| 出品公司: | Addgene |
|---|---|
| 载体名称: | pAAV-TRE-Syn-Fluc |
| 质粒类型: | 腺病毒载体；四环素诱导载体；荧光素酶报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | human Synapsin 1 |
| 载体大小: | 8361 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 萤火虫荧光素酶、橙色荧光蛋白 |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 神经细胞 |
| 备注: | pAAV-TRE-Syn-Fluc载体含有human synapsin 1 启动子，适合在神经细胞中表达。 |
| 产品目录号: | #22907 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pAAV-TRE-Syn-Fluc载体质粒基本信息
pAAV-TRE-Syn-Fluc质粒图谱载体图谱和pAAV-TRE-Syn-Fluc载体序列质粒序列多克隆位点信息
pAAV-TRE-Syn-Fluc质粒载体简介
pAAV-TRE-Syn-Fluc质粒序列载体序列
LOCUS KC152481 8361 bp DNA circular SYN 03-JUN-2013
DEFINITION Expression vector pAAV-TRE-Syn-Fluc, complete sequence.
ACCESSION KC152481
VERSION KC152481.1 GI:507593968
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-TRE-Syn-Fluc|
AUTHORS Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
TITLE RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
JOURNAL Mol Ther Nucleic Acids 2, E85 (2013)
PUBMED 23571608
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8361)
AUTHORS Chen,Y. and Sprengel,R.
TITLE Direct Submission
JOURNAL Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
Germany
FEATURES Location/Qualifiers
source 1..8361
/organism="Expression vector pAAV-TRE-Syn-Fluc"
/mol_type="other DNA"
/db_xref="taxon:1337870"
repeat_region 19..162
/note="ITR"
/rpt_type=inverted
/rpt_type=terminal
misc_feature 245..542
/note="tetracycline responsive element: TRE"
regulatory 543..559
/regulatory_class="promoter"
/note="CMVmin"
misc_feature 616..748
/note="polyadenylation signal/transcriptional pause"
regulatory 739..1190
/regulatory_class="promoter"
/note="Homo sapiens synapsin-1 promoter: Syn"
misc_feature 1195..2847
/note="firefly luciferase"
misc_feature 2848..2901
/note="2A peptide from Thosea asigna virus"
misc_feature 2914..3762
/note="tTSKid"
misc_feature 2914..3546
/note="Tet repressor: tetR[B/E]"
misc_feature 3547..3564
/note="nuclear localization signal: NLS"
misc_feature 3571..3756
/note="KRAB (Kruppel-associated box) domain; from Rat zinc
finger protein (kid-1)"
misc_feature 3763..3816
/note="2A peptide from Thosea asigna virus"
gene 3829..4485
/gene="mKO"
CDS 3829..4485
/gene="mKO"
/note="fluorescent protein"
/codon_start=1
/transl_table=11
/product="monomeric Kusabira-orange"
/protein_id="AGM53873.1"
/db_xref="GI:507593969"
/translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLR
VTMAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGG
SASVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDV
TMYLKLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS
"
ncRNA 4516..5112
/ncRNA_class="other"
/product="post-transcriptional regulatory element: WPRE"
misc_feature 5145..5355
/note="bovine growth hormone polyA signal: bGH pA"
repeat_region complement(5365..5508)
/note="ITR"
/rpt_type=inverted
/rpt_type=terminal
gene 6697..7359
/gene="Bla"
CDS 6697..7359
/gene="Bla"
/note="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AGM53874.1"
/db_xref="GI:507593970"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
HW"
ORIGIN
1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
181 ttctagtcct agggatatct tcgaaccatg gttaattaag tttaaacatc gatcaattcg
241 agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
301 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
361 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
421 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
481 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
541 acctatataa gcagagctcg gtacccgggg atcctctaga aatattaagg tacgggaggt
601 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
661 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
721 gcaaaatagg ctgtccccag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
781 tgcctacctg acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat
841 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
901 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
961 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
1021 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
1081 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
1141 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga attcatggaa
1201 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct
1261 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
1321 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
1381 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
1441 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
1501 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
1561 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
1621 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
1681 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
1741 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact
1801 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
1861 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
1921 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
1981 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
2041 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
2101 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
2161 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
2221 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
2281 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
2341 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
2401 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
2461 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
2521 aaatacaaag gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc
2581 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
2641 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
2701 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
2761 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
2821 aagaagggcg gaaagtccaa attgctcgag ggcagaggaa gtcttctaac atgcggtgac
2881 gtggaggaga atcccggccc tgcaccggga tccatgtcca gattagataa aagtaaagtg
2941 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
3001 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcacgtgcg caacaagcag
3061 actcttatga acatgctttc agaggcaata ctggcgaagc atcacacccg ttcagcaccg
3121 ttaccgactg agagttggca gcagtttctc caggaaaatg ctctgagttt ccgtaaagca
3181 ttactggtcc atcgtgatgg agcccgattg catataggga cctctcctac gcccccccag
3241 tttgaacaag cagaggcgca actacgctgt ctatgcgatg cagggttttc ggtcgaggag
3301 gctcttttca ttctgcaatc tatcagccat tttacgttgg gtgcagtatt agaggagcaa
3361 gcaacaaacc agatagaaaa taatcatgtg atagacgctg caccaccatt attacaagag
3421 gcatttaata ttcaggcgag aacctctgct gaaatggcct tccatttcgg gctgaaatca
3481 ttaatatttg gattttctgc acagttagat gaaaaaaagc atacacccat tgaggatggt
3541 aataaaccaa aaaagaagag aaagctagca gtgtcagtga catttgaaga tgtggctgtg
3601 ctctttactc gggacgagtg gaagaagctg gatctgtctc agagaagcct gtaccgtgag
3661 gtgatgctgg agaattacag caacctggcc tccatggcag gattcctgtt taccaaacca
3721 aaggtgatct ccctgttgca gcaaggagag gacccgtatc tcgagggcag aggaagtctt
3781 ctaacatgcg gtgacgtgga ggagaatccc ggccctgcac cgggatccat ggtgagtgtg
3841 attaaaccag aaatgaagat gaggtactac atggacggct ccgtcaatgg gcatgagttc
3901 acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat gacactacgc
3961 gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc
4021 tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta tttcaaacaa
4081 gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg tgggtccgct
4141 tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc caaatttact
4201 ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga ttgggagcca
4261 tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac gatgtaccta
4321 aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa
4381 aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag gaaaaccgaa
4441 ggcaacatta ctgagctggt agaagatgca gtagctcatt cctgatgtac aagtaaagcg
4501 gccgcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
4561 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
4621 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
4681 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
4741 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
4801 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
4861 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
4921 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
4981 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
5041 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
5101 ccccgcctga tcgataccgt cgactagagc tcgctgatca gcctcgactg tgccttctag
5161 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
5221 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
5281 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
5341 caggcatgct ggggagagat ctgaggaacc cctagtgatg gagttggcca ctccctctct
5401 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
5461 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaacc cccccccccc
5521 cccccctgca gcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
5581 gcgtagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg
5641 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
5701 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
5761 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
5821 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
5881 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
5941 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
6001 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
6061 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
6121 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
6181 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
6241 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
6301 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
6361 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
6421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
6481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
6541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
6601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
6661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
6721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
6781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
6841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
6901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
6961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
7021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
7081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
7141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
7201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
7261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
7321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
7381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
7441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
7501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
7561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
7621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
7681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
7741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
7801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
7861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
7921 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
7981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
8041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
8101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
8161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
8221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
8281 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
8341 attcattaat gcaggctgca g
//
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