| 质粒名称: | BB3cH_pGAP_23*_pLAT1_Cas9 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 104907 |
| 存储实验室: | Brigitte Gasser |
|
载体骨架基本信息 |
|
| 载体名称: | Episomal ARS/CEN Backbone |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 9323 bp |
| 载体类型: | Yeast Expression, CRISPR |
| 筛选标记: | Hygromycin |
| 载体抗性: | Hygromycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HH - FS23 linker - HDV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BbsI (not destroyed) |
| 3’克隆位点: | BbsI (not destroyed) |
| 5’测序引物 | GAP fw |
| 插入基因名称: | human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) / hcas9 |
| 物种来源： | H. sapiens (human); Streptococcus pyogenes, Pichia pastoris |
| 插入大小： | 4140bp |
|
文献引用方法 |
|
| 材料和方法部分: | BB3cH_pGAP_23*_pLAT1_Cas9 was a gift from Brigitte Gasser (Addgene plasmid # 104907 ; http://n2t.net/addgene:104907 ; RRID:Addgene_104907) |
| 参考文献部分: | CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris. Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R. Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9. 10.1007/978-1-4939-9024-5_9 PubMed 30737742 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BB3cH_pGAP_23*_pLAT1_Cas9 9323 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR
products for episomal expression in P. pastoris and selection on
Hyg.
ACCESSION .
VERSION .
KEYWORDS BB3cH_pGAP_23*_pLAT1_Cas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9323)
AUTHORS Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R.
TITLE CRISPR/Cas9-mediated homology directed genome editing in Pichia
pastoris
JOURNAL (2018) In Press
REFERENCE 2 (bases 1 to 9323)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9323
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(62..650)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(142..161)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 737..1213
/label=GAP promoter
/note="promoter for Pichia pastoris
glyceraldehyde-3-phosphate dehydrogenase"
CDS 2757..6860
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6873..6893
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 6965..7212
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6981..6999)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
gene 7232..8807
/label=hphMX6
/note="yeast selectable marker conferring hygromycin
resistance"
promoter 7232..7575
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 7576..8604
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
KE"
terminator 8610..8807
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
misc_feature 8819..9322
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
ORIGIN
1 cgctgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
661 tgatcttttc tacggggtgg agcgcaaaaa accccgcccc tgacagggcg gggttttttc
721 gcgatcggag gatccttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca
781 tctctgaaat atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc
841 cggggtaaaa cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct
901 tccaccgccc gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc
961 cccttgcagc aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg
1021 acctagcagc ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg
1081 catgtcatga gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca
1141 attttggttt ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca
1201 attgaacaac tatcaaaaca ccatggcgtc ttcggatccc tcgaggaaga cgcgctttta
1261 gtgtacatct gataatatag tactaccacg tatgataatg tagagaatag gcttccttgt
1321 cgagtgtgtt tgcagttttc ttgagtttca aggtttaaat gctggtatat tagttcatcg
1381 aaggtttcag ccaatagcac cttaaatcaa tcaaactaat tcgactctta cgaaagagcc
1441 tactgtgttt agtatcgaag tcgtttacct ttcatgttga atagcttcct ctctgaccct
1501 aacatttcaa gatcctccta aagttacccg gattgtgaaa ttctaatgat ccacctgccc
1561 aatgcatttt ttctttattc agtttacctt ttttacctaa tatacgagct tgttaaagta
1621 agtggcactg caatactagg cttattgttg atattatgat gaatcgtttt cacaaacttg
1681 atttcctgtg aactcaccat gtactaagga aaaaaacatg catcaccatc tgaatatttg
1741 accgctccgg ggagggaaga atatgaacca cgattgaaag actttgggtt tgccgattct
1801 aaaacactta ctgaacagaa aagaagtgaa ttattagaga gtttatgcaa cggagatcat
1861 gagcttagaa agaacgtagg ctgggcaaca acatccattt ctgcctgcga tatttccagt
1921 gcaatgctaa agccacataa tataggcaat tacaacttga atgagcaggc tcatgatgtc
1981 accatggaac tgatagataa agtcatccaa aaaggtgtca atttacggca cgtctacgta
2041 gatactgtcg gacctccagc atcataccaa aagaaactgc aacaaaggtt tcccgcactg
2101 ctgatcaccg tatctaaaaa agcagatgcc atctatccat gtgtttcaag tgcttcagtt
2161 tgcgccaagg tcactagaga cgtctgtttg agaacctcaa atggggagga gaacctatta
2221 gattgtgggt ctggatatcc ctcggaccca aatactaaga aatggctagc ccagtctata
2281 catccattgt ttggctggag tcgtgatgtc aggttttcgt ggcagacggc caaagattgt
2341 cttgagagac acaactcaat caaagttgtg tttggcgaag aggagcctac cccggttaat
2401 ttcggtgatc tggggttgag caaacagaag cccgacttta aggatttaga aatcagtacc
2461 agttggtggg gacattaatt ggtggaggag agccaatatt ctcaactgtt cgtaggacca
2521 taacccccca ctcgcaccag aaatactcat tggcgctgac aactagtttg agccgcttca
2581 attgcggcaa gtccccagtt ttgtttggta ctcccctctc gcctcgcgtg tttttgttcc
2641 tccgaattgt gcatcaccct gtcacatccg tacaccatcg gaacactctg ctcctcctcc
2701 tttctcctct taattgaacg tatatctaat taccctaata acaagtatct tcaaccatgg
2761 acaagaagta ctccattggg ctcgatatcg gcacaaacag cgtcggttgg gccgtcatta
2821 cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca
2881 gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca
2941 cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc
3001 tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg
3061 aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata
3121 tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc
3181 ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga
3241 tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg
3301 acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca
3361 acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc
3421 tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta
3481 tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg
3541 ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga
3601 tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc
3661 tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga
3721 tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt gtcagacagc
