载体名称: | pCAMBIA1300-nLuc |
---|---|
载体又名: | pCambia-nLuc , pCambia1300nluc |
质粒类型: | 植物双分子荧光质粒 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 多克隆位点 |
复制子: | pVS1V |
载体大小: | 11752 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | nluc |
载体抗性: | 卡那霉素 |
筛选标记: | Hyg |
克隆菌株: | Stbl3 |
备注: | -- |
产品目录号: | 17352 |
买家导航
pCAMBIA1300-nLuc载体 pCambia-nLuc pCAMBIA1300nLuc质粒基本信息
pCAMBIA1300-nLuc质粒图谱 pCambia-nLuc pCAMBIA1300nLuc载体图谱和pCAMBIA1300-nLuc载体序列 pCambia-nLuc pCAMBIA1300nLuc质粒序列多克隆位点信息
pCAMBIA1300-nLuc质粒 pCambia-nLuc pCAMBIA1300nLuc载体简介
pCAMBIA1300-nLuc质粒序列 pCambia-nLuc pCAMBIA1300nLuc载体序列
LOCUS pCambia1300-nLuc 11752 bp ds-DNA circular SYN 28-01-2019 DEFINITION . ACCESSION . VERSION . KEYWORDS pCambia1300-nLuc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11752) AUTHORS Triple Threat TITLE Direct Submission JOURNAL Exported from SnapGene 1.1.3 http://www.biofeng.com FEATURES Location/Qualifiers source 1..11752 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 500..845 /note="CaMV 35S promoter" /note="color: #ffffff; direction: RIGHT" misc_feature 854..921 /note="MCS" /note="color: #ffff99" CDS 1035..2198 /codon_start=1 /note="nLuc" /note="color: #ffff99" terminator 2205..2846 /note="E9 terminator" /note="color: #ffffff" misc_feature 3073..3097 /note="RB T-DNA repeat" /note="color: #ffe4c4" CDS 4397..5026 /codon_start=1 /note="pVS1 StaA" /note="color: #993366" CDS 5460..6527 /codon_start=1 /note="pVS1 RepA" /note="color: #993366" rep_origin 6593..6787 /note="pVS1 oriV" /note="color: #ffff00" misc_feature 7131..7271 /note="bom" /note="color: #a6acb3" rep_origin complement(7457..8045) /direction=LEFT /note="ori" /note="color: #ffff00" CDS complement(8132..8926) /codon_start=1 /note="KanR" /note="color: #ccffcc" misc_feature 9351..9375 /note="LB T-DNA repeat" /note="color: #ffe4c4" polyA_signal 9453..9627 /note="CaMV poly(A) signal" /note="color: #a6acb3" CDS complement(9667..10692) /codon_start=1 /note="HygR" /note="color: #ccffcc" promoter complement(10760..11437) /note="CaMV 35S promoter (enhanced)" /note="color: #ffffff; direction: LEFT" ORIGIN 1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta 61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa 121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca 181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc 241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc 301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg 361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct 421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct 481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg 541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct 601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca 661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa 721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac 781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga 841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagtg 901 tcgaccagat ctcgtacgcg tcccggggcg gtggctcatc tggcggaggt gaagacgcca 961 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc 1021 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg 1081 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag 1141 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact 1201 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg 1261 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg 1321 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa 1381 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt 1441 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt 1501 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc 1561 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc 1621 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc 1681 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa 1741 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg 1801 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg 1861 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg 1921 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga 1981 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag 2041 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta 2101 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc 2161 cggaagcgac caacgccttg attgacaagg atggatgact gcagagcttt cgttcgtatc 2221 atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa 2281 tcctactgag ttcgagtatt atggcattgg gaaacatgtt tttcttgtac catttgttgt 2341 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg 2401 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg 2461 ttttttctct tatttgttgt gtgttgaatt tgaaaatata agagatatgc aaacattttg 2521 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa 2581 aacacttgta gttgtaccat tatgcttatt cactaggcaa caaatatatt ttcagaccta 2641 gaaaagctgc aaatgttact gaatacaagt atgtcctctt gtgttttaga catttatgaa 2701 ctttccttta tgtaattttc cagaatcctt gtcagattct aatcattgct ttataattat 2761 agttatactc atggatttgt agttgagtat gaaaatattt tttaatgcat tttatgactt 2821 gccaattgat tgacaacatg catcaagctt ggcactggcc gtcgttttac aacgtcgtga 2881 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 2941 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 3001 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa 3061 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta 3121 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca 3181 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat 3241 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag 3301 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt 3361 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag 3421 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc 3481 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc 3541 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac 3601 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt 3661 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac 3721 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag 3781 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg 3841 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc 3901 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc 3961 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt 4021 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc 4081 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg 4141 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct 4201 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc 4261 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg 4321 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa 4381 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa 4441 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt 4501 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc 4561 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg 4621 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat 4681 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac 4741 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc 4801 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc 4861 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc 4921 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg 4981 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa 5041 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc 5101 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag 5161 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt 5221 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat 5281 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac 5341 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg 5401 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg 5461 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga 5521 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg 5581 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg 5641 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga 5701 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc 5761 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg 5821 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga 5881 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc 5941 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg 6001 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg 6061 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag 6121 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga 6181 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga 6241 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg 6301 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca 6361 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa 6421 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca 6481 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga 6541 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata 6601 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc 6661 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag 6721 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct 6781 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt 6841 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa 6901 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac 6961 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa 7021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 7081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 7141 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 7201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 7261 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 8101 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat 8161 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact 8221 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt 8281 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga 8341 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct 8401 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc 8461 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta 8521 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg 8581 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga 8641 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga 8701 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat 8761 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc 8821 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt 8881 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc 8941 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa 9001 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt 9061 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca 9121 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt 9181 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc 9241 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg 9301 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata 9361 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta 9421 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt 9481 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg 9541 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg 9601 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat ccggtcggca 9661 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag 9721 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc 9781 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc 9841 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata 9901 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg 9961 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga 10021 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag 10081 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc 10141 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac 10201 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt 10261 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc 10321 cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc 10381 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc 10441 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg 10501 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc 10561 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag 10621 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc 10681 aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga gatagatttg 10741 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag 10801 aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc 10861 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct 10921 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt 10981 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat 11041 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg 11101 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac 11161 gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg 11221 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc 11281 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc 11341 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag 11401 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac 11461 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg 11521 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 11581 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag 11641 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 11701 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cg //
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