| 质粒名称: | pUF1-dCas9-GCN5 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 122509 |
| 存储实验室: | Lubin Jiang |
|
载体骨架基本信息 |
|
| 载体名称: | pUF1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10871 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | CRISPR |
| 筛选标记: | 囊虫素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-GCN5,dCas9-HAT |
| 物种来源: | 恶性疟原虫 |
| 插入大小: | -- |
| 基因突变: | dCas9(D10A,H840A) |
| GenBank编号 | -- |
| 启动子 | PfHsp86 |
| 标签/融合蛋白: | 3*标志（插入N端子） |
| 克隆方法: | 非连接克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | 未知 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pUF1-dCas9-GCN5 was a gift from Lubin Jiang (Addgene plasmid # 122509 ; http://n2t.net/addgene:122509 ; RRID:Addgene_122509) |
| 参考文献部分: | Epigenetic editing by CRISPR/dCas9 in Plasmodium falciparum. Xiao B, Yin S, Hu Y, Sun M, Wei J, Huang Z, Wen Y, Dai X, Chen H, Mu J, Cui L, Jiang L. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi: 10.1073/pnas.1813542116. Epub 2018 Dec 24. 10.1073/pnas.1813542116 PubMed 30584102 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUF1-dCas9-GCN5 10847 bp ds-DNA circular SYN 03-JUN-2019
DEFINITION Combining with the specific sgRNA sequence to activate of the
Plasmodium falciparum endogenous gene transcription, through the
increasing of H3K9 acetylation..
ACCESSION .
VERSION .
KEYWORDS pUF1-dCas9-GCN5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10847)
AUTHORS Xiao B, Yin S, Hu Y, Sun M, Wei J, Huang Z, Wen Y, Dai X, Chen H, Mu
J, Cui L, Jiang L
TITLE Epigenetic editing by CRISPR/dCas9 in Plasmodium falciparum.
JOURNAL Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):255-260. doi:
10.1073/pnas.1813542116. Epub 2018 Dec 24.
PUBMED 30584102
REFERENCE 2 (bases 1 to 10847)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 3, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10847
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(1540..1587)
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS complement(1588..5688)
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(5713..5733)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(5740..5805)
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 7298..7699
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MHAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
primer_bind complement(8407..8426)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8526..8548
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(8586..8604)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 8672..8776
/gene="bla"
/label=AmpR promoter
CDS 8777..9637
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8995..9014)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 9808..10396
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10297..10316
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 10550..10567
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 10684..10705
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10720..10750
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10758..10774
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10763..10785
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 10782..10798
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 10782..10798
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 10816..10834
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 10816..10833
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 ggccgcctac cctgaagaag aaaagtccga tgatgttgtg gacataaaga attgagctgt
61 ctcaaaaaat gagaaaacaa acaaacaaac aaaataccat tatgcgcata tcctctgctc
121 aatatcaatt tttctttatg ccagttttgt ttcgacacta tttgagcaac accttccctt
181 tactatctac tactgttaca atgttatttt attcaccttt ttacaatttt atcaaaattt
241 ttttgtatgc atataaatta tacattgtaa agccaaagtg tagataaaga acaccttgtt
301 tatttttccc caattttttt tttttaccat ttttttatta accttgctca tttacctgct
361 aatacgattg ctatatgata tcgaaattga aggaaaaaac atcatttgtg tttatatgtt
421 tttttttaat ttttcaactt tttttattta actatgctaa tcatattata aacattttaa
481 aaatcaagtc caactattta tgaatcattg aagagacaac ataattataa cagtaaaaat
541 aataataata ataataattt tgtcttattg tttcaaacat attatgttcc ataaatatct
601 aaatttttaa tatggtcata aattcatagg atttaattta tataaaatat ggctattcat
661 actagccatt ttatgtgtgt aaagtttttt ttttctgaac aaaaaataaa atgttcataa
721 ttttagctat ttacatgcat gtgcatgcac aaaaaaaaat atgcacacaa catacacatt
781 tttacagtta taaatacaat caattggtat aaatatataa ataagaaaaa cgaacattaa
841 gctgccatat ccttaattaa gtataaaatg tatagctttc tttactgctt ttttttgctc
901 atataacatt tccgaaagtc tcaaataatt gatgtttggg aaaatgtaac attccattaa
961 tgtaccacca tcataatctt taatatatcc aaaccatctt tcttttggca tggaaatttt
1021 ttgtgaaaat ccttgttttc taaaatatcc tatggcaaag ttatctgcat aggttaagaa
1081 atattctata ccaaacttct ttacatgctc cttcaaatga ttcattaatc ttgtaccata
1141 tccttttacc tgctcagttg aggtaacagc taaaaatgca atttccgcaa atttctgttc
1201 aaaatatggt ctgaaacata ctccacctat aacagtattt ttttttaata aacaaaatgt
1261 ataatgattt ctatcaaata caagccttac tatatattct ctaggcattt ttggtaactg
1321 tcttgagaaa atatttttca aagttattaa ttttattaaa tgatctggtt ccctatcgtt
1381 cgttatacat tcaaatgtta taatacccat attctcttct tttgctccac ccgcatctct
