出品人: | Lynch,M.D. and Gill,R.T. |
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载体名称: | pBTB-4 |
质粒类型: | 广宿主载体;克隆载体;原核表达载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | araBAD |
载体大小: | 2936 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 四环素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 多个菌属 |
备注: | -- |
产品目录号: | -- |
组成型/诱导型: | 诱导型 |
买家导航
pBTB-4载体质粒基本信息
pBTB-4质粒图谱载体图谱和pBTB-4载体序列质粒序列多克隆位点信息
pBTB-4质粒载体简介
pBTB-4质粒序列载体序列
LOCUS DQ058722 2936 bp DNA circular SYN 08-MAY-2006 DEFINITION Broad host range vector pBT-4, complete sequence. AUTHORS Lynch,M.D. and Gill,R.T. TITLE Direct Submission JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering, University of Colorado, ECCH 111, Campus Box 424, Boulder, CO 80309, USA FEATURES Location/Qualifiers source 1..2936 /organism="Broad host range vector pBT-4" /mol_type="other DNA" /db_xref="taxon:334954" regulatory 60..96 /regulatory_class="terminator" /note="SoxR terminator" gene 872..1534 /gene="rep" /note="rep gene from pBBR1, GenBank Accession Number X66730" CDS 872..1534 /gene="rep" /note="required for replication" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAY99693.1" /db_xref="GI:69048133" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" gene 1631..2821 /gene="tetA" /note="tetracycline resistance gene from pBR322" CDS 1631..2821 /gene="tetA" /codon_start=1 /transl_table=11 /product="tetracycline/H+ antiporter" /protein_id="AAY99694.1" /db_xref="GI:69048134" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" regulatory 2841..2872 /regulatory_class="terminator" /note="TonB terminator" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg 121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca 181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc 241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag 301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc 361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc 421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg 481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt 541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg 601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg 661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac 721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg 781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa 841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat 901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg 961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca 1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg 1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt 1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg 1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca 1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta 1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat 1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg 1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga 1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca 1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa 1621 ggaagctaaa atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga 1681 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca 1741 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt 1801 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct 1861 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg 1921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg 1981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag 2041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat 2101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg 2161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt 2221 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 2281 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg 2341 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat 2401 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa 2461 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc 2521 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat 2581 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca 2641 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc 2701 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct 2761 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg 2821 aatggaaggg atccccctca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta 2881 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtc //