| 质粒名称: | pLenti X1 Puro empty (w610-1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20953 |
| 存储实验室: | Eric Campeau |
| 用途: | 第三代慢病毒空控制向量 |
|
载体骨架基本信息 |
|
| 载体名称: | p156RRL-sinPPT-CMV-GFP-PRE/Nhe I |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 7424 bp |
| 载体类型: | 哺乳细胞表达,慢病毒，RNAi |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sal I(未破坏) |
| 3' 克隆位点: | Xba I (未破坏) |
| 5' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti X1 Puro empty (w610-1) was a gift from Eric Campeau (Addgene plasmid # 20953) |
| 参考文献部分: |
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体图谱.png)
载体简介
载体序列
LOCUS pLenti X1 Puro empty (w610-1) 7424 bp DNA SYN
DEFINITION pLenti X1 Puro empty (w610-1)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7424
/organism="pLenti X1 Puro empty (w610-1)"
/mol_type="other DNA"
promoter 62..90
/label="AmpR_promoter"
gene 132..992
/label="Ampicillin"
/gene="Ampicillin"
CDS 132..992
/label="ORF frame 3"
rep_origin 1147..1766
/label="pBR322_origin"
promoter 2075..2104
/label="lac_promoter"
misc_feature 2118..2140
/label="M13_pUC_rev_primer"
promoter 2139..2157
/label="M13_reverse_primer"
promoter 2174..2193
/label="T3_promoter"
misc_feature 2448..2628
/label="HIV-1_5_LTR"
misc_feature 2448..2628
/label="truncHIV-1_3_LTR"
misc_feature 2736..2780
/label="HIV-1_psi_pack"
CDS 3168..3668
/label="ORF frame 3"
misc_feature 3290..3523
/label="RRE"
CDS 3629..4189
/label="ORF frame 2"
misc_feature 4016..4031
/label="cPPT"
misc_feature 4161..4185
/label="attB1"
misc_feature 4251..4275
/label="attB2"
misc_feature 4308..4883
/label="WPRE"
CDS 4396..5025
/label="ORF frame 1"
misc_feature complement(4943..4966)
/label="MSCV_rev_primer"
CDS 5188..6027
/label="ORF frame 1"
misc_feature 5340..5359
/label="mPGK_F_primer"
CDS complement(5353..6078)
/label="ORF frame 3"
gene 5428..6027
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 6148..6163
/label="cPPT"
misc_feature 6148..6169
/label="U3PPT"
misc_feature 6218..6398
/label="HIV-1_5_LTR"
misc_feature 6218..6398
/label="truncHIV-1_3_LTR"
terminator 6473..6592
/label="SV40_PA_terminator"
misc_feature 6561..6580
/label="EBV_rev_primer"
rep_origin 6610..6687
/label="SV40_origin"
misc_feature 6672..6691
/label="SV40pro_F_primer"
promoter complement(6778..6796)
/label="T7_promoter"
promoter complement(6806..6822)
/label="M13_forward20_primer"
misc_feature 6815..6958
/label="lacZ_a"
misc_feature complement(6815..6837)
/label="M13_pUC_fwd_primer"
rep_origin 6984..7290
/label="f1_origin"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tTCCAGTGTG
4141 GTGGAATTCT GCAGATATCA ACAAGTTTGT ACAAAAAAGC AGGCTTTAAA GGAACCAATT
4201 CAGTCGACTG GATCCGGTAC CGAATTCGCG GCCGCACTCG AGATATCTAG ACCCAGCTTT
4261 CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC TCGAcaatca acctctggat
4321 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
4381 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
4441 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
4501 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
4561 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
4621 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
4681 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
4741 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
4801 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
4861 acgagtcgga tctccctttg ggccgcctcc ccgcctgGAA TTCTACCGGG TAGGGGAGGC
4921 GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA CTTGGCGCTA
4981 CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC AACCGGCTCC
5041 GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA AGTTCCCCCC
5101 CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA GTAGCACGTC TCACTAGTCT
5161 CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA GGCCTTTGGG GCAGCGGCCA
5221 ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG GGAAGGGGTG GGTCCGGGGG
5281 CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA AGGTCCTCCG GAGGCCCGGC
5341 ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC TCCTCTTCCT CATCTCCGGG
5401 CCTTTCGACC TGCAGCCCAA GCTTACCATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC
5461 ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC
5521 GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA
5581 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC
5641 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC
5701 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC
5761 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG
5821 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC
5881 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC
5941 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG
6001 TGCATGACCC GCAAGCCCGG TGCCTGACGC CCGCCCCACG ACCCGCAGCG CCCGACCGAA
6061 AGGAGCGCAC GACCCCATGC ATCTCGAGGG CCCGGTACct ttaagaccaa tgacttacaa
6121 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca
6181 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
6241 ctgagcctgg gagctctctg gctgcctagg gaacccactg cttaagcctc aataaagctt
6301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
6361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
6421 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
6481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6601 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
6661 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
6721 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct
6781 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
6841 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
6901 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
6961 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
7021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
7081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
7141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
7201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
7261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
7321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
7381 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcc
//
