| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBTB-3 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 3586 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBTB-3载体质粒基本信息
pBTB-3质粒图谱载体图谱和pBTB-3载体序列质粒序列多克隆位点信息
pBTB-3质粒载体简介
pBTB-3质粒序列载体序列
LOCUS DQ058731 3586 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBTB-3, complete sequence.
ACCESSION DQ058731
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..3586
/organism="Broad host range vector pBTB-3"
/mol_type="other DNA"
/db_xref="taxon:334963"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99723.1"
/db_xref="GI:69048329"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..2290
/gene="cat"
CDS 1631..2290
/gene="cat"
/codon_start=1
/transl_table=11
/product="chloramphenicol acetyl transferase"
/protein_id="AAY99724.1"
/db_xref="GI:69048330"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
regulatory 2309..2340
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(2368..3297)
/gene="araC"
CDS complement(2368..3297)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99725.1"
/db_xref="GI:69048331"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 3305..3572
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
1681 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
1741 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
1801 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
1861 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
1921 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
1981 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
2041 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
2101 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
2161 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
2221 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
2281 cggggcgtaa acgcgtggat ccccctcaag tcaaaagcct ccggtcggag gcttttgact
2341 ttctgctatg gaggtcaggt atgattttta tgacaacttg acggctacat cattcacttt
2401 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg
2461 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg
2521 ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac
2581 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg
2641 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca
2701 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
2761 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc
2821 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
2881 cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta
2941 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc
3001 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc
3061 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt
3121 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
3181 acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc
3241 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
3301 acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct
3361 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
3421 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
3481 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact
3541 ctctactgtt tctccatacc cgtttttttg ggctagagaa ttcgtc
//
