| 出品公司: | Clontech |
|---|---|
| 载体名称: | pBridge |
| 质粒类型: | 酵母三杂交系统载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | ADH1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6526bp |
| 5' 测序引物及序列: | Match: 5'd[TCATCGGAAGAGAGTAGT]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | TRP1 |
| 备注: | Used as bait (& bridge) for yeast 3-hybrid screen. |
| 产品目录号: | 6184-1 |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | -- |
买家导航
pBridge载体质粒基本信息
pBridge质粒图谱载体图谱和pBridge载体序列质粒序列多克隆位点信息
pBridge质粒载体简介
pBridge质粒序列载体序列
LOCUS pBridge 6526 bp DNA SYN 30-Oct-2013
DEFINITION pBridge
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6526
/organism="pBridge"
/mol_type="other DNA"
promoter 10..406
/label="yADH1_promoter"
misc_feature 434..874
/label="Gal4_DBD"
misc_feature 838..856
/label="Gal4_N_term_primer"
misc_feature complement(976..1000)
/label="pHybLex_rev_primer"
terminator 921..1112
/label="yADH1_terminator"
CDS complement(1161..1835)
/label="TRP1"
/gene="TRP1"
/note="ORF frame 3"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
gene complement(1161..1835)
/label="TRP1"
/gene="TRP1"
misc_feature complement(1964..1986)
/label="M13_pUC_rev_primer"
promoter complement(2000..2029)
/label="lac_promoter"
rep_origin complement(3341..3960)
/label="pBR322_origin"
CDS complement(4115..4975)
/label="Ampicillin"
/gene="Ampicillin"
/note="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
gene complement(4115..4975)
/label="Ampicillin"
/gene="Ampicillin"
promoter complement(5017..5045)
/label="AmpR_promoter"
misc_feature complement(5204..5226)
/label="pGEX_3_primer"
rep_origin 5362..6526
/label="2micron2_origin"
ORIGIN
1 GCTTGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC
61 ATATCCGCAA TGACAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC
121 AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT
181 TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT
241 CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA
301 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT
361 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTGAAGCA
421 AGCCTCCTGA AAGATGAAGC TACTGTCTTC TATCGAACAA GCATGCGATA TTTGCCGACT
481 TAAAAAGCTC AAGTGCTCCA AAGAAAAACC GAAGTGCGCC AAGTGTCTGA AGAACAACTG
541 GGAGTGTCGC TACTCTCCCA AAACCAAAAG GTCTCCGCTG ACTAGGGCAC ATCTGACAGA
601 AGTGGAATCA AGGCTAGAAA GACTGGAACA GCTATTTCTA CTGATTTTTC CTCGAGAAGA
661 CCTTGACATG ATTTTGAAAA TGGATTCTTT ACAGGATATA AAAGCATTGT TAACAGGATT
721 ATTTGTACAA GATAATGTGA ATAAAGATGC CGTCACAGAT AGATTGGCTT CAGTGGAGAC
781 TGATATGCCT CTAACATTGA GACAGCATAG AATAAGTGCG ACATCATCAT CGGAAGAGAG
841 TAGTAACAAA GGTCAAAGAC AGTTGACTGT ATCGCCGGAA TTCCCGGGGA TCCGTCGACC
901 TGCAGCCAAG CTAATTCCGG GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG
961 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT
1021 TCTTGTTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG
1081 TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCGGCAAG TGCACAAACA ATACTTAAAT
1141 AAATACTACT CAGTAATAAC CTATTTCTTA GCATTTTTGA CGAAATTTGC TATTTTGTTA
1201 GAGTCTTTTA CACCATTTGT CTCCACACCT CCGCTTACAT CAACACCAAT AACGCCATTT
1261 AATCTAAGCG CATCACCAAC ATTTTCTGGC GTCAGTCCAC CAGCTAACAT AAAATGTAAG
1321 CTTTCGGGGC TCTCTTGCCT TCCAACCCAG TCAGAAATCG AGTTCCAATC CAAAAGTTCA
1381 CCTGTCCCAC CTGCTTCTGA ATCAAACAAG GGAATAAACG AATGAGGTTT CTGTGAAGCT
1441 GCACTGAGTA GTATGTTGCA GTCTTTTGGA AATACGAGTC TTTTAATAAC TGGCAAACCG
1501 AGGAACTCTT GGTATTCTTG CCACGACTCA TCTCCATGCA GTTGGACGAT ATCAATGCCG
1561 TAATCATTGA CCAGAGCCAA AACATCCTCC TTAGGTTGAT TACGAAACAC GCCAACCAAG
1621 TATTTCGGAG TGCCTGAACT ATTTTTATAT GCTTTTACAA GACTTGAAAT TTTCCTTGCA
1681 ATAACCGGGT CAATTGTTCT CTTTCTATTG GGCACACATA TAATACCCAG CAAGTCAGCA
1741 TCGGAATCTA GAGCACATTC TGCGGCCTCT GTGCTCTGCA AGCCGCAAAC TTTCACCAAT
1801 GGACCAGAAC TACCTGTGAA ATTAATAACA GACATACTCC AAGCTGCCTT TGTGTGCTTA
1861 ATCACGTATA CTCACGTGCT CAATAGTCAC CAATGCCCTC CCTCTTGGCC CTCTCCTTTT
1921 CTTTTTTCGA CCGAATTAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA
1981 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
2041 CTAATGAGTG AGGTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
2101 AAACCTGTCG TGCCAGGAAG ATCCGAGGCC TAGCTTCTAA TTCTTCCAAC ATACAATGGG
2161 AGTTTGGCCG AGTGGTTTAA GGCGTCAGAT TTAGGTGGAT TTAACCTCTA AAATCTCTGA
2221 TATCTTCGGA TGCAAGGGTT CGAATCCCTT AGCTCTCATT ATTTTTTGCT TTTTCTCTTG
2281 AGGTCACATG ATCGCAAAAT GGCAAATGGC ACGTGAAGCT GTCGATATTG GGGAACTGTG
2341 GTGGTTGGCA AATGACTAAT TAAGTTAGTC AAGGCGCCAT CCTCATGAAA ACTGTGTAAC