3781 aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc tacgccggat
3841 acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc atcttggaaa
3901 aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg ttgcgcaaac
3961 agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa ctgcacgcta
4021 tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa aagattgaga
4081 aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga aattccagat
4141 tcgcgtggat gactcgcaaa tcagaagaga ctatcactcc ctggaacttc gaggaagtcg
4201 tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt gataaaaatc
4261 tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc acagtttata
4321 acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca ttcctgtctg
4381 gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa gttaccgtga
4441 aacagctcaa agaggactat ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg
4501 gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg aaaatcatta
4561 aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac attgtcctca
4621 cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact tacgctcatc
4681 tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga tgggggcggc
4741 tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca atcctggatt
4801 ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat gatgactctc
4861 tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac agccttcacg
4921 agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg cagaccgtta
4981 aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat atcgttatcg
5041 agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg gaaaggatga
5101 agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa cacccagttg
5161 aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac ggcagggaca
5221 tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg gatcatatcg
5281 tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca agatccgata
5341 aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt
5401 attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat aatctgacta
5461 aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa aggcagcttg
5521 ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc atgaacacca
5581 agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg aagtctaagc
5641 tcgtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc aacaattacc
5701 accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc
5761 ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt aggaaaatga
5821 tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt tacagcaata
5881 ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg aagcgaccac
5941 ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg gatttcgcga
6001 cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc gaagtacaga
6061 ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac
6121 gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca gtcgcttaca
6181 gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg
6241 aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc atcgactttc
6301 tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt cccaagtact
6361 ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga
6421 aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg gccagccact
6481 atgaaaagct caaagggtcc cccgaagata atgagcagaa gcagctgttc gtggaacaac
6541 acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa agagtgatcc
6601 tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg gataagccca
6661 tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg ggcgcgcctg
6721 cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct acaaaggagg
6781 tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca agaatcgacc
6841 tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag gtgtgagctt
6901 cgtccgacgg cggcccgacg ggtccgaggc ctcggagatc cgtccccctt ttcctttgtc
6961 gatatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
7021 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
7081 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
7141 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
7201 gctttaattt gcaagctggc gctaattcat ggacatggag gcccagaata ccctccttga
7261 cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat
7321 acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa
7381 aaattacggc tcctcgctgc agacgtgcga gcagggaaac gctcccctca cagacgcgtt
7441 gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag
7501 gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc
7561 cgaacataaa caaccatggg taaaaagcct gaactcaccg cgacgtctgt cgagaagttt
7621 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
7681 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
7741 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
7801 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
7861 gcacagggtg tcacgttgca agacctccct gaaaccgaac tgcccgctgt tctgcagccg
7921 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
7981 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
8041 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
8101 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
8161 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
8221 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
8281 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
8341 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
8401 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
8461 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
8521 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
8581 actcgtccga gggcaaagga ataatcagta ctgacaataa aaagattctt gttttcaaga
8641 acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg
8701 tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga
8761 aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactggct tccttttcat
8821 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
8881 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
8941 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
9001 atgccgaatt gtttcatctt gtctgtgtag aacaccacac acgaaaatcc tgtgatttta
9061 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
9121 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
9181 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
9241 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
9301 tgtaagttac aggcaagcga tcg
//
