LOCUS HA-HA-AR-NTD-DBD 7340 bp ds-DNA circular SYN 25-JUN-2021
DEFINITION Mammalian expression of 2xHA-tagged human androgen receptor
trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD).
ACCESSION .
VERSION .
KEYWORDS HA-HA-AR-NTD-DBD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7340)
AUTHORS Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu
J, Balk SP, Chen S
TITLE Protein phosphatase 1 suppresses androgen receptor ubiquitylation
and degradation.
JOURNAL Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
PUBMED 26636645
REFERENCE 2 (bases 1 to 7340)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 25, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7340
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 928..954
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 970..996
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(2931..2948)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2937..3161
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3207..3635
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3294..3313)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3504..3525
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3644..3664)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3649..3978
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3829..3964
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3891..3910
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4045..4839
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4099..4118)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4709..4728
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5013..5134
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5050..5069)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5104..5123
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5183..5199)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5183..5199)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5196..5218)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5207..5223
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5231..5261)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5276..5297
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5414..5431)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5585..6173)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5665..5684)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6344..7204)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6967..6986
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7205..7309)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttccac catggcctac ccatacgacg tcccagacta cgctcagctc
961 cagcttatgt acccctacga cgtgcccgac tacgcggatc caatggaagt gcagttaggg
1021 ctgggaaggg tctaccctcg gccgccgtcc aagacctacc gaggagcttt ccagaatctg
1081 ttccagagcg tgcgcgaagt gatccagaac ccgggcccca ggcacccaga ggccgcgagc
1141 gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca gcagcagcag
1201 cagcagcagc agcagcagca gcagcagcag cagcagcaag agactagccc caggcagcag
1261 cagcagcagc agggtgagga tggttctccc caagcccatc gtagaggccc cacaggctac
1321 ctggtcctgg atgaggaaca gcaaccttca cagccgcagt cggccctgga gtgccacccc
1381 gagagaggtt gcgtcccaga gcctggagcc gccgtggccg ccagcaaggg gctgccgcag
1441 cagctgccag cacctccgga cgaggatgac tcagctgccc catccacgtt gtccctgctg
1501 ggccccactt tccccggctt aagcagctgc tccgctgacc ttaaagacat cctgagcgag
1561 gccagcacca tgcaactcct tcagcaacag cagcaggaag cagtatccga aggcagcagc
1621 agcgggagag cgagggaggc ctcgggggct cccacttcct ccaaggacaa ttacttaggg
1681 ggcacttcga ccatttctga caacgccaag gagttgtgta aggcagtgtc ggtgtccatg
1741 ggcctgggtg tggaggcgtt ggagcatctg agtccagggg aacagcttcg gggggattgc
1801 atgtacgccc cacttttggg agttccaccc gctgtgcgtc ccactccttg tgccccattg
1861 gccgaatgca aaggttctct gctagacgac agcgcaggca agagcactga agatactgct
1921 gagtattccc ctttcaaggg aggttacacc aaagggctag aaggcgagag cctaggctgc
1981 tctggcagcg ctgcagcagg gagctccggg acacttgaac tgccgtctac cctgtctctc
2041 tacaagtccg gagcactgga cgaggcagct gcgtaccaga gtcgcgacta ctacaacttt
2101 ccactggctc tggccggacc gccgccccct ccgccgcctc cccatcccca cgctcgcatc
2161 aagctggaga acccgctgga ctacggcagc gcctgggcgg ctgcggcggc gcagtgccgc
2221 tatggggacc tggcgagcct gcatggcgcg ggtgcagcgg gacccggttc tgggtcaccc
2281 tcagccgccg cttcctcatc ctggcacact ctcttcacag ccgaagaagg ccagttgtat
2341 ggaccgtgtg gtggtggtgg gggtggtggc ggcggcggcg gcggcggcgg cggcggcggc
2401 ggcggcggcg gcggcggcga ggcgggagct gtagccccct acggctacac tcggccccct
2461 caggggctgg cgggccagga aagcgacttc accgcacctg atgtgtggta ccctggcggc
2521 atggtgagca gagtgcccta tcccagtccc acttgtgtca aaagcgaaat gggcccctgg
2581 atggatagct actccggacc ttacggggac atgcgtttgg agactgccag ggaccatgtt
2641 ttgcccattg actattactt tccaccccag aagacctgcc tgatctgtgg agatgaagct
2701 tctgggtgtc actatggagc tctcacatgt ggaagctgca aggtcttctt caaaagagcc
2761 gctgaaggga aacagaagta cctgtgcgcc agcagaaatg attgcactat tgataaattc
2821 cgaaggaaaa attgtccatc ttgtcgtctt cggaaatgtt atgaagcagg gatgactctg
2881 ggatgagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
2941 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
3001 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
3061 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
3121 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
3181 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
3241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
3301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
3361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
3421 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
3481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
3541 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
3601 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
3661 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
3721 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
3781 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
3841 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
3901 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
3961 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
4021 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
4081 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
4141 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
4201 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
4261 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
4321 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
4381 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
4441 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
4501 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
4561 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
4621 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
4681 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
4741 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
4801 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
4861 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
4921 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
4981 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
5041 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
5101 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
5161 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
5221 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
5281 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
5341 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
5401 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
5461 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
5521 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
5581 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
5641 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
5701 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
5761 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
5821 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
5881 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
5941 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
6001 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
6061 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
6121 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
6181 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
6241 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
6301 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
6361 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
6421 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
6481 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
6541 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
6601 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
6661 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
6721 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
6781 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
6841 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
6901 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
6961 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
7021 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
7081 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
7141 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
7201 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
7261 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
7321 gaaaagtgcc acctgacgtc
//
