pMKO.1-GFP

价格：1500元

联系方式：I47-825O-882O

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pMKO.1-GFP载体质粒基本信息

出品人:	爱迪基因
载体名称:	pMKO.1-GFP
质粒类型:	逆病毒载体；RNAi载体
高拷贝/低拷贝:	低拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	U6
载体大小:	6767 bp
5' 测序引物及序列:	pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3'
3' 测序引物及序列:	pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:	无
载体抗性:	氨苄青霉素
筛选标记:	EGFP
克隆菌株:	DH5α、HB101等
宿主细胞（系）:	常规哺乳动物细胞系
备注:	推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。
产品目录号:	#10676
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	逆转录病毒

pMKO.1-GFP质粒图谱载体图谱和pMKO.1-GFP载体序列质粒序列多克隆位点信息

pMKO.1-GFP载体图谱

pMKO.1-GFP质粒载体简介

pMKO.1-GFP质粒序列载体序列

LOCUS       pMKO.1-GFP Sequencing Result	6767 bp 	DNA	SYN
DEFINITION  pMKO.1 GFP Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="pMKO.1 GFP Sequencing Result"
                     /mol_type="other DNA"
     promoter        complement(232..434)
                     /label="SV40_promoter"
     promoter        complement(286..554)
                     /label="SV40_promoter"
     misc_feature    complement(306..325)
                     /label="SV40pro_F_primer"
     rep_origin      complement(310..387)
                     /label="SV40_origin"
     misc_feature    357..572
                     /label="SV40_enhancer"
     misc_feature    552..572
                     /label="pBABE_3_primer"
     misc_feature    708..730
                     /label="pGEX_3_primer"
     CDS             complement(1410..2129)
                     /label="ORF frame 1"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2134..2150
                     /label="pBluescriptKS_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2154..2356)
                     /label="SV40_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2208..2476)
                     /label="SV40_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2228..2247)
                     /label="SV40pro_F_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     rep_origin      complement(2232..2309)
                     /label="SV40_origin"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2279..2494
                     /label="SV40_enhancer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2474..2494
                     /label="pBABE_3_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2557..2813)
                     /label="hU6_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2608..2627)
                     /label="LKO1_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2834..3643)
                     /label="psi_plus_pack"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2837..3244)
                     /label="gag"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2850..2866)
                     /label="pBABE_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2882..2904)
                     /label="MSCV_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2882..2904)
                     /label="pLXSN_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(3713..4412)
                     /label="5_LTR"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(3891..4471)
                     /label="CMV_immearly_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(3918..3938)
                     /label="CMV_fwd_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(4104..4391)
                     /label="CAG_enhancer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        4693..4721
                     /label="AmpR_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        4903..4931
                     /label="AmpR_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     gene            4973..5833
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     CDS             4973..5833
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5988..6607
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ccatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcCTTTGAGT
   61 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
  121 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
  181 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TAAGCTTTTT
  241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA
  301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC
  361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA
  421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
  481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
  541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCCGG ATCTACACTC CGCTATCGCT
  601 ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
  661 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
  721 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCCAGCTT ACCTCCCGGT
  781 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG
  841 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA
  901 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC
  961 AATCGGAGGA CTGGCGCCCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC
 1021 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG
 1081 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGGG TCGACCCTGT
 1141 TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC
 1201 CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC
 1261 CCCCTTTTTC TGGAGACTAA ATAAAATCTT TTATTTTATC TATGGCTCGT ACTCTATAGG
 1321 CTTCAGCTGG TGATATTGTT GAGTCAAAAC TAGAGCCTGG ACCACTGATA TCCTGTCTTT
 1381 AACAAATTGG ACTAATCGAA GGTACCCTAT TACACCCACT CGTGCAGGCT GCCCAGGGGC
 1441 TTGCCCAGGC TGGTCAGCTG GGCGATGGCG GTCTCGTGCT GCTCCACGAA GCCGCCGTCC
 1501 TCCACGTAGG TCTTCTCCAG GCGGTGCTGG ATGAAGTGGT ACTCGGGGAA GTCCTTCACC
 1561 ACGCCCTTGC TCTTCATCAG GGTGCGCATG TGGCAGCTGT AGAACTTGCC GCTGTTCAGG
