LOCUS HA-HA-AR-DBD 5732 bp ds-DNA circular SYN 25-JUN-2021
DEFINITION Mammalian expression of 2xHA-tagged human androgen receptor
trancate: AR DNA-bindind domain (DBD).
ACCESSION .
VERSION .
KEYWORDS HA-HA-AR-DBD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5732)
AUTHORS Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu
J, Balk SP, Chen S
TITLE Protein phosphatase 1 suppresses androgen receptor ubiquitylation
and degradation.
JOURNAL Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
PUBMED 26636645
REFERENCE 2 (bases 1 to 5732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 25, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5732
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 928..954
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 970..996
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(1323..1340)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1329..1553
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1599..2027
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1686..1705)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1896..1917
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2036..2056)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2041..2370
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2221..2356
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2283..2302
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2437..3231
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2491..2510)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3101..3120
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3405..3526
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3442..3461)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3496..3515
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3575..3591)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3575..3591)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3588..3610)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3599..3615
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3623..3653)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3668..3689
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3806..3823)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3977..4565)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4057..4076)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4736..5596)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5359..5378
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5597..5701)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttccac catggcctac ccatacgacg tcccagacta cgctcagctc
961 cagcttatgt acccctacga cgtgcccgac tacgcggatc caatgcgttt ggagactgcc
1021 agggaccatg ttttgcccat tgactattac tttccacccc agaagacctg cctgatctgt
1081 ggagatgaag cttctgggtg tcactatgga gctctcacat gtggaagctg caaggtcttc
1141 ttcaaaagag ccgctgaagg gaaacagaag tacctgtgcg ccagcagaaa tgattgcact
1201 attgataaat tccgaaggaa aaattgtcca tcttgtcgtc ttcggaaatg ttatgaagca
1261 gggatgactc tgggatgagc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
1321 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
1381 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
1441 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
1501 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
1561 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
1621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
1681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
1741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
1801 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
1861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
1921 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
1981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
2041 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2101 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
2161 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2221 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2281 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
2341 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
2401 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
2461 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
2521 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
2581 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
2641 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
2701 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
2761 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
2821 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
2881 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
2941 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
3001 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
3061 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
3121 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
3181 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
3241 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
3301 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
3361 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
3421 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
3481 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
3541 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
3601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
3661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
3721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
3781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
3901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
3961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
4141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
4201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
4261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
4321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
4381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
4441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
4681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
4741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
4801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
4861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
4921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
4981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
5281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
5341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
5401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
5461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
5521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
5581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
5641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
5701 gcacatttcc ccgaaaagtg ccacctgacg tc
//