2401 ATAATAACCG AAGTGTCGAA AAGGTGGCAC CTTGTCCAAT TGAACACGCT CGATGAAAAA
2461 AATAAGATAT ATATAAGGTT AAGTAAAGCG TCTGTTAGAA AGGAAGTTTT TCCTTTTTCT
2521 TGCTCTCTTG TCTTTTCATC TACTATTTCC TTCGTGTAAT ACAGGGTCGT CAGATACATA
2581 GATACAATTC TATTACCCCC ATCCATACAA TGGGCCATAT GGCTTCTAGC TATCCTTATG
2641 ACGTGCCTGA CTATGCCAGC CTGGGAGGAC CTTCTAGTCC TAAGAAGAAG AGAAAGGTGG
2701 CGGCCGCATT AGCCCGAAGA TCTTCGGGCT GATCTCCCAT GTCTCTACTG GTGGTGGTGC
2761 TTCTTTGGAA TTATTGGAAG GTAAGGAATT GCCAGGTGTT GCTTTCTTAT CCGAAAAGAA
2821 ATAAATTGAA TTGAATTGAA ATCGATAGAT CAATTTTTTT CTTTTCTCTT TCCCCATCCT
2881 TTACGCTAAA ATAATAGTTT ATTTTATTTT TTGAATATTT TTTATTTATA TACGTATATA
2941 TAGACTATTA TTTATCTTTT AATGATTATT AAGATTTTTA TTAAAAAAAA ATTCGCTCCT
3001 CTTTTAATGC CTTTATGCAG TTTTTTTTTC CCATTCGATA TTTCTATGTT CGGGTTCAGC
3061 GTATTTTAAG TTTAATAACT CGAAAATTCT GCGTTCGTTA AAGCTAGGCC TCGGATCTTC
3121 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGTTT GCGTATTGGG CGCTCTTCCG
3181 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
3241 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
3301 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
3361 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
3421 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
3481 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
3541 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
3601 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
3661 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
3721 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
3781 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
3841 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
3901 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
3961 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
4021 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
4081 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
4141 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
4201 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
4261 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
4321 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
4381 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
4441 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
4501 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
4561 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
4621 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
4681 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
4741 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
4801 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
4861 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
4921 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
4981 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
5041 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
5101 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
5161 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
5221 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG
5281 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA
5341 TTGTACTGAG AGTGCACCAT AACGCATTTA AGCATAAACA CGCACTATGC CGTTCTTCTC
5401 ATGTATATAT ATATACAGGC AACACGCAGA TATAGGTGCG ACGTGAACAG TGAGCTGTAT
5461 GTGCGCAGCT CGCGTTGCAT TTTCGGAAGC GCTCGTTTTC GGAAACGCTT TGAAGTTCCT
5521 ATTCCGAAGT TCCTATTCTC TAGCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAAAC
5581 CAAAAGCGCT CTGAAGACGC ACTTTCAAAA AACCAAAAAC GCACCGGACT GTAACGAGCT
5641 ACTAAAATAT TGCGAATACC GCTTCCACAA ACATTGCTCA AAAGTATCTC TTTGCTATAT
5701 ATCTCTGTGC TATATCCCTA TATAACCTAC CCATCCACCT TTCGCTCCTT GAACTTGCAT
5761 CTAAACTCGA CCTCTACATT TTTTATGTTT ATCTCTAGTA TTACTCTTTA GACAAAAAAA
5821 TTGTAGTAAG AACTATTCAT AGAGTGAATC GAAAACAATA CGAAAATGTA AACATTTCCT
5881 ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA
5941 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAATA
6001 TAATCGGGGA TGCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT AATCAGTAAA
6061 CGCGGGAAGT GGAGTCAGGC TTTTTTTATG GAAGAGAAAA TAGACACCAA AGTAGCCTTC
6121 TTCTAACCTT AACGGACCTA CAGTGCAAAA AGTTATCAAG AGACTGCATT ATAGAGCGCA
6181 CAAAGGAGAA AAAAAGTAAT CTAAGATGCT TTGTTAGAAA AATAGCGCTC TCGGGATGCA
6241 TTTTTGTAGA ACAAAAAAGA AGTATAGATT CTTTGTTGGT AAAATAGCGC TCTCGCGTTG
6301 CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG
6361 TTGCATTTTT GTTTTACAAA AATGAAGCAC AGATTCTTCG TTGGTAAAAT AGCGCTTTCG
6421 CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC
6481 TCGCGTTGCA TTTTTGTTCT ACAAAATGAA GCACAGATGC TTCGTT
//
