| 出品公司: | -- |
|---|---|
| 载体名称: | pSP64-Tol2-Transposase |
| 质粒类型: | 转座子载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | SP6 |
| 载体大小: | 5142bp |
| 5' 测序引物及序列: | sp6：ATTTAGGTGACACTATAGAA |
| 3' 测序引物及序列: | M13R |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pSP64-Tol2-Transposase载体质粒基本信息
pSP64-Tol2-Transposase质粒图谱载体图谱和pSP64-Tol2-Transposase载体序列质粒序列多克隆位点信息
pSP64-Tol2-Transposase质粒载体简介
pSP64-Tol2-Transposase质粒序列载体序列
LOCUS pSP64-Tol2-Transposase 5142 bp ds-DNA circular SYN 13-FEB-2019
DEFINITION synthetic circular DNA
KEYWORDS pSP64-Tol2-Transposase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5142)
AUTHORS Triple Threat
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5142
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 28..2115
/codon_start=1
/label=Transposase Tol2
/translation="MFIGPLEVTSCHIYYHNAQHLDLEIREIITVNQWKKMEEVCDSSA
AASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKMKCVLCLPLNKEISAFKSSPSNLR
KHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQLKVDSVFPVKHVSPVTVNKAILRY
IIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLRSKIAEAALIMKQKVTAAMSEVEW
IATTTDCWTARRKSFIGVTAHWINPGSLERHSAALACKRLMGSHTFEVLASAMNDIHSE
YEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEARRCESDDTDSEGCGEGSDGVEFQ
DASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQKALSNEHYKKLYRSVFGKCQALW
NKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAVDRILQICKEAGEGALRNICTSLE
VPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQLGWLLPSVHQLSLKLQRLHHSLR
YCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKFRTSWTNDETIIKRGMDYIRVHLE
PLDHKKELANSSSDDEDFFASLKPTTHEASKELDGYLACVSDTRESLLTFPAICSLSIK
TNTPLPASAACERLFSTAGLLFSPKRARLDTNNFENQLLLKLNLRFYNFEGGGKKKRKV
G"
primer_bind complement(2212..2228)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(2614..3202)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3373..4233)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4234..4338)
/gene="bla"
/label=AmpR promoter
misc_feature join(5126..5142,1..2)
/label=SP6 promoter
BASE COUNT 1401 a 1255 c 1227 g 1258 t
ORIGIN
1 gaatacaagc ttgggctgca ggtcgacatg ttcattggtc ctttggaagt gacgtcatgt
61 cacatctatt accacaatgc acagcacctt gacctggaaa ttagggaaat tataacagtc
121 aatcagtgga agaaaatgga ggaagtatgt gattcatcag cagctgcgag cagcacagtc
181 caaaatcagc cacaggatca agagcacccg tggccgtatc ttcgcgaatt cttttcttta
241 agtggtgtaa ataaagattc attcaagatg aaatgtgtcc tctgtctccc gcttaataaa
301 gaaatatcgg ccttcaaaag ttcgccatca aacctaagga agcatattga gagaatgcac
361 ccaaattacc tcaaaaacta ctctaaattg acagcacaga agagaaagat cgggacctcc
421 acccatgctt ccagcagtaa gcaactgaaa gttgactcag ttttcccagt caaacatgtg
481 tctccagtca ctgtgaacaa agctatatta aggtacatca ttcaaggact tcatcctttc
541 agcactgttg atctgccatc atttaaagag ctgattagta cactgcagcc tggcatttct
601 gtcattacaa ggcctacttt acgctccaag atagctgaag ctgctctgat catgaaacag
661 aaagtgactg ctgccatgag tgaagttgaa tggattgcaa ccacaacgga ttgttggact
721 gcacgtagaa agtcattcat tggtgtaact gctcactgga tcaaccctgg aagtcttgaa
781 agacattccg ctgcacttgc ctgcaaaaga ttaatgggct ctcatacttt tgaggtactg
841 gccagtgcca tgaatgatat ccactcagag tatgaaatac gtgacaaggt tgtttgcaca
901 accacagaca gtggttccaa ctttatgaag gctttcagag tttttggtgt ggaaaacaat
961 gatatcgaga ctgaggcaag aaggtgtgaa agtgatgaca ctgattctga aggctgtggt
1021 gagggaagtg atggtgtgga attccaagat gcctcacgag tcctggacca agacgatggc
1081 ttcgaattcc agctaccaaa acatcaaaag tgtgcctgtc acttacttaa cctagtctca
1141 agcgttgatg cccaaaaagc tctctcaaat gaacactaca agaaactcta cagatctgtc
1201 tttggcaaat gccaagcttt atggaataaa agcagccgat cggctctagc agctgaagct
1261 gttgaatcag aaagccggct tcagctttta aggccaaacc aaacgcggtg gaattcaact
1321 tttatggctg ttgacagaat tcttcaaatt tgcaaagaag caggagaagg cgcacttcgg
1381 aatatatgca cctctcttga ggttccaatg tttaatccag cagaaatgct gttcttgaca
1441 gagtgggcca acacaatgcg tccagttgca aaagtactcg acatcttgca agcggaaacg
1501 aatacacagc tggggtggct gctgcctagt gtccatcagt taagcttgaa acttcagcga
1561 ctccaccatt ctctcaggta ctgtgaccca cttgtggatg ccctacaaca aggaatccaa
1621 acacgattca agcatatgtt tgaagatcct gagatcatag cagctgccat ccttctccct
1681 aaatttcgga cctcttggac aaatgatgaa accatcataa aacgaggcat ggactacatc
1741 agagtgcatc tggagccttt ggaccacaag aaggaattgg ccaacagttc atctgatgat
1801 gaagattttt tcgcttcttt gaaaccgaca acacatgaag ccagcaaaga gttggatgga
1861 tatctggcct gtgtttcaga caccagggag tctctgctca cgtttcctgc tatttgcagc
1921 ctctctatca agactaatac acctcttccc gcatcggctg cctgtgagag gcttttcagc
1981 actgcaggat tgcttttcag ccccaaaaga gctaggcttg acactaacaa ttttgagaat
2041 cagcttctac tgaagttaaa tctgaggttt tacaactttg agggcggcgg caagaagaag
2101 cgcaaggtgg gctagggatc cccgggcgag ctcccaaaaa aaaaaaaaaa aaaaaaaaaa
2161 aaaaaccgaa ttctctagag gtaccccatg gctcgaggaa ttcgtaatca tgtcatagct
2221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
2281 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
2341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
2401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
2461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
2521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
2581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
2821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
3001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
3061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
3121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
3181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
3241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
3301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
3361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
3421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
3481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
3541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
3601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
3661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
3721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
3781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
3841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
3901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
3961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
4021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
4081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
4141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
4201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
4261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
4321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
4381 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
4441 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
4501 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
4561 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc
4621 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
4681 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
4741 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
4801 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
4861 cggtgatgcc ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga
4921 ttggttcgct gaccatttcc gggtgcggga cggcgttacc agaaactcag aaggttcgtc
4981 caaccaaacc gactctgacg gcagtttacg agagagatga tagggtctgc ttcagtaagc
5041 cagatgctac acaattaggc ttgtacatat tgtcgttaga acgcggctac aattaataca
5101 taaccttatg tatcatacac atacgattta ggtgacacta ta
//