1441 gtgcaaatat cctaatcctg tttcggaggg tagtagagac tgtaacttat cgctctgctt
1501 cgtggcattt ataaacagca tgctgccgct gccagagccc tttttctttt ttgcctggcc
1561 ggcctttttc gtggccgccg gccttttgtc gcctcccagc tgagacaggt cgatccgtgt
1621 ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct
1681 ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag
1741 attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg
1801 gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt
1861 ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa
1921 cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag
1981 gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc
2041 ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag
2101 cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg
2161 attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt
2221 cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt
2281 ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt
2341 atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt
2401 ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg caaaatcccg
2461 gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag gccgcttccg
2521 gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt tgctgtagaa
2581 gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca tcttccgcac
2641 gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt actttttgat
2701 cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt agttgttgat
2761 ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct tggacttcag
2821 ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag tgttcatccg
2881 ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca gctgtctctt
2941 gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc
3001 gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt
3061 cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt
3121 cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga ttgcgtccac
3181 atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt
3241 ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc
3301 tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg
3361 gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt
3421 ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag
3481 gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc
3541 gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg
3601 gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt
3661 cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc
3721 ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt
3781 tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc
3841 ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag
3901 cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac
3961 ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt
4021 ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc tcaggaaggc
4081 gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat acacggtgaa
4141 gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt tcttatcgaa
4201 gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca cttcctcgaa
4261 gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc tgctgtttcc
4321 cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct cgatcttttc
4381 ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg cgtgcagctc
4441 tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct tccgcagcag
4501 gtcctctctg ttcagcttca cgagcagttc ctcggtgccg tccatctttt ccaggatggg
4561 cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc cggcgtagcc
4621 gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct gccgcacgag
4681 agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca tagaggcgct
4741 caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca ggatggcgtc
4801 ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct gggccagcag
4861 gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat cctcggccag
4921 gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca ggtttccgaa
4981 caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagcc gtctgctctt
5041 gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga tggggttttc
5101 ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca cgtcgctgtt
5161 gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca tgtgggccag
5221 ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt tctttctcag
5281 gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt tgccgaagat
5341 ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca gtctgtggaa
5401 gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg
5461 gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg cctcggctgt
5521 ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt gccggtcggt
5581 gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga tcacggccca
5641 gcccacagag ttggtgccga ttgccaggcc gatgctgtac ttcttgtcgg ctgctgggac
5701 tccgtggata ccgaccttcc gcttcttctt tggggccatc ttatcgtcat cgtctttgta
5761 atcaatatca tgatccttgt agtctccgtc gtggtcctta tagtccatct cgagtttatt
5821 cgaaatgtgg gaagaaaaaa aatataataa aaaaggaaaa tttcttttta aagtaaaata
5881 tttatgtaaa tatttatata ttttattata tatatattat tatatatata tatatatata
5941 tatatatacc aataaaataa tataaaaatt tttatgtata tctaaaaata tgtttttatt
6001 aaaaggtaaa aataaactta ttttttcttt ttttttaaaa aatatatata tatatatata
