pAcGFP1-Actin

价格：4800元

联系方式：I47-825O-882O

相关技术服务：质粒构建    基因合成    质粒大提

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载体基本信息

出品公司: Clontech
载体名称: pAcGFP1-Actin
质粒类型: 哺乳动物细胞表达载体；荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶，多克隆位点
启动子: CMV IE
载体大小: 5820 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: AcGFP1 (N-末端）
载体抗性: 卡那霉素
筛选标记: 新霉素（Neomycin）
克隆菌株: DH5α
宿主细胞（系）: 常规细胞系（293、CV-1、CHO等）
备注: 哺乳动物载体pAcGFP1-Actin组成型表达肌动蛋白，用来荧光标记细胞骨架；
AcGFP1与GFP相比，密码子经过了优化，更适合在哺乳动物细胞中高水平表达，同时也增强了亮度。
产品目录号: 632453
稳定性: 稳表达 或 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体图谱质粒图谱和多克隆位点信息

pAcGFP1-Actin载体图谱



pAcGFP1-Actin 载体特征1
pAcGFP1-Actin 载体特征2

载体简介

pAcGFP1-Actin载体描述

pAcGFP1-Actin encodes a green fluorescent protein (GFP) from Aequorea coerulescens (Excitation maximum = 475 nm; emission maximum = 505 nm) and the gene encoding human cytoplasmic β-actin (1). SV40 polyadenylation signals downstream of the AcGFP1-Actin fusion direct proper processing of the 3' end of the AcGFP1 mRNA. AcGFP1 contains silent mutations that create an open reading frame comprised almost entirely of optimized human codons. These changes increase the translational efficiency of the AcGFP1 mRNA and consequently the expression of AcGFP1 in mammalian and plant cells. The vector backbone also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen. A neomycin resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV-TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. The pAcGFP1-Actin backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

The pAcGFP1-Actin Vector expresses the AcGFP1-Actin fusion protein in mammalian cells. The protein is incorporated into growing actin filaments and allows for visualization of actin-containing subcellular structures in living and fixed cells (2, 3). This vector is not intended to be used as a cloning vector; however, unique restriction sites at the 5' end of AcGFP1, and between AcGFP1 and the β-actin open reading frame, allow excision or insertion of DNA. pAcGFP1-Actin can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (4).

Propagation in E. coli
 Suitable host strains: DH5α, HB101, and other general purpose strains. Single-stranded DNA production requires
a host containing an F plasmid such as JM109 or XL1-Blue.
 Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) to E. coli hosts.
 E. coli replication origin: pUC; copy number: ≈500
 Plasmid incompatibility group: pMB1/ColE1

