AAGGTGAAAG AGTTCGTGGT CACTACCGAG CTGGACGATG CCGGGAACCC AAAGAAAGAT
2701 AAGGAAGGAA ATGTGAAAAG ATCCTTTAGG GCACCAAAGG AGGACGATTG GGGGCTGCTG
2761 AAAACTAGGA CCCAGGCTCA GATCGACGAA TCCGGCAAGA CCGTGGGAAC TTACATCTAC
2821 GAGTCTCTGC TGTGTATGCC CAACCAGAAG ATCCGGGGAA AACTGGTGAG GACCATTGAA
2881 CGCAAGTACT ATAAAGACGA GCTGAGACAG ATCCTGGTGA AGCAGAGCGA GTTCCACGCC
2941 GCTCTGCAGG ATCATAATCT GCTGCTGTCC TGTATCGAAG AGCTGTACCC TAACAATGAG
3001 GCCCACAGAA GGCTGCTGAG CGCCAGCAGC TTCATCTACT TCCTGATCGA GGACATTCTG
3061 TTTTATCAGC GCCCACTGAA GTCCCAGAAA GGGCTGATCG ATAACTGCCC CTACGAGTCT
3121 CACATCTACA AGGATAAGAA AGATGGAAGT CTGCACCATG TGCCTCTGAA GTGTGTCAGC
3181 AAATCCCATC CACTGTTCCA GGAATTTCGC CTGTGGCAGT TCCTGTCTAA CCTGCGAATC
3241 TACCAGAGAG AGAGGATGAT GGACGGCAGT CTGAAACTGG ACGTGGATGT CACCCGGGAG
3301 TTCCTGCCCT CAGAAGAGGA CTACGTGAAG CTGTTTGATT GGCTGAATGA GAGAAAGGAA
3361 ATCTCTCAGA AATTCCTGCT GGCTTATAAA CCTTTTGGGC TGAAGAAAAA CGAACAGGCA
3421 AATTACAGAT GGAACTATGT GGAGGACAAG AGCTACCCCT GCAACGAGAC ACGGGCAGAA
3481 ATCAAGAGCA GACTGTCCAA AGCCGGAGTG CCTGAAGAGT TTCTGACTGA AGAGAAGGAA
3541 GAGGCCCTGT GGCACATCCT GTATTCTATT AGTGATAAGA AAGAGCTGAC TAAGGCTCTG
3601 GGCACCTTCG CAGCCAAAAA CTGTCTGAAT GAGTCTTTCG TGGAAGTCTT TGCCAAGATC
3661 CCCCCTTTTG AGTCAAACTA CGCTGCATAT AGCCTGAAGG CTATTAGGAA ACTGCTGGCA
3721 CTGATGCGCA TGGGGAAGTA CTGGAATGAA CAGGCCATCG ACAGGCAGAC TCGCGATCGA
3781 ATCGAGAAAA TTCTGACCGG AGAGTATGAC GAAACAATCC GGAGCAGAGT GAGGGAGAAG
3841 GCAATGCTGC TGACCGATAT TAGCAGCTTC CGGGGCCTGC CTCTGTGGCT GGCCTGTTAC
3901 ATCGTGTATG ACCGCCACTC AGAGAGCAAG GAACTGGTCA AATGGGAGAC ACCAGCCGAC
3961 ATCGATCATT TCCTGTCCAA GTTTAAACAG AACAGCCTGC GGAACCCCAT CGTGGAACAG
4021 GTCATTACAG AGTCCCTGCG CACTGTGCGA GACATTTGGA AGCAGGAGGG AAAAATCGAT
4081 GAGATTCACG TGGAACTGGG CCGGGAGATG AAGAACCCTG CAAAAGAGCG CGCCCGAATT
4141 ACAGCTCAGG TGCAGGAAAA TGAGAACACT AATCTGAGAA TCAAGGCTCT GCTGGCAGAG
4201 TTCATGAACC CCGAATTTGA GATTGAAAAT GTGCATCCAT ACTCACCCGG CCAGCAGGAA
4261 ATCCTGCGGA TCTACGAGGA CGGCGTGCTG AGCGGGATCG CTGAGAAGGA TCTGCCTGAC
4321 GATATCACAG CAATTCTGAA GAAATTCCGA GAAAACGACG TGAAGAAACG GCCAACAACT
4381 AGCGAAGTCC TGCGGTACAA ACTGTGGCTG GAGCAGCGGT ACAGATCCCC ATATACCGGA
4441 AGAGTGATCC CCCTGGGCAA GCTGTTCACA CCTGCTTACG AGATCGAACA CGTGATTCCC
4501 CAGAGCCGGT ATTTTGACGA TTCCATCTCT AACAAAGTGA TCTGCGAAAG TGCCGTCAAT
4561 AAGCTGAAAG ATAACTGTCT GGGCTATGAG TTCATCAAGA AACATTCCGG GGAGATGGTG
4621 GAACTGGGGA ATGGAGAGAC AGTGCCCGTG TTCAGCGTGG AAGAGTACGA ACGGTTCGTC
4681 AAGGAGTCTT ACTTCGGCAA CAGTAAGAAA ATGAAGAAAC TGCTGCTGGA GGACATCCCA
4741 GATAGCTTCA TTGAGAGACA GCTGAATGAC AGTCGATACA TCTCACGGGT GGTCACATCT
4801 CTGCTGAGTA ACCTGGTGTG CGAAGAGGGA GAGCAGGATG GCCTGTCCAA GAATGTGATC
4861 GTCTGTACCG GCGGGATTAC AGACAGGCTG AAGAAAGATT GGGGAGTGCA GGAAGTCTGG
4921 AACCGCATCA TTCTGCCTCG GTTCCTGAGA CTGAATGAGA TCACCGGACG GACAGACTTT
4981 ACAAGTACTT CAGTGAACGG CCACCTGCTG CCTGCCCTGC CACTGTACCT GCAGAAGGGC
5041 TTTAATAAGA AAAGAATTGA CCATAGGCAC CATGCCATGG ATGCTATCGT GATTGCCTGC
5101 GCTAACCGGA ATATCGTCAA CTACCTGAAC AATTCCTCTG CTAGAAAGAA CAGCGAAATT
5161 AGCCGATATG ACCTGCAGCG GCTGCTGTGT GAGAAGGTGA AAACCGATGC CAACGGCAAT
5221 TACAAGTGGA TCCTGAGGAA ACCATGGGAG ACATTCACCC AGGATGTGTA TGCCGCTCTG
5281 ACAAACATCG TGGTCAGCTT TAAGCAGAAT CTGCGCGTGA TTAATCGAAC CACAAACTAC
5341 TATCAGCACT ACAACGAGGC AGGGGAAAAG CGCATGATCC CTCAGACCAA AGGCGACAGA
5401 TGGGCCATTA GAAAGCCAAT GCATAAAGAT ACTGTGTATG GCGAAGTCAA TCTGAGAAAG
5461 GAGAAAACCC TGCCACTGAG GGACGTGGTC AAGAACCCCA GTATCGTGGT CGATAAATCA
5521 CTGAAGAACA AGCTGTACGA GCTGCTGAAG AGCCAGTATG ACCTGAAGGC AATCGCCAAA
5581 TACTTCGAGA CACACCAGGA CGTGTGGGCA GATGTCAACC TGAAGAAAAT TAAGGTGTAC
5641 TACTTCACTA AGGAGACAAA CGAAAGATTC TTTGCCACTA GGAAGAGCCT GGACCCATCC
5701 TTTGATCAGA AGAAAATCGA AGAGGAAGTG ACTGATACCG GCGTCCAGAA GATTCTGCTG
5761 CACCATCTGC AGCAGAACAA TAACGACCCT GATATGGCCT TTTCCCCAGA CGGCATCGAT
5821 AGGATGAACC AGAATATGAC CATTCTGAAT GACGGGAAGT GGCACCAGCC CATCTACAAA
5881 GTGCGCACAT ATGAAAAGGC AGATAAATTC GCCGTCGGCG AGTCTGGGAA CAAGGCCAAG
5941 AAATTTGTGG AAGCAGCCAA AGGCACCAAT CTGTTCTTTG CCGTGTACGA GAGTGTCCAG
6001 GAGGACGAAG CTATCGGGAA GCAGGTCTGC AAACGGACAT TCGCCACTAT CCCCCTGAAC
6061 GAAGTGATCA AGAGAAAGAA ACAGGGCCTG CCCGCTGCAC CTGAGGACCT GAACGGGAAT
6121 CTGCCCAAGT TTGTGCTGAG CCCTAACGAT CTGGTCTACC TGCCCACCGA GGAAGAGAGG
6181 AATAGTTCAC GCATCATTCA GCCTCTGGAC AGGGAGCGCA TCTATAAGAT GGTGAGCTCC
6241 TCTGGGAGTC AGTGCTTCTT TATCAAAGTG TTCGTCGCCA ATTCAATTTG GGATAAGAAC
6301 GAATACAGCA GCCTGAACAA GATGGAGAGG GCTATTACAA ACGAAATGAT CAAGGAGATT
6361 TGTGTGCCTA TCAAAATTGA CCGCCTGGGC AATGTCAGCC TGATCCAGAT TTAAGAATTC
6421 ggtaccgagc tcaagcttgc ggccgcctat agtgtcacct aaatcgtaac ccagctttct
6481 tgtacaaagt ggtcgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
6541 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
6601 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
6661 aagttgggat gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
6721 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