 1621 CGGTACACCA GGATCACCTG GCCCACCAGC ACGCCGTCGT TCATGTACAC CACCTCGAAG
 1681 CTGGGCTGCA GGCCGGTGAT GGTCTTCTTC ATCACGGGGC CGTCGTTGGG GAAGTTGCGG
 1741 CCCTTGTACT CCACGCGGTA CACGAACATC TCCTCGATCA GGTTGATGTC GCTGCGGATC
 1801 TCCACCAGGC CGCCGTCCTC GTAGCGCAGG GTGCGCTCGT ACACGAAGCC GGCGGGGAAG
 1861 CTCTGGATGA AGAAGTCGCT GATGTCCTCG GGGTACTTGG TGAAGGTGCG GTTGCCGTAC
 1921 TGGAAGGCGG GGCTCAGGAT GTCGAAGGCG AAGGGCAGGG GGGCGCCCTT GGTCACGCGG
 1981 ATCTGCACCA GCTGGTTGCC GAACAGGATG TTGCCCTTGC CGCAGCCCTC CATGGTGAAC
 2041 ACGTGGTTGT TCACCACGCC CTCCAGGTTC ACCTTGAAGC TCATGATCTC CTGCAGGCCG
 2101 GTGTTCTTCA GGATCTGCTT GCTCACCATG GTCTCGAGGT CGACGGTATC GATAAGCTTT
 2161 TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC
 2221 GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG
 2281 GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT
 2341 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA
 2401 CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT
 2461 GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGGG TCGACCACTG TGCTGGCGAA
 2521 TTCCATATGG GAGTACTAGT ACGCGTACCG GTGTTTCGTC CTTTCCACAA GATATATAAA
 2581 GCCAAGAAAT CGAAATACTT TCAAGTTACG GTAAGCATAT GATAGTCCAT TTTAAAACAT
 2641 AATTTTAAAA CTGCAAACTA CCCAAGAAAT TATTACTTTC TACGTCACGT ATTTTGTACT
 2701 AATATCTTTG TGTTTGCAGT CAAATTAATT CTAATTATCT CTCTAACAGC CTTGTATCGT
 2761 ATATGCAAAT ATGAAGGAAT CATGGGAAAT AGGCCCTCTT CCTGCCCGAC CTTGTCGACA
 2821 GATCTTCAAT TCCGGCGCCT AGAGAAGGAG TGAGGGCTGG ATAAAGGGAG GATCGAGGCG
 2881 GGGTCGAACG AGGAGGTTCA AGGGGGAGAG ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG
 2941 GCTTAGGGTG TACAAAGGGC TTGACCCAGG GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG
 3001 TCACGATGTA GGGGACCTGG TCTGGGTGTC CATGCGGGCC AGGTGAAAAG ACCTTGATCT
 3061 TAACCTGGGT GATGAGGTCT CGGTTAAAGG TGCCGTCTCG CGGCCATCCG ACGTTAAAGG
 3121 TTGGCCATTC TGCAGAGCAG AAGGTAACCC AACGTCTCTT CTTGACATCT ACCGACTGGT
 3181 TGTGAGCGAT CCGCTCGACA TCTTTCCAGT GACCTAAGGT CAAACTTAAG GGAGTGGTAA
 3241 CAGTCTGGCC CTAATTTTCA GACAAATACA GAAACACAGT CAGACAGAGA CAACACAGAA
 3301 CGATGCTGCA GCGCTGCAGC AGACAAGACG CGCGGCTTCG GTTCCAAACC GAAAGCAAAA
 3361 ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC ACATATCCTG
 3421 ACGGGGTCGG ATTCCACATC GACTCCCTTC CTCAGGTCGG GCCACAAAAA CGGCCCCCAA
 3481 AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC CGGGTGTTCA GAACTCGTCA GTTCCACCAC
 3541 GGGTCCGCCA GATACAGAGC TAGTTAGCTA ACTAGTACAG ACGCAGGCGC ATAACATCAA
 3601 ACATAGACAC TAGACAATCG GACAGACACA GATAAGTTGC TGGCCAGCTT ACCTCCCGGT
 3661 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCAAATCCCG GACGAGCCCC CAAATGAAAG
 3721 ACCCCCGTCG TGGGTAGTCA ATCACTCAGA GGAGACCCTC CCAAGGAACA GCGAGACCAC
 3781 GATTCGGATG CAAACAGCAA GAGGCTTTAT TGGGAATACG GGTACCCGGG CGACGCAGTC
 3841 TATCGGAAGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCTGCTTAT
 3901 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG
 3961 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG
 4021 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA
 4081 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA
 4141 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC
 4201 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG
 4261 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA
 4321 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC
 4381 CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA
 4441 TTACTATTAA TAACTAGTCA ATAATCAATG TCAACATGGC GGTAATGTTG GACATGAGCC
 4501 AATATAAATG TACATATTAT GATATGGATA CAACGTATGC AATGGCCAAG CTAATTCTTG
 4561 AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
 4621 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
 4681 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
 4741 ATAATTAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
 4801 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG
 4861 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
 4921 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
 4981 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
 5041 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
 5101 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
 5161 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
 5221 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
 5281 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
 5341 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
 5401 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
 5461 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
 5521 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
 5581 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
 5641 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
 5701 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
 5761 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
 5821 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
 5881 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
 5941 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
 6001 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
 6061 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
 6121 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
 6181 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
 6241 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
 6301 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
 6361 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
 6421 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
 6481 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
 6541 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
 6601 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
 6661 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
 6721 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAG
//