载体序列

LOCUS       pAcGFP1-Actin	5820 bp 	DNA	SYN
DEFINITION  pAcGFP1-Actin
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="pAcGFP1-Actin"
                     /mol_type="other DNA"
     promoter        10..562
                     /label="CMV_immearly_promoter"
     misc_feature    65..352
                     /label="CAG_enhancer"
     misc_feature    519..539
                     /label="CMV_fwd_primer"
     promoter        520..589
                     /label="CMV_promoter"
     CDS             613..2478
                     /label="ORF frame 1"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYKSGLRSRAMDDDIAALVVDNGSGMCKAGFAGDDAPRAV
                     FPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHH
                     TFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASG
                     RTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTA
                     EREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALF
                     QPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL
                     APSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF*"
     gene            616..1329
                     /label="AcGFP1"
                     /gene="AcGFP1"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYKSGLRSRAMDDDIAALVVDNGSGMCKAGFAGDDAPRAV
                     FPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHH
                     TFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASG
                     RTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTA
                     EREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALF
                     QPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITAL
                     APSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF*"
     CDS             complement(1768..2517)
                     /label="ORF frame 1"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     terminator      2501..2729
                     /label="SV40_PA_terminator"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     misc_feature    2699..2718
                     /label="EBV_rev_primer"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     rep_origin      complement(2868..3174)
                     /label="f1_origin"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     promoter        3253..3281
                     /label="AmpR_promoter"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     misc_feature    complement(3347..3367)
                     /label="pBABE_3_primer"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     misc_feature    complement(3353..3568)
                     /label="SV40_enhancer"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     promoter        3365..3633
                     /label="SV40_promoter"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     rep_origin      3532..3609
                     /label="SV40_origin"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     misc_feature    3594..3613
                     /label="SV40pro_F_primer"
                     /translation="MADYDQLSRSGGSLEAFAVDDGGAGLVILLLADPHLLEGGQRGQ
                     DGAADPHGVLALRRSNDLDLHCAGCQGSDLLLHPVGNARVHGGAARQHCVGVQVFADV
                     HVTLHDGVEGSFVDATGLHAQEGRLEECLRAAEPLIANGDDLAVRQLVALLQGGAGSS
                     RGHLLLEVQGDVAQLLLNVTHDFPLGRGGEAVAALGEDLHEVVSQVPASQVQTQDGMG
                     EGIPLVDGHSVGDPVTGVHHDASGTARGVQG*"
     CDS             3716..4510
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            3719..4507
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(4025..4561)
                     /label="ORF frame 3"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      4685..4954
                     /label="TK_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     rep_origin      5102..5721
                     /label="pBR322_origin"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
ORIGIN
    1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG
   61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT
  121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA
  181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC
  241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA
  301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC
  361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG
  421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG
  481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT
  541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA
  601 CCGGTCGCCA CCATGGTGAG CAAGGGCGCC GAGCTGTTCA CCGGCATCGT GCCCATCCTG
  661 ATCGAGCTGA ATGGCGATGT GAATGGCCAC AAGTTCAGCG TGAGCGGCGA GGGCGAGGGC
  721 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCTGTG
  781 CCCTGGCCCA CCCTGGTGAC CACCCTGAGC TACGGCGTGC AGTGCTTCTC ACGCTACCCC
  841 GATCACATGA AGCAGCACGA CTTCTTCAAG AGCGCCATGC CTGAGGGCTA CATCCAGGAG
  901 CGCACCATCT TCTTCGAGGA TGACGGCAAC TACAAGTCGC GCGCCGAGGT GAAGTTCGAG
  961 GGCGATACCC TGGTGAATCG CATCGAGCTG ACCGGCACCG ATTTCAAGGA GGATGGCAAC
 1021 ATCCTGGGCA ATAAGATGGA GTACAACTAC AACGCCCACA ATGTGTACAT CATGACCGAC
 1081 AAGGCCAAGA ATGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGATGGCAGC
 1141 GTGCAGCTGG CCGACCACTA CCAGCAGAAT ACCCCCATCG GCGATGGCCC TGTGCTGCTG
 1201 CCCGATAACC ACTACCTGTC CACCCAGAGC GCCCTGTCCA AGGACCCCAA CGAGAAGCGC
 1261 GATCACATGA TCTACTTCGG CTTCGTGACC GCCGCCGCCA TCACCCACGG CATGGATGAG
 1321 CTGTACAAGT CCGGACTCAG ATCTCGAGCT ATGGATGATG ATATCGCCGC GCTCGTCGTC
 1381 GACAACGGCT CCGGCATGTG CAAGGCCGGC TTCGCGGGCG ACGATGCCCC CCGGGCCGTC
 1441 TTCCCCTCCA TCGTGGGGCG CCCCAGGCAC CAGGGCGTGA TGGTGGGCAT GGGTCAGAAG
 1501 GATTCCTATG TGGGCGACGA GGCCCAGAGC AAGAGAGGCA TCCTCACCCT GAAGTACCCC
 1561 ATCGAGCACG GCATCGTCAC CAACTGGGAC GACATGGAGA AAATCTGGCA CCACACCTTC
 1621 TACAATGAGC TGCGTGTGGC TCCCGAGGAG CACCCCGTGC TGCTGACCGA GGCCCCCCTG
 1681 AACCCCAAGG CCAACCGCGA GAAGATGACC CAGATCATGT TTGAGACCTT CAACACCCCA
 1741 GCCATGTACG TTGCTATCCA GGCTGTGCTA TCCCTGTACG CCTCTGGCCG TACCACTGGC
 1801 ATCGTGATGG ACTCCGGTGA CGGGGTCACC CACACTGTGC CCATCTACGA GGGGTATGCC
 1861 CTCCCCCATG CCATCCTGCG TCTGGACCTG GCTGGCCGGG ACCTGACTGA CTACCTCATG
 1921 AAGATCCTCA CCGAGCGCGG CTACAGCTTC ACCACCACGG CCGAGCGGGA AATCGTGCGT
 1981 GACATTAAGG AGAAGCTGTG CTACGTCGCC CTGGACTTCG AGCAAGAGAT GGCCACGGCT
 2041 GCTTCCAGCT CCTCCCTGGA GAAGAGCTAC GAGCTGCCTG ACGGCCAGGT CATCACCATT
 2101 GGCAATGAGC GGTTCCGCTG CCCTGAGGCA CTCTTCCAGC CTTCCTTCCT GGGCATGGAG
 2161 TCCTGTGGCA TCCACGAAAC TACCTTCAAC TCCATCATGA AGTGTGACGT GGACATCCGC
 2221 AAAGACCTGT ACGCCAACAC AGTGCTGTCT GGCGGCACCA CCATGTACCC TGGCATTGCC
 2281 GACAGGATGC AGAAGGAGAT CACTGCCCTG GCACCCAGCA CAATGAAGAT CAAGATCATT
 2341 GCTCCTCCTG AGCGCAAGTA CTCCGTGTGG ATCGGCGGCT CCATCCTGGC CTCGCTGTCC
 2401 ACCTTCCAGC AGATGTGGAT CAGCAAGCAG GAGTATGACG AGTCCGGCCC CTCCATCGTC
 2461 CACCGCAAAT GCTTCTAGGG ATCCACCGGA TCTAGATAAC TGATCATAAT CAGCCATACC
 2521 ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA
 2581 CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
 2641 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
 2701 GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTAAATT GTAAGCGTTA ATATTTTGTT
 2761 AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG
 2821 CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG
 2881 GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA
 2941 TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG
 3001 CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA
 3061 GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT
 3121 GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT
 3181 ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
 3241 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
 3301 ATAATATTGA AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG
 3361 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC
 3421 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
 3481 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC
 3541 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT
 3601 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT
 3661 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
 3721 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
 3781 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
 3841 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
 3901 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
 3961 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
 4021 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
 4081 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
 4141 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
 4201 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA
 4261 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG
 4321 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC
 4381 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT
 4441 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA
 4501 GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA
 4561 TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC
 4621 CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC
 4681 CCTAGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG
 4741 ACGGCAATAA AAAGACAGAA TAAAACGCAC GGTGTTGGGT CGTTTGTTCA TAAACGCGGG
 4801 GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC
 4861 GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC
 4921 GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG GTTACTCATA TATACTTTAG
 4981 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT
 5041 CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA
 5101 AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA
 5161 AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT
 5221 CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG
 5281 TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC
 5341 CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA
 5401 CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC
 5461 AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC
 5521 GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA
 5581 GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
 5641 TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA
 5701 TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT
 5761 CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCATGCAT

//

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