| 出品公司: | Invitrogen/ Ambion |
|---|---|
| 载体名称: | pSilencer5.1-U6 Retro, pSilencer 5.1-U6 Retro, pSilencer5.1-U6-Retro |
| 质粒类型: | 哺乳动物细胞逆病毒基因沉默载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | U6 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6486bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 备注: | 逆病毒基因沉默载体，能够将发卡的siRNA转染进入大多数哺乳细胞中。 |
| 产品目录号: | AM5784 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
pSilencer5.1-U6 Retro载体质粒基本信息
pSilencer5.1-U6 Retro质粒图谱载体图谱和pSilencer5.1-U6 Retro载体序列质粒序列多克隆位点信息
pSilencer5.1-U6 Retro质粒载体简介
pSilencer5.1-U6 Retro质粒序列载体序列
LOCUS pSilencer\5.1-U6 Retro 6486 bp DNA circular 6-OCT-2013
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pSilencer 5.1-U6 Retro|
BASE COUNT 1460 a 1840 c 1693 g 1493 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc
421 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
481 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
541 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt
601 ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca
661 ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca
721 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
781 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
841 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
901 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
961 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
1021 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
1081 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
1141 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg ctgatccttg
1201 ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt ggaggttcca
1261 ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag gtaagctggc
1321 cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt
1381 gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac
1441 tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg
1501 tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt
1561 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
1621 ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg
1681 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
1741 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
1801 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
1861 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
1921 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
1981 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
2041 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
2101 ctccctttat ccagccctca ctccttctct aggcgccgga attagatcga tctctcgagg
2161 tcgacggtat cgataagctt ttccaaaaaa gtactgctta cgatacggtc tcttgaaccg
2221 tatcgtaagc agtactggat cccgcgtcct ttccacaaga tatataaacc caagaaatcg
2281 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
2341 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
2401 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
2461 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg
2521 gtcacgctcc gtcacgtggt gcgttttgcc tgcgcgtctt tccactgggg aattctaccg
2581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
2641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
2701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
2761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
2821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
2881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
2941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
3001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
3061 ctcatctccg ggcctttcga cctgcagccc aagctagctt accatgaccg agtacaagcc
3121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
3181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
3241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
3301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
3361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
3421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
3481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
3541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
3601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
3661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
3721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa agattttatt
3781 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagagaa
3841 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
3901 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
3961 gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc
4021 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
4081 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc
4141 ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa
4201 ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc
4261 gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca cacaacatac
4321 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
4381 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
4441 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
4501 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4561 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4621 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4681 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4741 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4801 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4861 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4921 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4981 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
5041 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
5101 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
5161 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
5221 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
5281 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
5341 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
6181 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
6241 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
6301 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
6361 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
6421 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
6481 ttcgtc
//
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