| 质粒名称: | pCMV-VSV-G-HiBiT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 162594 |
| 存储实验室: | Masaharu Somiya |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6540bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV-G |
| 物种来源: | vesicular stomatitis virus |
| 插入大小: | 1569bp |
| 启动子: | CMV |
| 标签/融合蛋白: | HiBiT (C terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | N/A |
| 3’测序引物 | Bglob-pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-VSV-G-HiBiT was a gift from Masaharu Somiya (Addgene plasmid # 162594 ; http://n2t.net/addgene:162594 ; RRID:Addgene_162594) |
买家导航
pCMV-VSV-G-HiBiT载体质粒基本信息
pCMV-VSV-G-HiBiT质粒图谱载体图谱和pCMV-VSV-G-HiBiT载体序列质粒序列多克隆位点信息
pCMV-VSV-G-HiBiT质粒载体简介
pCMV-VSV-G-HiBiT质粒序列载体序列
LOCUS pCMV-VSV-G-HiBiT 6540 bp DNA circular SYN 26-AUG-2024
DEFINITION C-terminally HiBiT-tagged VSV-G under CMV promoter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6540)
AUTHORS Somiya M, Kuroda S
TITLE Real-Time Luminescence Assay for Cytoplasmic Cargo Delivery of
Extracellular Vesicles.
JOURNAL Anal Chem. 2021 Mar 24. doi: 10.1021/acs.analchem.1c00339.
PUBMED 33759512
REFERENCE 2 (bases 1 to 6540)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Anal Chem.
2021 Mar 24. doi: 10.1021/acs.analchem.1c00339."
FEATURES Location/Qualifiers
source 1..6540
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 581..605
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 696..1268
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
CDS 1354..2886
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
primer_bind complement(3149..3168)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3214..3269
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3268..3287)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3627..3643)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3627..3643)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3640..3662)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3651..3667
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3675..3705)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3720..3741
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3858..3875)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4029..4617)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4109..4128)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4788..5648)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5411..5430
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5649..5753)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5779..6234)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5916..5937)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6128..6147
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6361..6383
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6375..6392
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6376..6392
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6402..6421
/label=T7
/note="T7 promoter, forward primer"
promoter 6402..6420
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
1321 tcctcgacgg atccctcgag gaattctgac actatgaagt gccttttgta cttagccttt
1381 ttattcattg gggtgaattg caagttcacc atagtttttc cacacaacca aaaaggaaac
1441 tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa gctcagattt aaattggcat
1501 aatgacttaa taggcacagc cttacaagtc aaaatgccca agagtcacaa ggctattcaa
1561 gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta cttgtgattt ccgctggtat
1621 ggaccgaagt atataacaca ttccatccga tccttcactc catctgtaga acaatgcaag
1681 gaaagcattg aacaaacgaa acaaggaact tggctgaatc caggcttccc tcctcaaagt
1741 tgtggatatg caactgtgac ggatgccgaa gcagtgattg tccaggtgac tcctcaccat
1801 gtgctggttg atgaatacac aggagaatgg gttgattcac agttcatcaa cggaaaatgc
1861 agcaattaca tatgccccac tgtccataac tctacaacct ggcattctga ctataaggtc
1921 aaagggctat gtgattctaa cctcatttcc atggacatca ccttcttctc agaggacgga
1981 gagctatcat ccctgggaaa ggagggcaca gggttcagaa gtaactactt tgcttatgaa
2041 actggaggca aggcctgcaa aatgcaatac tgcaagcatt ggggagtcag actcccatca
2101 ggtgtctggt tcgagatggc tgataaggat ctctttgctg cagccagatt ccctgaatgc
2161 ccagaagggt caagtatctc tgctccatct cagacctcag tggatgtaag tctaattcag
2221 gacgttgaga ggatcttgga ttattccctc tgccaagaaa cctggagcaa aatcagagcg
2281 ggtcttccaa tctctccagt ggatctcagc tatcttgctc ctaaaaaccc aggaaccggt
2341 cctgctttca ccataatcaa tggtacccta aaatactttg agaccagata catcagagtc
2401 gatattgctg ctccaatcct ctcaagaatg gtcggaatga tcagtggaac taccacagaa
2461 agggaactgt gggatgactg ggcaccatat gaagacgtgg aaattggacc caatggagtt
2521 ctgaggacca gttcaggata taagtttcct ttatacatga ttggacatgg tatgttggac
2581 tccgatcttc atcttagctc aaaggctcag gtgttcgaac atcctcacat tcaagacgct
2641 gcttcgcaac ttcctgatga tgagagttta ttttttggtg atactgggct atccaaaaat
2701 ccaatcgagc ttgtagaagg ttggttcagt agttggaaaa gctctattgc ctcttttttc
2761 tttatcatag ggttaatcat tggactattc ttggttctcc gagttggtat ccatctttgc
2821 attaaattaa agcacaccaa gaaaagacag atttatacag acatagagat gaaccgactt
2881 ggaaaggtga gcggctggcg gctgttcaag aagattagct aactcaaatc ctgcacaaca
2941 gattcttcat gtttggacca aatcaacttg tgataccatg ctcaaagagg cctcaattat
3001 atttgagttt ttaattttta tgaaaaaaaa aaaaaaaaac ggaattcctc gagggatccg
3061 tcgaggaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
3121 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
3181 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
3241 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
3301 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
3361 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
3421 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
3481 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
3541 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
3601 acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
3661 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
3721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
3781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
3841 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
3901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
4201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
4441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
4561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
4621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
4681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
4741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
4801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
4861 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
4921 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
4981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
5041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
5101 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
5161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
5221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
5281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
5341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
5401 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
5461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
5521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
5581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
5641 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
5701 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
5761 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
5821 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
5881 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
5941 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
6001 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
6061 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
6121 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
6181 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
6241 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
6301 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
6361 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
6421 gcgaattgga gctccaccgc ggtggcggcc gctctagaga gcttggccca ttgcatacgt
6481 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
//
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