6781 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
6841 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
6901 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
6961 tccctgtcct tctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt
7021 ctagaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
7081 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
7141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
7201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
7261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
7321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
7381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
7441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
7501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
7561 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
7621 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
7681 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
7741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
7801 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
7861 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
7921 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
7981 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
8041 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
8101 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
8161 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
8221 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
8281 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
8341 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
8401 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
8461 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
8521 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
8581 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
8641 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
8701 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
8761 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
8821 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
8881 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
8941 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
9001 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtt catgaagccc
9061 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
9121 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
9181 aatgagtatt tggtttagag tttggcaaca tatgcccata tgtaactagc ataacccctt
9241 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag catgcggagg aaattctcct
9301 tgaagtttcc ctggtgttca aagtaaagga gtttgcacca gacgcacctc tgttcactgg
9361 tccggcgtat taaaacacga tacattgtta ttagtacatt tattaagcgc tagattctgt
9421 gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa atttataatc
9481 tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt
9541 aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg
9601 tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc
9661 caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa
9721 aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt
9781 gtacaattga ctcgacgtaa acacgttaaa tagagcttgg acatatttaa catcgggcgt
9841 gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc
9901 cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc
9961 gatcaaattt gtagttgagc tttttggaat tgcgatcgca taacttcgta tagcatacat
10021 tatacgaagt tataagctcg gaacgctgcg ctcggtcgtt cggctgcggc gagcggtatc
10081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
10141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
10201 tttccatagg ctccgccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
10261 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
10321 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
10381 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
10441 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
10501 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
10561 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
10621 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
10681 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10741 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
11041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
11101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
11161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
11221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
11281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
11341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
11401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
11461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
11521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
11581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
11641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
11701 ttattgtctc atgtccgcgc gtt
//
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