6061 tatataatat tatatgagat atatattaaa tattatatta tttatataaa tataattttt
6121 ttcttaattt ctcacgttgt taaaatttat atatttatat atgatatata ttttttaata
6181 ctttttaatt ttttttatta tttttggtaa ttatcttatt ttattttaat tttattattt
6241 tatattaaat gatgcttttt attttatatt attattttct atttaaaatt tttgctaaac
6301 atgaaattat tttattatat tttgtaaata caaaataaaa taatatataa atcaaaaaaa
6361 aaaaaagaaa gaaagaaaga aagatgatat ttaaaaaaaa aaaaagatat ttataaaaaa
6421 tataatatta tatataaaaa tattattttt atataaatat atttatatat ttatatatat
6481 gtatatatat atatatatat ataggtatag tatttattat tttgttatat ttatataata
6541 ctcctttttg ttattttttt tttttttttc tttaaattca tgcaaaaatt tactataata
6601 ttattatatt taactatata ctatggaata ctaaatatat atccaatggc ccctttccgc
6661 ggggaggact agtacgaaga tctgtcttaa gcttagctaa ttcgcttgta agaggtactc
6721 tcgtttatgc aaaactattt gatatagcat tttaacaagt acacatatat atatgtaata
6781 tatatactat atatatctat tgcatgtgta ctaagcatgt gcatggcatc ccctttttct
6841 cgtgtttaaa acagtttgta tgataaaata taaaggattt gaaaaagaga aaaaaatata
6901 tgatctcatc ctatatagcg ccataatttt tatttgggtt gaataaaatt ttctactaaa
6961 tttaggtgta agtaaaataa tggaatatat ataagtacaa taaaaaagtg cataaattaa
7021 aaaattttta taataaatat tttttttaaa aaagtcaata ataatattaa atatatataa
7081 cacaggatta tatatgttca ctacaatttt ttatattata atataaattc ttttcaattt
7141 tcattttatt ttacatacac tttccttttt tgtcactata ttttaatatt cacatattta
7201 gtttaaatac tggctatttc tttctacatt tgctagtaac aattgtgtag tgcttatata
7261 tatacacaca cctaaaactt acaaaccatg gtgataaatg catgccaagc ctttgtctca
7321 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
7381 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
7441 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
7501 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
7561 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
7621 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
7681 ttatgtgtgg gagggctaag gtaccccatt aaatttattt aataatagat taaaaatatt
7741 ataaaaataa aaacataaac acagaaatta caaaaaaaat acatatgaat ttttttttgt
7801 aatcttcctt ataaatatag aataatgaat catataaaac atatcattgt tcatttattt
7861 acatttaaaa ttattgtttc agtatcttta atttattatg tatatataaa aataacttac
7921 aattttatta ataaacaata tatgtttatt aattcatgtt ttgtaattta tgggatagcg
7981 atttttttta ctgtctgtat ttttcttttt taattatgtt ttaattgtat tttattttta
8041 ttattgttct ttttatagta ttattttaaa acaaaatgca ttttctaaga acttataata
8101 ataataaata taaattttaa taaaaattat atttatcttt tacaatatga acataaagta
8161 caacattaat atatagcttt taatattttt attcctaatc atgtaaatct taaatttttc
8221 tttttaaaca tatgttaaat atttatttct cattatatat aaggacatat ttattaaacc
8281 gcagagaaat ctagaggtac gaattcatat cgataactcc atggaactcc taggctggcg
8341 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
8401 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
8461 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
8521 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
8581 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
8641 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
8701 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
8761 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
8821 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
8881 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
8941 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
9001 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
9061 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
9121 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
9181 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
9241 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
9301 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
9361 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
9421 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
9481 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
9541 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
9601 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
9661 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
9721 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
9781 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
9841 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
9901 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
9961 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
10021 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
10081 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
10141 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
10201 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
10261 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
10321 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
10381 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
10441 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
10501 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
10561 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
10621 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
10681 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
10741 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
10801 tgattacgcc aagctattta ggtgacacta tagaatactc aagctgc
//