pMKO.1-GFP载体图谱质粒图谱pdf版和pMKO.1-GFP载体序列质粒序列等相关资料下载

pMKO.1-GFP质粒载体应用举例

上一篇：pBABE-puro-IRES-EGFP
下一篇：pMKO.1-puro

pdKeima-Red-S1	pCREj-2
pcDNA4/HisMax B	pREP4
pENTR11-Dual	pFLAG-CMV-3
Pgex-HRV3C	pHSG422
pSH65	pJSC73
pSC101	pcDNA5/FRT
pIC20H	pCGN978
pSUMO	pIJ702-del35-55
pKLAC1	pDsRed-Monomer
pET-12c(+)	pALEX a,b,c
pDEST26	pET-32 EK/LIC
pEF1/V5-HisA	pSET152
pTi	pHB-1
pHV23	pLVX-DD-ZsGreen1 Control
pcDNA5/FRT/V5-His-TOPO	pUCTag
pDC515	pRB373
pCRE-DD-AmCyan1	pPIC9k-His
pBAD18	pGL4.45[luc2P/ISRE/Hygro]
pMal-c2G	pGL4.41[luc2P/HSE/Hygro]
pTet-Splice	pPIC ZαGB
pSUPER.neo+gfp	pIκB-EGFP
pFLAG-CMV2	pBBR1MCS-2
pLexm	pLLP-OmpA
pHY15	pOM2
pACT-MyoD	pET-28a-BFP-C
pCL-Ampho	pFA1
pBT-4	pGL4.38[luc2P/p53 RE/Hygro]
ISceI-GR-RFP	pMal-c4X
pUG6	pVPack-GP
pMDLg/pRRE	pcDNA6.2-DsRed2-MCS1 miR
pOrf681_TA	pHS85
pNEO	pRSET-EGFP
pBluescript II KS(-)	pCas9D10A-GFP
pBad43	pMTD1.3xA
pWF1	pAD-SV40T
pLNCX2	pET-32a(+)
pCYM1	pET-21a(+)
pATPase_TA	pCH2
pNEO-ars	pBD-NF-kB
pCDH-CMV-MCS-EF1a-RFP+BSD	pMT-Bip-V5-HisA
pUC4-KISS	pGL4.72[hRlucCP]
c-Flag pcDNA3	PX543
pPIC9	pL-brevisGH3-MBP
pTP-4	pML21
pAcGFP1-N2	pcDNA3.1-GP130v1
EWD299	pSC101-EcoRImeth+
pCRE-MetLuc2-Reporter	pET-41a(+)
pProLabel-C	pcDNA3.1(+)/CAT
pAGO	pEF6/myc-His B
pAN7-1	pSP72

EC2_10_dCas9_VPR_HC_sgRNA	MSCV-(pBabe mcs)- human p210BCR-ABL-IRES
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pTRE Tight ArchT-GFP
800 pSG5L HA PTEN wt	pCMV-MMLVgag-3xNES-ABE8e
pT4-CMV-GFP	CKS2 gRNA (BRDN0001149143)
pMSCV-IRES-GFP II (pMIG II)	pcDNA3.4_7C11-1 heavy chain
pAAV-EF1a-FLEX-GTB	pEGFP-puro
pLentiV2-dCas9-VP64	pCAS9-mCherry empty
3XMEF2-luc	pAraGFPCDF
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	LeGO-G
pLenti X2 Puro DEST (w16-1)	Cbh_v5 AAV-CBE N-terminal
BECLIN-HA WT	pMSCV-hygro-STING
pEGFP-LC3	PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a
pYES-mtGFP	pNN9
pAAVS1-PDi-CRISPRn	EC1000
pAc-Neo-OVA	pAAV-EFS-CjCas9-eGFP-HIF1a
pSBtet-GP	pAc-sgRNA-Cas9
pTRKH2	pM1s3AsR_TS-C7
pJKR-L-tetR	pJSC131 - Bacterial expression plasmid f
FUmGW	pMonAID_nCas9-PmCDA_Hyg_ALS
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK	TAL3393
pCMV-hA3G-BE3	pET28a-At-TRL
pGGA008	pTEF:ATP
pDIRECT_23D	GST-HA-GFP11-N1
pLVX-M-puro	pBHt2
pNK9	pXAT2
VVT1	pLenti-DsRed_IRES_MAPT:EGFP
PB_tre_Cas9	pM-Cas9
pNEU-hMbPylRS-4xU6M15	pLJC5-3XHA-EGFP-PEX26
1306 pSG5L HA FKHR wt	EC2_3_dCas9_Mxi1_sgRNA
phage UbiC NLS HA stdMCP stdHalo	pSR47
AbVec1.1-IGKC	pCS2TAL3-RR
pAAVS1-PC-GCaMP6f	NM9-SpCas9-NLS3
ilux pGEX(-)	pCDH-puro-Bcl-XL
p201G Cas9	pRC-CMV-Rev1b
pAC1404-pCR8-mRuby2_NLSPUFa	pMal-T-Avi-His/BirA
pICH86966::AtU6p::sgRNA_PDS	pC003 - LshC2C2 locus into pACYC184 for
LB1366 (Bcl-2 promoter with MHS1234)	c-myc-PT3EF1a
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I	pRAD1
pCXLE-hOCT3/4	pGL3 2 kb prom. CD274
p58sheLL	pUCC001
pPPEM	pMJ329: pCMV-MmeFz2
401 pSG5L	pSELECT-HA-mFOXO1
pCS2TAL3-DD	pHAGE-PTCH1
pCW-Cas9	pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
BPK3082	pFC334
pNeae2	pT3-Neo-EF1a-GFP
Lenti-luciferase-P2A-Neo	pMXs-hOCT4 (Plath)
pCAG-GBP1-10gly-lexADBD	pLenti-EML4-ALK variant 3a
Tet-O-FUW-Neurod1	pAAV2/8
pGH335_MS2-AID*Δ-Hygro	pQUAST>stop>mCD8-GFP

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