质粒名称: | pLenti X1 Puro empty (w610-1) |
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出品公司: | 爱迪基因 |
目录编号: | 20953 |
存储实验室: | Eric Campeau |
用途: | 第三代慢病毒空控制向量 |
载体骨架基本信息 |
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载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
载体抗性: | 氨苄霉素 |
空载体大小: | 7424 bp |
载体类型: | 哺乳细胞表达,慢病毒,RNAi |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | None |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Sal I(未破坏) |
3' 克隆位点: | Xba I (未破坏) |
5' 测序引物 | n/a |
文献引用方法 |
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材料和方法部分: | pLenti X1 Puro empty (w610-1) was a gift from Eric Campeau (Addgene plasmid # 20953) |
参考文献部分: |
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
pLenti X1 Puro empty (w610-1)载体质粒基本信息
pLenti X1 Puro empty (w610-1)质粒图谱载体图谱和pLenti X1 Puro empty (w610-1)载体序列质粒序列多克隆位点信息
pLenti X1 Puro empty (w610-1)质粒载体简介
pLenti X1 Puro empty (w610-1)质粒序列载体序列
LOCUS pLenti X1 Puro empty (w610-1) 7424 bp DNA SYN DEFINITION pLenti X1 Puro empty (w610-1) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7424 /organism="pLenti X1 Puro empty (w610-1)" /mol_type="other DNA" promoter 62..90 /label="AmpR_promoter" gene 132..992 /label="Ampicillin" /gene="Ampicillin" CDS 132..992 /label="ORF frame 3" rep_origin 1147..1766 /label="pBR322_origin" promoter 2075..2104 /label="lac_promoter" misc_feature 2118..2140 /label="M13_pUC_rev_primer" promoter 2139..2157 /label="M13_reverse_primer" promoter 2174..2193 /label="T3_promoter" misc_feature 2448..2628 /label="HIV-1_5_LTR" misc_feature 2448..2628 /label="truncHIV-1_3_LTR" misc_feature 2736..2780 /label="HIV-1_psi_pack" CDS 3168..3668 /label="ORF frame 3" misc_feature 3290..3523 /label="RRE" CDS 3629..4189 /label="ORF frame 2" misc_feature 4016..4031 /label="cPPT" misc_feature 4161..4185 /label="attB1" misc_feature 4251..4275 /label="attB2" misc_feature 4308..4883 /label="WPRE" CDS 4396..5025 /label="ORF frame 1" misc_feature complement(4943..4966) /label="MSCV_rev_primer" CDS 5188..6027 /label="ORF frame 1" misc_feature 5340..5359 /label="mPGK_F_primer" CDS complement(5353..6078) /label="ORF frame 3" gene 5428..6027 /label="puro (variant)" /gene="puro (variant)" misc_feature 6148..6163 /label="cPPT" misc_feature 6148..6169 /label="U3PPT" misc_feature 6218..6398 /label="HIV-1_5_LTR" misc_feature 6218..6398 /label="truncHIV-1_3_LTR" terminator 6473..6592 /label="SV40_PA_terminator" misc_feature 6561..6580 /label="EBV_rev_primer" rep_origin 6610..6687 /label="SV40_origin" misc_feature 6672..6691 /label="SV40pro_F_primer" promoter complement(6778..6796) /label="T7_promoter" promoter complement(6806..6822) /label="M13_forward20_primer" misc_feature 6815..6958 /label="lacZ_a" misc_feature complement(6815..6837) /label="M13_pUC_fwd_primer" rep_origin 6984..7290 /label="f1_origin" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tTCCAGTGTG 4141 GTGGAATTCT GCAGATATCA ACAAGTTTGT ACAAAAAAGC AGGCTTTAAA GGAACCAATT 4201 CAGTCGACTG GATCCGGTAC CGAATTCGCG GCCGCACTCG AGATATCTAG ACCCAGCTTT 4261 CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC TCGAcaatca acctctggat 4321 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 4381 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 4441 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 4501 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 4561 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 4621 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 4681 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 4741 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 4801 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 4861 acgagtcgga tctccctttg ggccgcctcc ccgcctgGAA TTCTACCGGG TAGGGGAGGC 4921 GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA 4981 CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGGCTCC 5041 GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC 5101 CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA GTAGCACGTC TCACTAGTCT 5161 CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA 5221 ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG GGAAGGGGTG GGTCCGGGGG 5281 CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA AGGTCCTCCG GAGGCCCGGC 5341 ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC TCCTCTTCCT CATCTCCGGG 5401 CCTTTCGACC TGCAGCCCAA GCTTACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC 5461 ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC 5521 GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA 5581 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC 5641 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC 5701 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC 5761 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG 5821 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC 5881 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC 5941 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG 6001 TGCATGACCC GCAAGCCCGG TGCCTGACGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA 6061 AGGAGCGCAC GACCCCATGC ATCTCGAGGG CCCGGTACct ttaagaccaa tgacttacaa 6121 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca 6181 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 6241 ctgagcctgg gagctctctg gctgcctagg gaacccactg cttaagcctc aataaagctt 6301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 6481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 6541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 6601 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 6721 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct 6781 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa 6841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 6901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 6961 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 7021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 7081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 7141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 7201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 7261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 7321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 7381 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcc //