| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET42a, pET 42a, pET-42a |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5930 bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3' |
| 3' 测序引物及序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-GST, N-His, N-S, C-His, N-Thrombin, N-Factor Xa |
| 载体抗性: | 卡那 |
| 备注: |
pET42a,b,c的差异主要是在多克隆位点处。
|
| 产品目录号: | 70561-3 |
| 稳定性: | 瞬时表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET42a载体基本信息
pET42a载体图谱质粒图谱和多克隆位点信息
pET42a载体简介
pET42系列载体是用来进行克隆和高水平表达含有220个氨基酸的GST融合纯化标签的蛋白表达载体。 pET42a载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。 把表达的基因插入到载体的PshAI限制性内切酶位点时，可以通过Factor Xa蛋白酶将融合表达的GST标签完全切除。 
pET42a载体序列
LOCUS pET-42a(+) 5930 bp DNA Circular SYN
DEFINITION pET-42a(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5930
/organism="pET-42 a(+)"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(139..1092)
/label="ORF frame 3"
misc_feature complement(307..351)
/label="S15"
misc_feature complement(394..411)
/label="6xHIS"
gene complement(442..1092)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1093..1821
/label="ORF frame 1"
misc_feature complement(1101..1117)
/label="T7_transl_en_RBS"
misc_feature complement(1135..1162)
/label="lacO"
promoter complement(1162..1180)
/label="T7_promoter"
gene 1216..1479
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1268..1287)
/label="pBRrevBam_primer"
misc_feature 1562..2653
/label="lacI"
CDS 1694..2653
/label="ORF frame 2"
misc_feature 3225..3416
/label="ROP"
misc_feature complement(3410..3432)
/label="pGEX_3_primer"
rep_origin complement(3831..4450)
/label="pBR322_origin"
gene 4556..5371
/label="KanR2"
/gene="KanR2"
CDS 4556..5371
/label="ORF frame 2"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatcccatgg acccgcgtcc ctcaataccg gagccaccac cggtacccag
301 atctgggctg tccatgtgct ggcgttcgaa tttagcagca gcggtttctt tcataccaat
361 tgcagtacta ccgcgtggca ccagacccgc ggagtgatgg tgatggtgat gaccagaacc
421 actagttgaa ccatccgatt ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc
481 ctgcaaaggc catgctatat acttgctgga tttcaagtac ttatcaattt gtgggatagc
541 ttcaatacgt tttttaaaac aaactaattt tgggaacgca tccaggcaca ttgggtccat
601 gtataaaaca acatcaagag cgtcatacaa catgaagtca ggatgggtta catgatcacc
661 atttaaatat gttttatgac ataaacgatc ttcgaacatt ttcagcattt caggtagctt
721 gctaagaaaa tcaactttga gagtttcaaa gtctttacta tatgcaattc tcgaaacacc
781 gtatctaata tccaaaaccg ctccttcaag cattgaaatc tctgcacgct cttttggaca
841 accacccaac atgttgtgct tgtcagctat ataacgtatg atggccatag actgtgttaa
901 tttaacatca ccatcaatat aataaggaag attgggaaac tccaaaccca attcaaactt
961 tttgtttcgc catttatcac cttcatcgcg ctcatacaaa tgctcttcat atttttcttc
1021 aagatattcc aaaagaagtc gagtgggttg cacaaggccc ttaattttcc aataacctag
1081 tataggggac atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa
1141 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg
1201 atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
1261 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
1321 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
1381 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
1441 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat
1501 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
1561 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
1621 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
1681 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
1741 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
1801 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
1861 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
1921 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
1981 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
2041 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
2101 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
2161 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
2221 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
2281 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
2341 taccgagtcc gggctgcgcg ttggtgcgga catctcggta gtgggatacg acgataccga
2401 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
2461 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
2521 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
2581 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
2641 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat
2701 ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
2761 tgactagcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
2821 ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
2881 cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
2941 aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
3001 ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
3061 tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
3121 accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
3181 tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
3241 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
3301 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
3361 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
3421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
3481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
3541 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
3601 gcaccatata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
3661 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3721 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3781 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3841 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3901 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3961 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
4021 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
4081 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
4141 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
4201 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
4261 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
4321 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4381 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4441 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4501 ttggtcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
4561 ccatattcaa cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga
4621 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
4681 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
4741 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
4801 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
4861 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
4921 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
4981 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
5041 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
5101 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
5161 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
5221 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
5281 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
5341 gtttcatttg atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg
5401 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
5461 ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
5521 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
5581 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
5641 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
5701 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
5761 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
5821 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
5881 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//
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pET42a载体质粒应用举例
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