| 出品公司: | -- |
|---|---|
| 载体名称: | pBBR1MCS-5, pBBR1MCS5, pBBRMCS5 |
| 质粒类型: | 广宿主蛋白表达或克隆载体质粒，广宿主载体质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 4.7 kb |
| 5' 测序引物及序列: | M13-F: TGTAAAACGACGGCCAGT |
| 3' 测序引物及序列: | M13-R: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 庆大霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多种微生物物种，是广宿主质粒。 |
| 备注: |
参考文献： [1] Kovach ME, Phillips RW, Elzer PH, Roop RM, 2nd, Peterson KM. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 1994;16:800-2. [2] Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, 2nd, et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995;166:175-6. GenBank sequence：XXU25061 |
| 产品目录号: | -- |
| 稳定性/瞬表达: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBBR1MCS-5载体pBBRMCS5质粒基本信息
pBBR1MCS-5质粒图谱pBBRMCS5载体图谱和pBBR1MCS-5载体序列pBBRMCS5质粒序列多克隆位点信息
pBBR1MCS-5质粒pBBRMCS5载体简介
pBBR1MCS-5质粒序列pBBRMCS5载体序列
LOCUS pBBR1MCS-5 4768 bp DNA linear SYN 10-JAN-2005
DEFINITION Cloning vector pBBR1MCS-5 REP, LacZ alpha peptide (lacZ alpha), and
gentamicin-3-acetyltransferase genes, complete cds.
ACCESSION U25061
VERSION U25061.1 GI:833825
KEYWORDS .
SOURCE Cloning vector pBBR1MCS-5
ORGANISM Cloning vector pBBR1MCS-5
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1 (bases 1 to 4768)
AUTHORS Kovach,M.E., Phillips,R.W., Elzer,P.H., Roop,R.M. II and
Peterson,K.M.
TITLE pBBR1MCS: a broad-host-range cloning vector
JOURNAL BioTechniques 16 (5), 800-802 (1994)
PUBMED 8068328
REFERENCE 2 (bases 1 to 4768)
AUTHORS Kovach,M.E., Elzer,P.H., Hill,D.S., Robertson,G.T., Farris,M.A.,
Roop,R.M. II and Peterson,K.M.
TITLE Four new derivatives of the broad-host-range cloning vector
pBBR1MCS, carrying different antibiotic-resistance cassettes
JOURNAL Gene 166 (1), 175-176 (1995)
PUBMED 8529885
REFERENCE 3 (bases 1 to 4768)
AUTHORS Kovach,M.E.
TITLE Direct Submission
JOURNAL Submitted (13-APR-1995) Michael E. Kovach, Microbiology, Louisiana
State University, 1501 Kings Highway, Shreveport, LA 71130, USA
FEATURES Location/Qualifiers
source 1..4768
/organism="Cloning vector pBBR1MCS-5"
/mol_type="genomic DNA"
/db_xref="taxon:51200"
/lab_host="Bordetella bronchiseptica"
CDS 1793..2455
/note="required for plasmid replication"
/codon_start=1
/transl_table=11
/product="REP"
/protein_id="AAB06694.1"
/db_xref="GI:833826"
gene complement(3013..3378)
/gene="lacZ alpha"
CDS complement(3013..3378)
/gene="lacZ alpha"
/codon_start=1
/transl_table=11
/product="LacZ alpha peptide"
/protein_id="AAB06695.1"
/db_xref="GI:833827"
CDS 3975..4508
/function="resistance to gentamicin"
/codon_start=1
/transl_table=11
/product="gentamicin-3-acetyltransferase"
/protein_id="AAB06696.1"
/db_xref="GI:833828"
ORIGIN
1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
3601 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
3661 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
3721 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
3781 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
3841 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
3901 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
3961 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
4021 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
4081 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
4141 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
4201 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
4261 tgcccaggtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggcg
4321 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
4381 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
4441 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
4501 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
4561 tgtcacaacg ccgccaggtg gcacttttcg gggaaatgtg cgcgcccgcg ttcctgctgg
4621 cgctgggcct gtttctggcg ctggacttcc cgctgttccg tcagcagctt ttcgcccacg
4681 gccttgatga tcgcggcggc cttggcctgc atatcccgat tcaacggccc cagggcgtcc
4741 agaacgggct tcaggcgctc ccgaaggt
//
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